Datasheet is currently unavailable. Try again or CONTACT US

Blocking Buffer (2X) For Fluorescent Western Blotting

50 mL
Have a Question? Ask a Specialist
$48.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.



Western Blot Blocking Buffer is ideal for infrared Western Blotting. Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background.  IRDye™800, IRDye™700DX, Alexa Fluor® 680 and Cy5.5™ antibody and reagent conjugates are specifically designed for protein detection methods that use longer-wavelength, near-infrared (IR) fluorophores to visualize proteins in western blotting and other applications.  IRDye™800 and IRDye™700DX fluoresce outside the range of most autofluorescence and therefore specific signal is sharply evident from any background giving the best possible signal-to-noise ratio.  Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film.  IRDye™800 conjugates are also suitable for immunofluorescence microscopy using commercially available excitation/emission filters in the 780nm/820nm range.  Dual simultaneous labeling in western blots or microscopy is achieved when IRDye™800 conjugates are used in conjunction with IRDye™700 or Cy5.5™ conjugates. IRDye™800 and IRDye™700DX conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging. Once reacted with the membrane and dried, IRDye™800 and IRDye™700DX conjugated antibody-protein complexes are very stable, and membranes can be stored protected from light, re-washed and/or rescanned.


Multiplex Blocking Buffer, Fluorescent Blocking Buffer, Blocking Solution, Blocking Buffer Western Blot, IRDye Western Blot Blocking Buffer, Alexa Dye Blocking Buffer, DyLight Blocking Buffer

Application Note

This product is a 2X concentrated stock solution.  Prepare a 1X working solution by diluting 1 part 2X concentrate with 1 parts TBS or equivalent. This buffer was meticulously prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.


Blocking buffer is specifically formulated to achieve superior reproducible western blotting images using this system.

Storage Condition

Store blocking buffer at 4° C prior to opening. DO NOT FREEZE.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Blocking Buffer (2X) for Fluorescent Western Blotting - MB-070-0050
Liquid (sterile filtered)
Wet Ice
Expiration date is six (6) months from date of opening.
See application note.
Thimerosal is added as an antimicrobial agent.

Invalid lot number

If you need help finding your CofA, contact us

  • Epitopes of Naturally Acquired and Vaccine-Induced Anti-Ebola Virus Glycoprotein Antibodies in Single Amino Acid Resolution.;2020;Heidepriem J. et al.;PubMed
  • Versicolorin A, a precursor in aflatoxins biosynthesis, is a food contaminant toxic for human intestinal cells.;2020;Gauthier T. et al.;PubMed
  • Expression and Function of Mas-Related G Protein-Coupled Receptor D and Its Ligand Alamandine in Retina.;2020;Zhu P. et al.;PubMed
  • A proteolytic C-terminal fragment of Nogo-A (reticulon-4A) is released in exosomes and potently inhibits axon regeneration.;2020;Sekine Y, Lindborg JA, Strittmatter SM.;PubMed
  • The stress-responsive gene GDPGP1/mcp-1 regulates neuronal glycogen metabolism and survival.;2020;Schulz A, Sekine Y, Oyeyemi MJ, et al.;PubMed
  • Pre-clinical characterisation of E2814, a high-affinity antibody targeting the microtubule-binding repeat domain of tau for passive immunotherapy in Alzheimer's disease.;2020;Roberts M, Sevastou I, Imaizumi Y, et al.;PubMed
  • Immunization with full-length Plasmodium falciparum merozoite surface protein 1 is safe and elicits functional cytophilic antibodies in a randomized first-in-human trial.;2020;Blank A, Fürle K, Jäschke A, et al.;PubMed
  • Human HMGN1 and HMGN2 are not required for transcription-coupled DNA repair.;2020;Apelt K, Zoutendijk I, Gout DY, Wondergem AP, van den Heuvel D, Luijsterburg MS.;PubMed
  • On-Chip Neo-Glycopeptide Synthesis for Multivalent Glycan Presentation.;2020;Mende M, Tsouka A, Heidepriem J, et al.;PubMed
  • The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II.;2020;van der Weegen Y, Golan-Berman H, Mevissen TET, et al.;PubMed
  • TrkB agonists prevent postischemic emergence of refractory neonatal seizures in mice.;2020;Kipnis PA, Sullivan BJ, Carter BM, Kadam SD.;PubMed
  • A cross-reactive mouse monoclonal antibody against rhinovirus mediates phagocytosis in vitro.;2020;Behzadi MA, Choi A, Duehr J, et al.;PubMed
  • Hypoxia regulates the degradation of non-nuclear organelles during lens differentiation through activation of HIF1a;2020;Brennan L, Disatham J, Kantorow M.;PubMed
  • Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity.;2020;Fu Y, Cao R, Schäfer M, et al.;PubMed
  • Identification of truncated C-terminal fragments of the Alzheimer's disease amyloid protein precursor derived from sequential proteolytic pathways.;2020;Mosser S, Gerber H, Fraering PC.;PubMed
  • Small-molecule inhibition of Lats kinases promotes Yap-dependent proliferation in postmitotic mammalian tissues.;2020;Nathaniel Kastan, Ksenia Gnedeva, Theresa Alisch, Aleksandra A. Petelski, David J. Huggins, Jeanne C;PubMed
  • Cardiac Cell-Derived Matrices Impart Age-Specific Functional Properties to Human Cardiomyocytes.;2020;M. A. Kauss, S. J. Rockwood, A. C. Silva, D. A. Joy, N. Mendoza-Camacho, M. N. Whittaker, Erica Stev;PubMed
  • Trem2 Deletion Reduces Late-Stage Amyloid Plaque Accumulation, Elevates the Aβ42:Aβ40 Ratio, and Exacerbates Axonal Dystrophy and Dendritic Spine Loss in the PS2APP Alzheimer's Mouse Model.;2020;Meilandt WJ, Ngu H, Gogineni A, et al.;PubMed
  • SNX27 regulates DRA activity and mediates its direct recycling by PDZ-interaction in early endosomes at the apical pole of Caco2 cells.;2020;Bannert K, Berlin P, Reiner J, et al.;PubMed
  • Organic dust induced mitochondrial dysfunction could be targeted via cGAS-STING or mitochondrial NOX-2 inhibition.;2020;Massey N. et al.;PubMed
  • In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation.;2019;Spence et al.;PubMed
  • Amplified Host Defense by Toll-Like Receptor-Mediated Downregulation of the Glucocorticoid-Induced Leucine Zipper (GILZ) in Macrophages.;2019;Hoppstädter et al.;PubMed
  • Alzheimer's Disease Risk Factor Pyk2 Mediates Amyloid-β-Induced Synaptic Dysfunction and Loss.;2019;Salazar SV, Cox TO, Lee S, et al.;PubMed
  • Lipidomic Analysis of α-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment.;2019;Fanning S, Haque A, Imberdis T, et al.;PubMed
  • Maelstrom Represses Canonical Polymerase II Transcription within Bi-directional piRNA Clusters in Drosophila melanogaster.;2019;Chang TH, Mattei E, Gainetdinov I, Colpan C, Weng Z, Zamore PD.;PubMed
  • Pyk2 Signaling through Graf1 and RhoA GTPase Is Required for Amyloid-β Oligomer-Triggered Synapse Loss.;2019;Lee S, Salazar SV, Cox TO, Strittmatter SM.;PubMed
  • Rescue of Transgenic Alzheimer's Pathophysiology by Polymeric Cellular Prion Protein Antagonists.;2019;Gunther EC, Smith LM, Kostylev MA, et al.;PubMed
  • Plexina2 and CRMP2 Signaling Complex Is Activated by Nogo-A-Liganded Ngr1 to Restrict Corticospinal Axon Sprouting after Trauma.;2019;Sekine Y, Algarate PT, Cafferty WBJ, Strittmatter SM.;PubMed
  • High-throughput epitope profiling of antibodies in the plasma of Alzheimer's disease patients using random peptide microarrays.;2019;Sim KY, Park SH, Choi KY, et al.;PubMed
  • Anti-PrPC antibody rescues cognition and synapses in transgenic alzheimer mice.;2019;Cox TO, Gunther EC, Brody AH, et al.;PubMed
  • Immunization of cats to induce neutralizing antibodies against Fel d 1, the major feline allergen in human subjects.;2019;Thoms F, Jennings GT, Maudrich M, et al.;PubMed
  • The RNA-Binding ATPase, Armitage, Couples piRNA Amplification in Nuage to Phased piRNA Production on Mitochondria.;2019;Ge DT, Wang W, Tipping C, Gainetdinov I, Weng Z, Zamore PD.;PubMed
  • A Monoclonal Antibody to M-Phase Phosphoprotein 1/Kinesin-Like Protein KIF20B.;2019;Fritzler MJ, Brown RD, Zhang M.;PubMed
  • Toll-Like Receptor 2 Release by Macrophages: An Anti-inflammatory Program Induced by Glucocorticoids and Lipopolysaccharide.;2019;Hoppstädter J, Dembek A, Linnenberger R, et al.;PubMed
  • Mitochondrial calcium exchange links metabolism with the epigenome to control cellular differentiation.;2019;Lombardi AA, Gibb AA, Arif E, et al.;PubMed
  • Biphasic Response of Protein Kinase A to Cyclic Adenosine Monophosphate Triggers Distinct Epithelial Phenotypes.;2019;João Pedro Fonseca, Elham Aslankoohi, Hana El-Samad;PubMed
  • MICU1 regulates mitochondrial cristae structure and function independent of the mitochondrial calcium uniporter channel.;2019;Dhanendra Tomar, Manfred Thomas, Joanne F. Garbincius, Devin W. Kolmetzky, Oniel Salik, Pooja Jadiy;PubMed
  • mitoTev-TALE: a monomeric DNA editing enzyme to reduce mutant mitochondrial DNA levels.;2018;Pereira et al.;PubMed
  • Identification of the antigenic epitopes of maternal autoantibodies in autism spectrum disorders.;2018;Edmiston E, Jones KL, Vu T, Ashwood P, Van de Water J.;PubMed
  • The nociceptin receptor inhibits axonal regeneration and recovery from spinal cord injury.;2018;Sekine Y, Siegel CS, Sekine-Konno T, Cafferty WBJ, Strittmatter SM.;PubMed
  • Protein Corona in Response to Flow: Effect on Protein Concentration and Structure.;2018;Jayaram DT, Pustulka SM, Mannino RG, Lam WA, Payne CK.;PubMed
  • Mycobacterium tuberculosis-specific CD4+ and CD8+ T cells differ in their capacity to recognize infected macrophages.;2018;Yang JD, Mott D, Sutiwisesak R, et al.;PubMed
  • BNIP3L/NIX is required for elimination of mitochondria, endoplasmic reticulum and Golgi apparatus during eye lens organelle-free zone formation.;2018;Brennan LA, McGreal-Estrada R, Logan CM, Cvekl A, Menko AS, Kantorow M.;PubMed
  • Combinatorial Synthesis of Macromolecular Arrays by Microchannel Cantilever Spotting (µCS).;2018;Atwater J, Mattes DS, Streit B, et al.;PubMed
  • Thrombospondin receptor α2δ-1 promotes synaptogenesis and spinogenesis via postsynaptic Rac1.;2018;Risher WC, Kim N, Koh S, et al.;PubMed
  • A Single Mechanism of Biogenesis, Initiated and Directed by PIWI Proteins, Explains piRNA Production in Most Animals.;2018;Gainetdinov I, Colpan C, Arif A, Cecchini K, Zamore PD.;PubMed
  • Generation and characterization of monoclonal antibodies that recognize human and murine supervillin protein isoforms.;2018;Smith TC, Saul RG, Barton ER, Luna EJ.;PubMed
  • Liquid and Hydrogel Phases of PrPC Linked to Conformation Shifts and Triggered by Alzheimer's Amyloid-β Oligomers.;2018;Kostylev MA, Tuttle MD, Lee S, et al.;PubMed
  • KDEL Receptors Are Differentially Regulated to Maintain the ER Proteome under Calcium Deficiency.;2018;Trychta KA, Bäck S, Henderson MJ, Harvey BK.;PubMed
  • Development of Neutralizing and Non-neutralizing Antibodies Targeting Known and Novel Epitopes of TcdB of Clostridioides difficile.;2018;Fühner V, Heine PA, Helmsing S, et al.;PubMed
  • Interaction of the Human Respiratory Syncytial Virus matrix protein with cellular adaptor protein complex 3 plays a critical role in trafficking.;2017;Ward et al.;PubMed
  • Early Activation of Experience-Independent Dendritic Spine Turnover in a Mouse Model of Alzheimer's Disease.;2017;Heiss JK, Barrett J, Yu Z, Haas LT, Kostylev MA, Strittmatter SM.;PubMed
  • Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase.;2017;Gupta P, Lavagnolli T, Mira-Bontenbal H, Merkenschlager M.;PubMed
  • Opposing effects of progranulin deficiency on amyloid and tau pathologies via microglial TYROBP network.;2017;Takahashi H, Klein ZA, Bhagat SM, et al.;PubMed
  • Induction of Immune Tolerance to Foreign Protein via Adeno-Associated Viral Vector Gene Transfer in Mid-Gestation Fetal Sheep.;2017;Davey MG, Riley JS, Andrews A, et al.;PubMed
  • A biomimetic gelatin-based platform elicits a pro-differentiation effect on podocytes through mechanotransduction.;2017;Hu M, Azeloglu EU, Ron A, et al.;PubMed
  • Lifespan differences between queens and workers are not explained by rates of molecular damage.;2017;Lucas ER, Augustyniak M, Kędziorski A, Keller L.;PubMed
  • The C Terminus of the Herpes Simplex Virus UL25 Protein Is Required for Release of Viral Genomes from Capsids Bound to Nuclear Pores.;2017;Huffman JB, Daniel GR, Falck-Pedersen E, et al.;PubMed
  • Nanoparticle-induced oxidation of corona proteins initiates an oxidative stress response in cells.;2017;Jayaram DT, Runa S, Kemp ML, Payne CK.;PubMed
  • Loss of TMEM106B Ameliorates Lysosomal and Frontotemporal Dementia-Related Phenotypes in Progranulin-Deficient Mice.;2017;Klein ZA, Takahashi H, Ma M, et al.;PubMed
  • Systematic and Quantitative Assessment of Hydrogen Peroxide Reactivity With Cysteines Across Human Proteomes.;2017;Fu L, Liu K, Sun M, et al.;PubMed
  • Conditional Deletion of Prnp Rescues Behavioral and Synaptic Deficits after Disease Onset in Transgenic Alzheimer's Disease.;2017;Salazar SV, Gallardo C, Kaufman AC, et al.;PubMed
  • Identification of two conserved B-cell epitopes in the gp90 of reticuloendothelial virus using peptide microarray.;2017;Khairy WOA, Qian K, Shao H, Ye J, Qin A.;PubMed
  • Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses.;2017;Freire MCLC, Pol-Fachin L, Coêlho DF, et al.;PubMed
  • Metabotropic glutamate receptor 5 couples cellular prion protein to intracellular signalling in Alzheimer's disease.;2016;Haas LT, Salazar SV, Kostylev MA, Um JW, Kaufman AC, Strittmatter SM.;PubMed
  • Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis.;2016;Metz I, Beißbarth T, Ellenberger D, et al.;PubMed
  • Identification of a Highly Conserved Epitope on Avian Influenza Virus Non-Structural Protein 1 Using a Peptide Microarray.;2016;Sun J, Wang X, Wen X, et al.;PubMed
  • Assembly Dynamics and Stoichiometry of the Apoptosis Signal-regulating Kinase (ASK) Signalosome in Response to Electrophile Stress.;2016;Federspiel JD, Codreanu SG, Palubinsky AM, et al.;PubMed
  • High-Density Peptide Arrays for Malaria Vaccine Development.;2016;Loeffler FF, Pfeil J, Heiss K.;PubMed
  • Properties of Two Enterovirus Antibodies that are Utilized in Diabetes Research.;2016;Maccari G, Genoni A, Sansonno S, Toniolo A.;PubMed
  • High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material.;2016;Loeffler FF, Foertsch TC, Popov R, et al.;PubMed
  • Oligomers of Amyloid β Prevent Physiological Activation of the Cellular Prion Protein-Metabotropic Glutamate Receptor 5 Complex by Glutamate in Alzheimer Disease.;2016;Haas LT, Strittmatter SM.;PubMed
  • A single amino acid substitution alter antigenicity of Glycosylated protein 4 of HP-PRRSV.;2016;Wang X, Wang Z, Xu H, Biao X, Yang Z.;PubMed
  • Transient Hepatic Overexpression of Insulin-Like Growth Factor 2 Induces Free Cholesterol and Lipid Droplet Formation.;2016;Kessler SM, Laggai S, Van Wonterghem E, et al.;PubMed
  • Increased Intimal Hyperplasia After Vascular Injury in Male Androgen Receptor-Deficient Mice.;2016;Wilhelmson AS, Fagman JB, Johansson I, et al.;PubMed
  • Identification of novel antigens contributing to autoimmunity in cardiovascular diseases.;2016;Müller AM, Bockstahler M, Hristov G, et al.;PubMed
  • Validation of Cross-Species Reactivity of the VEGF-A/PDGFRβ Bifunctional Antibody PF-06653157.;2016;Ashoor R, Lee D, Cheng A, Jessen B, Huang W.;PubMed
  • Dynamic Phosphorylation of Apoptosis Signal Regulating Kinase 1 (ASK1) in Response to Oxidative and Electrophilic Stress.;2016;Morales Betanzos C, Federspiel JD, Palubinsky AM, McLaughlin B, Liebler DC.;PubMed
  • TiO2 Nanoparticles Alter the Expression of Peroxiredoxin Antioxidant Genes.;2016;Sabiha Runa, Dipesh Khanal, Melissa L. Kemp, and Christine K. Payne.;PubMed
  • Pattern recognition receptors in the gut: analysis of their expression along the intestinal tract and the crypt/villus axis.;2015;Gourbeyre P, Berri M, Lippi Y, et al.;PubMed
  • Spot peptide arrays and SPR measurements: throughput and quantification in antibody selectivity studies.;2015;Vernet T, Choulier L, Nominé Y, et al.;PubMed
  • Cross-Talk between Carbon Metabolism and the DNA Damage Response in S. cerevisiae.;2015;Simpson-Lavy KJ, Bronstein A, Kupiec M, Johnston M.;PubMed
  • Huntingtin is required for normal excitatory synapse development in cortical and striatal circuits.;2014;McKinstry SU, Karadeniz YB, Worthington AK, et al.;PubMed
  • Lysosome size, motility and stress response regulated by fronto-temporal dementia modifier TMEM106B.;2014;Stagi M, Klein ZA, Gould TJ, Bewersdorf J, Strittmatter SM.;PubMed
  • Isolation and characterization of the herpes simplex virus 1 terminase complex.;2014;Heming JD, Huffman JB, Jones LM, Homa FL.;PubMed
  • Analysis of autoantibodies to 3-hydroxy-3-methylglutaryl-coenzyme A reductase using different technologies.;2014;Musset L, Miyara M, Benveniste O, et al.;PubMed
  • Antigenic characteristics of glycosylated protein 3 of highly pathogenic porcine reproductive and respiratory syndrome virus.;2014;Wang X, Dang R, Liu W, Yang Z, Du E, Zhang S.;PubMed
  • Redox processes inform multivariate transdifferentiation trajectories associated with TGFβ-induced epithelial-mesenchymal transition.;2014;Prasanphanich AF, Arencibia CA, Kemp ML.;PubMed
  • Therapeutic molecules and endogenous ligands regulate the interaction between brain cellular prion protein (PrPC) and metabotropic glutamate receptor 5 (mGluR5).;2014;Haas LT, Kostylev MA, Strittmatter SM.;PubMed
  • Linear epitope recognition antibodies strongly respond to the C-terminal domain of HP-PRRSV GP5.;2014;Wang X, Qui L, Dang Y, Xiao S, Zhang S, Yang Z.;PubMed
  • Autophagy-dependent PELI3 degradation inhibits proinflammatory IL1B expression.;2014;Giegerich AK, Kuchler L, Sha LK, et al.;PubMed
  • Astrocytes refine cortical connectivity at dendritic spines.;2014;Risher WC, Patel S, Kim IH, et al.;PubMed
  • SUMOylation regulates the SNF1 protein kinase.;2013;Simpson-Lavy KJ, Johnston M.;PubMed
  • WhiB7, an Fe-S-dependent transcription factor that activates species-specific repertoires of drug resistance determinants in actinobacteria.;2013;Ramón-García S, Ng C, Jensen PR, et al.;PubMed
  • Mutational analysis of sclerostin shows importance of the flexible loop and the cystine-knot for Wnt-signaling inhibition.;2013;Boschert V, van Dinther M, Weidauer S, et al.;PubMed
  • Alzheimer amyloid-β oligomer bound to postsynaptic prion protein activates Fyn to impair neurons.;2012;Um JW, Nygaard HB, Heiss JK, et al.;PubMed
  • Sensing immune responses with customized peptide microarrays.;2012;Schirwitz C, Loeffler FF, Felgenhauer T, Stadler V, Breitling F, Bischoff FR.;PubMed
  • Residues of the UL25 protein of herpes simplex virus that are required for its stable interaction with capsids.;2011;Cockrell SK, Huffman JB, Toropova K, Conway JF, Homa FL.;PubMed
  • Endo-lysosomal vesicles positive for Rab7 and LAMP1 are terminal vesicles for the transport of dextran.;2011;Humphries WH 4th, Szymanski CJ, Payne CK.;PubMed
  • Coordinated regulation of extracellular matrix synthesis by the microRNA-29 family in the trabecular meshwork.;2011;Villarreal G Jr, Oh DJ, Kang MH, Rhee DJ.;PubMed