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Bovine Serum Albumin - Fraction V

53 References
BSA-10 BSA-50 BSA-1000 BSA-5kg
10 g 50 g 1 kg 5 x 1 kg
Lyophilized Lyophilized Lyophilized Lyophilized
WB, ELISA, IHC, IF, FC, Functional Assay, LFA
Bovine
$94.00 /Per Item
$132.00 /Per Item
$1,225.00 /Per Item
$5,215.00 /Per Item
10 g $94.00
50 g $132.00
1 kg $1,225.00
5 x 1 kg $5,215.00
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Background

Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screening assays, western blotting, FACS Buffer and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.

Product Details

Bovine Serum Albumin - Fraction V (Immunoglobulin and Protease Free) - BSA-10
Bovine serum albumin fraction V, Bovine Albumin, BSA, BSA Blocker, BSA Blocking
Bovine

Target Details

Purity (%): 100% by Agarose Zone electrophoresis Moisture (%) 5% Loss on Drying pH: 7.0 Ash: <2.0% Protein (%): 98% by nitrogen analysis Protease: <0.005 Units/mg Heavy Metals: (Pb) < 10 ppm by ICP Endotoxin: < 3 EU/mg by LAL IgG: Not Detected NEFA: N/A

Application Details

ELISA, FC, Functional Assay, IF, IHC, WB, LFA  - View References
Bovine Serum Albumin - Fraction V is suitable for use in lateral flow, in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes including dilute solutions of antibody, use as a blocking agent to reduce non-specific binding for FACS, IF, IHC, and WB.

Formulation

None
None
None
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store container at 4° C prior to opening.
Expiration date is one (1) year from date of receipt.
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Blocking
(). Single-cell RNA sequencing of murine ankle joints over time reveals distinct transcriptional changes following Borrelia burgdorferi infection. iScience.
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Buffer
(). The mRNA-binding protein DDX3 mediates TGF-β1 upregulation of translation and promotes pulmonary fibrosis. JCI Insight.
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Buffer
(). Soluble α-klotho and heparin modulate the pathologic cardiac actions of fibroblast growth factor 23 in chronic kidney disease. Kidney Int.
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IF, Confocal Microscopy; IHC, ICC, Histology
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Buffer; Blocking
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Blocking
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SDS, 2D-PAGE
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IF, Confocal Microscopy
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Hemagglutination Inhibition (HI) Assay
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IF, Confocal Microscopy
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Blocking
(). Commercial immunoglobulin products contain cross-reactive but not neutralizing antibodies against SARS-CoV-2. J Allergy Clin Immunol.
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E, EIA
(). Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen. Cell Reports
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FC, FACS, FLOW; Buffer
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Control; Blocking
(). DYNC1LI2 regulates localization of the chaperone-mediated autophagy receptor LAMP2A and improves cellular homeostasis in cystinosis. Autophagy.
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IHC, ICC, Histology
(). The Aurora B gradient sustains kinetochore stability in anaphase. Cell Rep.
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(). Application of Force to a Syndecan-4 Containing Complex With Thy-1–αVβ3 Integrin Accelerates Neurite Retraction. Front Mol Biosci.
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EIA, ELISA
(). Diminution of Phagocytosed Micro/Nanoparticles by Tethering with Immunoregulatory CD200 Protein. Sci Rep.
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WB, IB, PCA
(). Blockade of Macrophage Adhesion to CD200-treated Polystyrene Culture Surface. J Biomed Mater Res A.
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WB, IB, PCA
(). A three-dimensional dementia model reveals spontaneous cell cycle re-entry and a senescence-associated secretory phenotype. Neurobiol Aging.
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IF, Confocal Microscopy
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(). Developing a mass spectrometry-based assay for the ovarian cancer biomarker CA125 (MUC16) using suspension trapping (STrap). Anal Bioanal Chem.
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Other
(). Astrocytes influence medulloblastoma phenotypes and CD133 surface expression. PloS One
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Blocking
(). H3K36 Methylation and the Chromodomain Protein Eaf3 Are Required for Proper Cotranscriptional Spliceosome Assembly. Cell Rep.
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Undefined
(). RalA and RalB relocalization to depolarized mitochondria depends on clathrin-mediated endocytosis and facilitates TBK1 activation. PLoS One.
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FC, FACS, FLOW
(). Cross-regulation of defective endolysosome trafficking and enhanced autophagy through TFEB in UNC13D deficiency. Autophagy.
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IF, Confocal Microscopy
(). ZnO nanoparticles affect nutrient transport in an in vitro model of the small intestine. Food Chem Toxicol.
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IHC, ICC, Histology
(). Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation. J Vis Exp.
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Other
(). Staining of Frozen and Formalin-Fixed, Paraffin-Embedded Tissues with Metal-Labeled Antibodies for Imaging Mass Cytometry Analysis. Curr Protoc Cytom.
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IF, Confocal Microscopy
(). Differentiation of Myeloid-derived Suppressor Cells from Murine Bone Marrow and Their Co-culture with Splenic Dendritic Cells. Bio Protoc.
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(). A three dimensional in vitro glial scar model to investigate the local strain effects from micromotion around neural implants. Lab on a Chip
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Microarray, Microarray chip
(). Effects of delayed delivery of dexamethasone-21-phosphate via subcutaneous microdialysis implants on macrophage activation in rats. Acta Biomaterialia
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Blocking
(). Localized delivery of dexamethasone-21-phosphate via microdialysis implants in rat induces M(GC) macrophage polarization and alters CCL2 concentrations. Acta Biomaterialia
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Blocking
(). A detailed three-step protocol for live imaging of intracellular traffic in polarized primary porcine RPE monolayers. Experimental Eye Research
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Blocking; IF, Confocal Microscopy
(). Comparison of microdialysis sampling perfusion fluid components on the foreign body reaction in rat subcutaneous tissue. European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
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Buffer
(). Structural and functional insight into how the Plasmodium falciparum VAR2CSA protein mediates binding to chondroitin sulfate A in placental malaria. J Biol Chem.
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Other
(). Pre-targeting and direct immunotargeting of liposomal drug carriers to ovarian carcinoma. PloS One
Applications
Blocking
(). Quantitative detection of PfHRP2 in saliva of malaria patients in the Philippines Malar J.
Applications
EIA, ELISA
(). Antioxidant therapy attenuates myocardial telomerase activity reduction in superoxide dismutase-deficient mice. J Mol Cell Cardiol.
Applications
IHC, ICC, Histology
(). Studying cell rolling trajectories on asymmetric receptor patterns. J Vis Exp.
Applications
Other
(). The chondroitin sulfate A-binding site of the VAR2CSA protein involves multiple N-terminal domains. J Biol Chem.
Applications
Other
(). Examining the lateral displacement of HL60 cells rolling on asymmetric P-selectin patterns. Langmuir : the Acs Journal of Surfaces and Colloids
Applications
Control
(). Dynamic remodeling of subcellular chemical gradients using a multi-directional flow device. Lab Chip.
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Other
(). Chondroitin sulphate A (CSA)-binding of single recombinant Duffy-binding-like domains is not restricted to Plasmodium falciparum Erythrocyte Membrane Protein 1 expressed by CSA-binding parasites. Int J Parasitol.
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E, EIA
(). Weight increase and overweight are associated with DNA oxidative damage in skeletal muscle. Clin Nutr.
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IHC, ICC, Histology
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Other
(). Prototyping of microfluidic devices in poly (dimethylsiloxane) using solid-object printing. Anal Chem.
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IF, Confocal Microscopy

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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