UniGlow™ - One Component Chemiluminescent Substrate
Western Blot using UniGlow - One Component Chemiluminescent Substrate
UniGlow™ - One Component Chemiluminescent Substrate
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Datasheet

UniGlow™ - One Component Chemiluminescent Substrate

UniGlow-0020 UniGlow-0100
20 mL 100 mL
ELISA, WB
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$66.00 /Per Item
$170.00 /Per Item
20 mL $66.00
100 mL $170.00
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Description

Background

UniGlow™ is designed as a ready-to-use chemiluminescent substrate in a One Component System. This is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). UniGlow easily allows for the detection of picograms (6-12 pg) amounts of antigen using photographic film or other imaging methods. UniGlow is a great quality product with the high sensitivity and excellent signal to noise ratios and low backgrounds. UniGlow substrate is highly stable.

Application Note

UniGlow™ is ready-to-use and no mixing is required. UniGlow™- One Component Chemiluminescent Substrate is for use in microwell or membrane applications. Protect from light - UniGlow™ Substrate is a highly sensitive detection reagent. Equilibrate to room temperature before use and aliquot into a clean container. Use ~100µL/cm2 of membrane. Optimal detection visualized after contact of substrate with HRP enzyme on membrane for ~1-10min. Blot off excess substrate before imaging. Always carefully optimize all components of individual assays (antigens, antibodies, conjugates…) to minimize background reactivity associated with non-specific binding.

UniGlow™ - One Component Chemiluminescent Substrate - UniGlow-0020
Western Blot detection, chemiluminescent substrate One Component System, luminol-based chemiluminescent substrate for horseradish peroxidase (HRP) detection
ELISA, WB
Liquid - light red colored
1X
Ambient
Store container at 2-8° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.
Expiration date is two (2) years from date of opening.
Shrestha RL et al. CENP-A overexpression promotes aneuploidy with karyotypic heterogeneity. J Cell Biol. (2021)
Applications
WB, IB, PCA
Ghosh S et al. A new microchannel capillary flow assay (MCFA) platform with lyophilized chemiluminescence reagents for a smartphone-based POCT detecting malaria. Nature. (2020)
Applications
E, EIA
Trabbic K., Whalen K., Abarca-Heideman K., Xia L., Temme J., Edmondson E., Gilersleeve J., Barchi J. A Tumor-Selective Monoclonal Antibody from Immunization with a Tumor-Associated Mucin Glycopeptide. Nature Scientific Reports. (2019)
Applications
WB, IB, PCA
Ohkuni K et al. N-terminal sumoylation of centromeric histone H3 variant Cse4 regulates its proteolysis to prevent mislocalization to non-centromeric chromatin. G3 (Bethesda). (2018)
Applications
WB, IB, PCA
Shrestha RL et al. Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells. Oncotarget. (2017)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.