Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
HRP conjugated Anti Rabbit IgG polyclonal antibody-Western blot
Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122)
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Datasheet

Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Goat Polyclonal

611-1322-0100 611-1322-0500 611-1322
100 µg 500 µg 2 mg
ELISA, WB, IHC
Rabbit
Goat
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$73.00 /Per Item
$109.00 /Per Item
$179.00 /Per Item
100 µg $73.00
500 µg $109.00
2 mg $179.00
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Description

Background

Anti-Rabbit IgG peroxidase conjugated antibody generated in goat detects specifically rabbit IgG. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG is conjugated with Peroxidase. This peroxidase conjugated anti-Rabbit secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Application Note

Goat Anti-Rabbit IgG peroxidase conjugated antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Purity/Specificity

Anti-RABBIT IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Human Serum Proteins.

Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Human Serum Proteins) - 611-1322
goat anti-Rabbit IgG Antibody peroxidase conjugated, Gt-a-Rabbit peroxidase conjugated IgG, Rabbit peroxidase conjugated Antibody in Goat, Rabbit Secondary peroxidase conjugated Antibody, HRP secondary, peroxidase secondary
ELISA, WB
IHC
Rabbit
Goat
IgG (H&L)
Peroxidase (Horseradish)
Polyclonal
IgG
Native Protein
Rabbit IgG whole molecule
Lyophilized
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
Chaperone mediated autophagy contributes to the newly synthesized histones H3 and H4 quality control. Nucleic Acids Res. ()
Applications
WB, IB, PCA
CENP-A overexpression promotes aneuploidy with karyotypic heterogeneity. J Cell Biol. ()
Applications
Undefined
p53 isoforms differentially impact on the POLι dependent DNA damage tolerance pathway. Cell Death Dis. ()
Applications
WB, IB, PCA
Autophagic degradation of IRF3 induced by the small-molecule auranofin inhibits its transcriptional and proapoptotic activities. J Biol Chem. ()
Applications
WB, IB, PCA
Endolysosomal Targeting of Mitochondria Is Integral to BAX-Mediated Mitochondrial Permeabilization during Apoptosis Signaling. Dev Cell. ()
Applications
Undefined
Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking. Cell Rep. ()
Applications
WB, IB, PCA
Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts? Animals (Basel). ()
Applications
IHC, ICC, Histology
Molecular features of steroid-binding antidins and their use for assaying serum progesterone. PLOS One ()
Applications
E, EIA
Spy1, a unique cell cycle regulator, alters viability in ALS motor neurons and cell lines in response to mutant SOD1-induced DNA damage. DNA Repair (Amst). ()
Applications
WB, IB, PCA
Toxoplasma gondii infection induces the formation of host's nuclear granules containing poly(A)-binding proteins. Can J Microbiol. ()
Applications
WB, IB, PCA
Docosahexaenoic acid inhibits cerulein-induced acute pancreatitis in rats. Nutrients. ()
Applications
IHC, ICC, Histology
Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells. Oncotarget. ()
Applications
WB, IB, PCA
Application of an E. coli signal sequence as a versatile inclusion body tag. Microbial Cell Factories ()
Applications
WB, IB, PCA
Electroacupuncture ameliorates learning and memory in rats with cerebral ischemia-reperfusion injury by inhibiting oxidative stress and promoting p-CREB expression in the hippocampus. Mol Med Rep. ()
Applications
WB, IB, PCA
Utilization of inherent miRNAs in functional analyses of Toxoplasma gondii genes. J Microbiol Methods. ()
Applications
WB, IB, PCA
Characterization of a homolog of DEAD-box RNA helicases in Toxoplasma gondii as a marker of cytoplasmic mRNP stress granules. Gene. ()
Applications
WB, IB, PCA
Sustained submicromolar H2O2 levels induce hepcidin via signal transducer and activator of transcription 3 (STAT3). J Biol Chem. ()
Applications
WB, IB, PCA
Apoptolysis: a novel mechanism of skin blistering in pemphigus vulgaris linking the apoptotic pathways to basal cell shrinkage and suprabasal acantholysis. Exp Dermatol. ()
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.