Rockland’s chromatin immunoprecipitation (ChIP) assay kits contain optimized reagents and protocols, making them quick and accurate. Our ChIP kits can be used to study changes in epigenetic signatures, chromatin remodelling, and transcription regulator recruitment to specific genomic sites.
How do ChIP assays work?
ChIP assay protocols involve the immunoprecipitation of protein/DNA complexes that are stabilized through cross-linking. The first step is cell fixation (cross-linking) where intact cells are fixed using formaldehyde, which is a popular fixing reagent that preserves protein/DNA interactions. Chromatin is then sheared into small uniform fragments, and the DNA/protein complexes are immunoprecipitated using an antibody directed against the DNA-binding protein of interest. Afterward, the DNA is washed, cross-linking is reversed, and the proteins are removed by Proteinase K treatment. This is followed by high-throughput sequencing using a Next-Gen sequencer.
Rockland also offers ChIP assay kits that target histones, transcription factors, as well as a ChIP kit targeted for small sample amounts to enable successful ChIP on as few as 10,000 cells.
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