Blotto Immunoanalytical Grade (Non-Fat Dry Milk)
Blotto Immunoanalytical Grade Bottles
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Datasheet

Blotto Immunoanalytical Grade (Non-Fat Dry Milk)

B501-0500
500 g
WB, Biochemical Assay, ELISA, IP
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$81.00 /Per Item
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Description

Background

Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below).  These products are intended to block non-specific binding of proteins in various immunological assays.  Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to the addition of primary antibody.

Application Note

Use Rockland's BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk.   Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available.  BLOTTO A is a convenient general purpose blocking agent.  BLOTTO B is recommended for blocking when phospho-specific antibodies are used.  BLOTTO A and BLOTTO B provide Tween-20 in a separate vial in liquid form (p/n TW0025).  Users should consider the addition of protease inhibitors and phosphatase inhibitors when required.  Typically 50 mM NaF can be added to BLOTTO solutions to inhibit phosphatase activity.

Purity/Specificity

This product contains highly purified immunoanalytical grade non-fat dry milk.

Storage Condition

Store container at room temperature prior to opening. After reconstitution, use blocking buffers immediately. Dilute solutions may be stored at 4° C for up to four (4) days. Solutions containing BLOTTO may be frozen.

BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500
Blotto Immunoanalytical Grade Non-Fat Dry Milk, Blocking reagent for western, antibody dilution buffer
WB
Biochemical Assay, ELISA, IP
Lyophilized
Ambient
Expiration date is one (1) year from date of opening.
None
None
None
Restore with deionized water (or equivalent)
Choi S et al. Polyhexamethylene guanidine phosphate increases stress granule formation in human 3D lung organoids under respiratory syncytial virus infection. (2022)
Applications
WB, IB, PCA
Aronson, M.R., Dahl, E.S., Halle, J.A. et al. Re-engineering Antimicrobial Peptides into Oncolytics Targeting Drug-Resistant Ovarian Cancers. (2020)
Applications
Undefined
Maki RA, Holzer M, Motamedchaboki K, et al. Human myeloperoxidase (hMPO) is expressed in neurons in the substantia nigra in Parkinson's disease and in the hMPO-α-synuclein-A53T mouse model, correlating with increased nitration and aggregation of α-synuclein and exacerbation of motor impairment. (2019)
Applications
IP, Co-IP; WB, IB, PCA; In Vitro Binding Assay
Ferko MA et al. Effects of metal ions on caspase-1 activation and interleukin-1β release in murine bone marrow-derived macrophages. (2018)
Applications
WB, IB, PCA
Pardee K et al. Portable, on-demand biomolecular manufacturing. (2016)
Applications
WB, IB, PCA
Laura RP et al. T47D Cells Expressing Myeloperoxidase Are Able to Process, Traffic and Store the Mature Protein in Lysosomes: Studies in T47D Cells Reveal a Role for Cys319 in MPO Biosynthesis that Precedes Its Known Role in Inter-Molecular Disulfide Bond Formation. (2016)
Applications
E, EIA; WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.