Secondary Antibodies

Rockland offers a wide range of secondary antibody options for almost every possible experimental protocol. Our secondary antibodies are sourced from a variety of hosts in our onsite vivarium.

Secondary antibodies are used with various types of assays, including ELISA, Western blot (WB), immunohistochemistry (IHC), and flow cytometry (FC). The secondary antibody type is selected according to the class of the primary antibody (e.g., IgG or IgM), the source host, and the kind of label such as horseradish peroxidase (HRP). Most primary antibodies are of the IgG class and are produced in a common set of host species that includes rabbit, mouse, goat, or chicken.

Browse All Secondary Antibodies
Secondary Antibodies
Conjugated Antibodies
Browse our conjugated secondary antibodies for colorimetric, fluorescent, or chemiluminescent detection
Unconjugated Antibodies
Browse our unconjugated secondary antibody collection suitable for a wide range of applications
Pre-adsorbed Antibodies
Eliminate cross-reactivity with pre-absorbed antibodies ideal for multiple labeling or multiplex experiments
Fab Fragment Antibodies
Block endogenous IgGs and maximize permeability with Rockland's F(ab) and F(ab')2 fragment antibodies

 

Recommended Anti-Mouse Secondary Antibodies

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610-1319
Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-Adsorbed
Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-Adsorbed
40 References
Applications
ELISA, IHC, Other, WB
VIEW PRODUCT
610-145-002
Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated
Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated
46 References
Applications
Dot Blot, WB, IF, IHC
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610-1202
Mouse IgG (H&L) Antibody Fluorescein Conjugated
Mouse IgG (H&L) Antibody Fluorescein Conjugated
11 References
Applications
Dot Blot, WB, IF, IHC
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610-103-040
Mouse IgG1 Antibody Peroxidase Conjugated Pre-adsorbed
Mouse IgG1 Antibody Peroxidase Conjugated Pre-adsorbed
10 References
Applications
ELISA, IHC, WB
VIEW PRODUCT

Recommended Anti-Rabbit Secondary Antibodies

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611-1302
Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated
Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated
86 References
Applications
ELISA, WB, IHC
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611-103-122
Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed
Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed
27 References
Applications
Dot Blot, ELISA, WB, IHC
VIEW PRODUCT
611-144-002
Rabbit IgG (H&L) Antibody DyLight™ 680 Conjugated
Rabbit IgG (H&L) Antibody DyLight™ 680 Conjugated
Applications
Dot Blot, WB, IP
VIEW PRODUCT
611-145-122
Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated Pre-Adsorbed
Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated Pre-Adsorbed
Applications
Dot Blot, Other, WB
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Recommended Anti-Goat Secondary Antibodies

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605-4302
Goat IgG (H&L) Secondary Antibody Peroxidase Conjugated
Goat IgG (H&L) Secondary Antibody Peroxidase Conjugated
21 References
Applications
ELISA, IHC, WB
VIEW PRODUCT
605-4313
Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
Goat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
6 References
Applications
ELISA, WB
VIEW PRODUCT
605-744-002
Goat IgG (H&L) Antibody DyLight™ 680 Conjugated
Goat IgG (H&L) Antibody DyLight™ 680 Conjugated
2 References
Applications
Dot Blot, WB
VIEW PRODUCT
605-4602
Goat IgG (H&L) Secondary Antibody Biotin Conjugated
Goat IgG (H&L) Secondary Antibody Biotin Conjugated
2 References
Applications
ELISA, IHC, Microarray
VIEW PRODUCT

 

Resources

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Tips for Selecting the Best Secondary Antibody

Technical Tips
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DyLight Fluorescent Dye Conjugates

Literature
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Tips for Selecting Conjugation Methods

Technical Tips
View Technical Tip

How to Choose the Best Secondary Antibody

Choosing a secondary antibody is straightforward. Simply select a secondary antibody that recognizes the host species used to produce the primary antibody of interest (reactivity). For example, choose a goat anti-mouse secondary antibody for the detection of a mouse monoclonal primary antibody.

Then, match the class/subclass of the primary antibody (specificity). For example, polyclonal primary antibodies are usually gamma chain immunoglobulins (IgG isotype). Therefore, the secondary antibody should be an anti-IgG antibody that detects both heavy and light chains (anti-IgG H&L). 

Identifying the optimal secondary antibody also requires knowledge of the detection assay. For example, Western blotting and ELISA can be performed using colorimetric, chemiluminescent, and fluorescence reporter systems, while immunofluorescence and flow cytometry are generally limited to fluorescent reporter labels. In all of these instances, a conjugated secondary antibody is required (conjugation).

 

Select

  1. Reactivity (depending on the primary antibody)
  2. Specificity (depending on the primary antibody)
  3. Conjugation (depending on the intended use such as Western blotting or flow cytometry)
  4. Format (consider Fab and F(ab')2 fragment antibodies for certain applications)
  5. Consider pre-adsorbed antibodies for minimal cross-reactivity

 

Want more tips for choosing a secondary antibody? Read the full list of tips in our resource library and download our brochure "Tips for Selecting the Best Secondary Antibody."


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