Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-Adsorbed
Goat anti-Mouse mx Human HRP ELISA
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Datasheet

Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-Adsorbed

Goat Polyclonal

610-1319-0100 610-1319-0500 610-1319
100 µg 500 µg 2 mg
ELISA, IHC, Other, WB
Mouse
Goat
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$73.00 /Per Item
$109.00 /Per Item
$185.00 /Per Item
100 µg $73.00
500 µg $109.00
2 mg $185.00
Availability: Ships next business day Availability: Ships on approximately 05/08/2023 Availability: Ships next business day
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Description

Background

Anti-Mouse IgG Peroxidase Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Note

Mouse secondary antibody conjugated to peroxidase is available in a variety of formats. Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and is suitable for ELISA, Immunohistochemistry western blotting as well as other Horseradish Peroxidase HRP antibody based assays.

Purity/Specificity

HRP Mouse Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Human Serum Proteins.

Anti-MOUSE IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Human Serum Proteins) - 610-1319
Goat Anti-Mouse IgG Secondary Antibody Peroxidase Conjugated, Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated, GAM-HRP, Anti-mouse secondary antibody, anti-mouse HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-mouse horseradish peroxidase conjugated secondary antibody
ELISA
IHC, Other, WB
Mouse
Goat
IgG (H&L)
Peroxidase (Horseradish)
Polyclonal
IgG
Mouse IgG whole molecule
Lyophilized
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
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WB, IB, PCA
p53 isoforms differentially impact on the POLι dependent DNA damage tolerance pathway. Cell Death Dis. ()
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WB, IB, PCA
Phosphorylation of a Human Microprotein Promotes Dissociation of Biomolecular Condensates. J Am Chem Soc. ()
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WB, IB, PCA
Autophagic degradation of IRF3 induced by the small-molecule auranofin inhibits its transcriptional and proapoptotic activities. J Biol Chem. ()
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WB, IB, PCA
Metallothionein 3-Zinc Axis Suppresses Caspase-11 Inflammasome Activation and Impairs Antibacterial Immunity. Front Immunol. ()
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WB, IB, PCA
Global profiling of cellular substrates of human Dcp2. Biochemistry. ()
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Undefined
Muscle structural, energetic and functional benefits of endurance exercise training in sickle cell disease. Am J Hematol. ()
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E, EIA; Other
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Undefined
Discovery of cellular substrates of human RNA-decapping enzyme DCP2 using a stapled bicyclic peptide inhibitor. Cell Chem Biol. ()
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WB, IB, PCA
Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking. Cell Rep. ()
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WB, IB, PCA
Formation of mRNP granules in Toxoplasma gondii during the lytic cycle. Mol Biochem Parasitol. ()
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WB, IB, PCA
Distinct disruptions in Land's cycle remodeling of glycerophosphocholines in murine cortex mark symptomatic onset and progression in two Alzheimer's disease mouse models. J Neurochem. ()
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WB, IB, PCA
The 3' UTRs of Brain-Derived Neurotrophic Factor Transcripts Differentially Regulate the Dendritic Arbor. Frontiers in Cellular Neuroscience ()
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WB, IB, PCA
Toxoplasma ubiquitin-like protease 1, a key enzyme in sumoylation and desumoylation pathways, is under the control of non-coding RNAs. Int J Parasitol. ()
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WB, IB, PCA
A human microprotein that interacts with the mRNA decapping complex. Nature Chemical Biology ()
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WB, IB, PCA
Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels. PLOS One ()
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WB, IB, PCA
Activating transcription factor 3 attenuates chemokine and cytokine expression in mouse skeletal muscle after exercise and facilitates molecular adaptation to endurance training. FASEB J. ()
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WB, IB, PCA
Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells. Oncotarget. ()
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WB, IB, PCA
Bis (monoacylglycero) phosphate inhibits TLR4-dependent RANTES production in macrophages. Int J Biochem Cell Biol. ()
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Undefined
A TgCRND8 mouse model of Alzheimer's disease exhibits sexual dimorphisms in behavioral indices of cognitive reserve. J Alzheimers Dis. ()
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WB, IB, PCA
Utilization of inherent miRNAs in functional analyses of Toxoplasma gondii genes. J Microbiol Methods. ()
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WB, IB, PCA
Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA. Nature. ()
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WB, IB, PCA
Characterization of a homolog of DEAD-box RNA helicases in Toxoplasma gondii as a marker of cytoplasmic mRNP stress granules. Gene. ()
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WB, IB, PCA
Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research. Molecular Brain ()
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WB, IB, PCA
Phosphoproteome analysis of an early onset mouse model (T g CRND 8) of A lzheimer's disease reveals temporal changes in neuronal and glia signaling pathways. Proteomics. ()
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WB, IB, PCA
A molecular mechanism for TNF-α–mediated downregulation of B cell responses. J Immunol. ()
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Circulating microparticles carry a functional endothelial nitric oxide synthase that is decreased in patients with endothelial dysfunction. J Am Heart Assoc. ()
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WB, IB, PCA
Dihydroxybenzoic acid isomers differentially dissociate soluble biotinyl-Aβ(1-42) oligomers. Biochemistry ()
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E, EIA
Vaccination with different M2e epitope densities confers partial protection against H5N1 influenza A virus challenge in chickens. Intervirology. ()
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WB, IB, PCA
Localization and induction of the A2 virulence factor in Leishmania: evidence that A2 is a stress response protein. Mol Microbiol. ()
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WB, IB, PCA
Protein phosphatase 2A (PP2A) is increased in old murine B cells and mediates p38 MAPK/tristetraprolin dephosphorylation and E47 mRNA instability. Mech Ageing Dev. ()
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WB, IB, PCA
Proteolysis of submembrane cytoskeletal proteins ankyrin-G and αII-spectrin following diffuse brain injury: a role in white matter vulnerability at Nodes of Ranvier. Brain Pathology (Zurich, Switzerland) ()
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WB, IB, PCA
Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord. BMC Biotechnol. ()
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IHC, ICC, Histology
RNA stability of the E2A-encoded transcription factor E47 is lower in splenic activated B cells from aged mice. J Immunol. ()
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WB, IB, PCA
The polybasic juxtamembrane region of Sso1p is required for SNARE function in vivo. Eukaryot Cell. ()
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WB, IB, PCA
Reduced Ig class switch in aged mice correlates with decreased E47 and activation-induced cytidine deaminase. J Immunol. ()
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WB, IB, PCA
Age-related differences in the E2A-encoded transcription factor E47 in bone marrow-derived B cell precursors and in splenic B cells. Exp Gerontol. ()
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WB, IB, PCA
Effects of aging on proliferation and E47 transcription factor activity induced by different stimuli in murine splenic B cells. Mech Ageing Dev. ()
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WB, IB, PCA
The age-related decrease in E47 DNA-binding does not depend on increased Id inhibitory proteins in bone marrow-derived B cell precursors. Front Biosci. ()
Applications
WB, IB, PCA

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