Western Blot Reagents

In the Western blotting protocol, reagents such as blocking buffers are essential to getting clear and specific results as they prevent antibodies from binding to the blotting membrane nonspecifically. Rockland offers a variety of products made to be paired with western blot protocols in a variety of research applications.

Blocking Buffers

Made to prevent non-specific binding of antibodies to the blotting membrane that can lead to high background

Chemiluminescent Reagents

Use for horseradish peroxidase (HRP) based detection with a variety of protein targets while allowing for optimal signal-to-background ratio

Chromogenic Substrates

Use for sensitive colorimetric detection with a variety of protein targets

Fluorescent Reagents

Use for fluorescent detection with fluorochrome labeled antibodies to decrease background fluorescence and optimize signal

What is Western Blotting?

Western blotting is a widely used method for detection of a specific protein in a complex matrix, such as cell or tissue lysate (i.e. protein extracts). The Western blot assay uses gel electrophoresis (SDS-PAGE or native PAGE) to separate proteins according to molecular weight.

Western blot allows the transfer of proteins separated on a gel (SDS-PAGE) to a sticky membrane. Protein transfer is facilitated by electric current, negatively charged proteins and supporting reagents such as filter paper, transfer buffer, and a nitrocellulose or PVDF membrane. Detection of the target protein in a Western is carried out by antibodies. Various detection methods such as chemiluminescent or fluorescent detection are available.