Pre-adsorption (also cross-adsorption) of the secondary antibody is used to eliminate reactivity to IgG from undesired host species or antibody fragments. The degree of cross-reactivity is determined by ELISA and is typically less than 1% of the desired signal. The secondary antibody is cross-adsorbed against serum antibody-protein from another species or is adsorbed against a mixture of serum antibody-protein from several species (i.e., pre-adsorbed). These cross-adsorbed antibodies show low levels of cross-reactivity in multiple labeling or multiplex experiments. Cross-reactivity of pre-adsorbed secondary antibodies is determined by ELISA or Western blot detection.
Below are our most popular pre-adsorbed secondary antibodies by class and subclass.
Many antibodies are offered as pre-adsorbed against serum proteins from another species or are adsorbed against a mixture of serum proteins from several species (ie. designated "X to Ch, GP, Ham, Hs, Ms, Rb & Rt"). These highly cross-adsorbed secondary antibodies show extremely low levels of cross-reactivity in multiple labeling experiments. The degree of cross-reactivity is determined by ELISA and is typically less than 1% of the desired signal allowing for the use of several primary antibodies for different host species in one assay..
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