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Datasheet

Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-Adsorbed

Goat Polyclonal

47 References
610-1319-0100 610-1319-0500 610-1319
100 µg 500 µg 2 mg
Lyophilized Lyophilized Lyophilized
WB, ELISA, IHC, Other
Mouse
Goat
$77.00 /Per Item
$115.00 /Per Item
$195.00 /Per Item
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Background

Anti-Mouse IgG Peroxidase Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Product Details

Goat Anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated (Min X Human Serum Proteins) - 610-1319
Goat Anti-Mouse IgG Secondary Antibody Peroxidase Conjugated, Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated, GAM-HRP, Anti-mouse secondary antibody, anti-mouse HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-mouse horseradish peroxidase conjugated secondary antibody
Goat
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG

Target Details

Mouse
Mouse IgG whole molecule
HRP Mouse Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Human Serum Proteins.

Application Details

ELISA
IHC, Other, WB  - View References
Mouse secondary antibody conjugated to peroxidase is available in a variety of formats. Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and is suitable for Immunohistochemistry, Western blotting as well as other antibody based assays.

Formulation

2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). Motor neuron activity enhances the proteomic stress caused by autophagy defects in the target muscle. PLoS One.
Applications
WB, IB, PCA
(). The Drosophila Nesprin-1 homolog MSP300 is required for muscle autophagy and proteostasis. J Cell Sci.
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WB, IB, PCA
(). DHA Supplementation during Pregnancy in Women with Obesity Normalizes IGF2R Levels in the Placenta of Male Newborns. Int J Endocrinol.
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WB, IB, PCA
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WB, IB, PCA
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WB, IB, PCA
(). Nascent alt-protein chemoproteomics reveals a pre-60S assembly checkpoint inhibitor. Nat Chem Biol.
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WB, IB, PCA
(). Mapping subcellular localizations of unannotated microproteins and alternative proteins with MicroID. Mol Cell.
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Undefined
(). CENP-A overexpression promotes aneuploidy with karyotypic heterogeneity. J Cell Biol.
Applications
WB, IB, PCA
(). p53 isoforms differentially impact on the POLι dependent DNA damage tolerance pathway. Cell Death Dis.
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WB, IB, PCA
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WB, IB, PCA
(). Autophagic degradation of IRF3 induced by the small-molecule auranofin inhibits its transcriptional and proapoptotic activities. J Biol Chem.
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WB, IB, PCA
(). Metallothionein 3-Zinc Axis Suppresses Caspase-11 Inflammasome Activation and Impairs Antibacterial Immunity. Front Immunol.
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WB, IB, PCA
(). Global profiling of cellular substrates of human Dcp2. Biochemistry.
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WB, IB, PCA
(). Muscle structural, energetic and functional benefits of endurance exercise training in sickle cell disease. Am J Hematol.
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WB, IB, PCA
(). Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C). Cell.
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Microneutralization Assay (MNT)
(). The NBDY microprotein regulates cellular RNA decapping. Biochemistry.
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WB, IB, PCA
(). Discovery of cellular substrates of human RNA-decapping enzyme DCP2 using a stapled bicyclic peptide inhibitor. Cell Chem Biol.
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WB, IB, PCA
(). Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking. Cell Rep.
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WB, IB, PCA
(). Formation of mRNP granules in Toxoplasma gondii during the lytic cycle. Mol Biochem Parasitol.
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WB, IB, PCA
(). Distinct disruptions in Land's cycle remodeling of glycerophosphocholines in murine cortex mark symptomatic onset and progression in two Alzheimer's disease mouse models. J Neurochem.
Applications
WB, IB, PCA
(). The 3' UTRs of Brain-Derived Neurotrophic Factor Transcripts Differentially Regulate the Dendritic Arbor. Frontiers in Cellular Neuroscience
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WB, IB, PCA
(). Toxoplasma ubiquitin-like protease 1, a key enzyme in sumoylation and desumoylation pathways, is under the control of non-coding RNAs. Int J Parasitol.
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WB, IB, PCA
(). A human microprotein that interacts with the mRNA decapping complex. Nature Chemical Biology
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WB, IB, PCA
(). Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels. PLOS One
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WB, IB, PCA
(). Activating transcription factor 3 attenuates chemokine and cytokine expression in mouse skeletal muscle after exercise and facilitates molecular adaptation to endurance training. FASEB J.
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WB, IB, PCA
(). Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells. Oncotarget.
Applications
WB, IB, PCA
(). Bis (monoacylglycero) phosphate inhibits TLR4-dependent RANTES production in macrophages. Int J Biochem Cell Biol.
Applications
EIA, ELISA
(). A TgCRND8 mouse model of Alzheimer's disease exhibits sexual dimorphisms in behavioral indices of cognitive reserve. J Alzheimers Dis.
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WB, IB, PCA
(). Utilization of inherent miRNAs in functional analyses of Toxoplasma gondii genes. J Microbiol Methods.
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WB, IB, PCA
(). Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA. Nature.
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WB, IB, PCA
(). Characterization of a homolog of DEAD-box RNA helicases in Toxoplasma gondii as a marker of cytoplasmic mRNP stress granules. Gene.
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WB, IB, PCA
(). Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research. Molecular Brain
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WB, IB, PCA
(). Phosphoproteome analysis of an early onset mouse model (T g CRND 8) of A lzheimer's disease reveals temporal changes in neuronal and glia signaling pathways. Proteomics.
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WB, IB, PCA
(). A molecular mechanism for TNF-α–mediated downregulation of B cell responses. J Immunol.
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WB, IB, PCA
(). Circulating microparticles carry a functional endothelial nitric oxide synthase that is decreased in patients with endothelial dysfunction. J Am Heart Assoc.
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WB, IB, PCA
(). Dihydroxybenzoic acid isomers differentially dissociate soluble biotinyl-Aβ(1-42) oligomers. Biochemistry
Applications
E, EIA
(). Vaccination with different M2e epitope densities confers partial protection against H5N1 influenza A virus challenge in chickens. Intervirology.
Applications
WB, IB, PCA
(). Localization and induction of the A2 virulence factor in Leishmania: evidence that A2 is a stress response protein. Mol Microbiol.
Applications
WB, IB, PCA
(). Protein phosphatase 2A (PP2A) is increased in old murine B cells and mediates p38 MAPK/tristetraprolin dephosphorylation and E47 mRNA instability. Mech Ageing Dev.
Applications
WB, IB, PCA
(). Proteolysis of submembrane cytoskeletal proteins ankyrin-G and αII-spectrin following diffuse brain injury: a role in white matter vulnerability at Nodes of Ranvier. Brain Pathology (Zurich, Switzerland)
Applications
WB, IB, PCA
(). Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord. BMC Biotechnol.
Applications
IHC, ICC, Histology
(). RNA stability of the E2A-encoded transcription factor E47 is lower in splenic activated B cells from aged mice. J Immunol.
Applications
WB, IB, PCA
(). The polybasic juxtamembrane region of Sso1p is required for SNARE function in vivo. Eukaryot Cell.
Applications
WB, IB, PCA
(). Reduced Ig class switch in aged mice correlates with decreased E47 and activation-induced cytidine deaminase. J Immunol.
Applications
WB, IB, PCA
(). Age-related differences in the E2A-encoded transcription factor E47 in bone marrow-derived B cell precursors and in splenic B cells. Exp Gerontol.
Applications
WB, IB, PCA
(). Effects of aging on proliferation and E47 transcription factor activity induced by different stimuli in murine splenic B cells. Mech Ageing Dev.
Applications
WB, IB, PCA
(). The age-related decrease in E47 DNA-binding does not depend on increased Id inhibitory proteins in bone marrow-derived B cell precursors. Front Biosci.
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.