Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed
HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot
HRP-conjugated Goat-Anti-Rabbit secondary antibody- Immunoblot
ELISA Results of Goat Anti-Rabbit IgG Antibody Peroxidase Conjugated MX10
Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122)
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Datasheet

Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed

Goat Polyclonal

611-103-122
1 mg
Dot Blot, ELISA, WB, IHC
Rabbit
Goat
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$259.00 /Per Item
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Description

Background

Goat anti-Rabbit IgG (H&L) Antibody Conjugated to Horseradish Peroxidase (HRP) is designed for chemiluminescent detection. Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. To enable detection, the secondary antibody must have specificity for the antibody species and isotype of the primary antibody being used and generally is conjugated. This purified secondary antibody has been validated and optimized yielding good sensitivity and reproducible results with Rockland's primary antibodies. This anti-rabbit IgG antibody has been crossed adsorbed to show no reaction against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Application Note

Anti-Rabbit secondary antibody conjugated to horseradish peroxidase (HRP) generated in goat detects specifically rabbit IgG. This anti-rabbit HRP mx10 secondary antibody has been tested in ELISA, Dot Blot, and Western Blot. Although not tested, this product could be useful for Sandwich ELISA, titration assays, immunoprecipitation, immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Purity/Specificity

Conjugated Second Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) - 611-103-122
goat anti-rabbit hrp, HRP conjugated secondary antibody, goat anti-rabbit secondary antibody, goat anti-rabbit IgG peroxidase conjugated secondary
Dot Blot, ELISA, WB
IHC
Rabbit
Goat
IgG (H&L)
Peroxidase (Horseradish)
Polyclonal
IgG
Rabbit IgG whole molecule
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Alternative Hapten Design for Zearalenone Immunoreagent Generation. Toxins (Basel). ()
Applications
EIA, ELISA
Large-Scale Functionalized Metasurface-Based SARS-CoV-2 Detection and Quantification. ACS Nano, ()
Applications
WB, IB, PCA
Phosphorylation of a Human Microprotein Promotes Dissociation of Biomolecular Condensates. J Am Chem Soc. ()
Applications
WB, IB, PCA
Characterization of SARS-CoV-2 East Java isolate, Indonesia. F1000Res. ()
Applications
IHC, ICC, Histology; WB, IB, PCA
Calcium waves facilitate and coordinate the contraction of endfeet actin stress fibers in Drosophila interommatidial cells. Development. ()
Applications
WB, IB, PCA
Global profiling of cellular substrates of human Dcp2. Biochemistry. ()
Applications
Undefined
The NBDY microprotein regulates cellular RNA decapping. Biochemistry. ()
Applications
Undefined
Discovery of cellular substrates of human RNA-decapping enzyme DCP2 using a stapled bicyclic peptide inhibitor. Cell Chem Biol. ()
Applications
WB, IB, PCA
Pharmacological and genetic characterisation of the canine P2X4 receptor. Br J Pharmacol. ()
Applications
WB, IB, PCA
TET2 directs mammary luminal cell differentiation and endocrine response. Nat Commun. ()
Applications
WB, IB, PCA
Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts? Animals (Basel). ()
Applications
IHC, ICC, Histology
FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1. Oncotarget ()
Applications
WB, IB, PCA
A human microprotein that interacts with the mRNA decapping complex. Nature Chemical Biology ()
Applications
WB, IB, PCA
Neuronal CTGF/CCN2 negatively regulates myelination in a mouse model of tuberous sclerosis complex. Journal of Experimental Medicine ()
Applications
WB, IB, PCA
P2X7 antagonism using Brilliant Blue G reduces body weight loss and prolongs survival in female SOD1G93A amyotrophic lateral sclerosis mice. PeerJ ()
Applications
WB, IB, PCA
Docosahexaenoic acid inhibits cerulein-induced acute pancreatitis in rats. Nutrients. ()
Applications
IHC, ICC, Histology
Aminoguanidine exhibits an inhibitory effect on β‑amyloid‑induced damage in F98 glioma cells. Mol Med Rep. ()
Applications
WB, IB, PCA
Application of an E. coli signal sequence as a versatile inclusion body tag. Microbial Cell Factories ()
Applications
WB, IB, PCA
Simvastatin Ameliorates Matrix Stiffness-Mediated Endothelial Monolayer Disruption. PLOS One ()
Applications
WB, IB, PCA
The protein activator of protein kinase R, PACT/RAX, negatively regulates protein kinase R during mouse anterior pituitary development. The FEBS Journal ()
Applications
WB, IB, PCA
The Human dsRNA binding protein PACT is unable to functionally substitute for the Drosophila dsRNA binding protein R2D2. F1000Res. ()
Applications
WB, IB, PCA
Circulating microparticles carry a functional endothelial nitric oxide synthase that is decreased in patients with endothelial dysfunction. J Am Heart Assoc. ()
Applications
WB, IB, PCA
Aβ aggregation profiles and shifts in APP processing favor amyloidogenesis in canines. Neurobiology of Aging ()
Applications
WB, IB, PCA
APP(DeltaNL695) expression in murine tissue downregulates CNBP expression. Neuroscience Letters ()
Applications
WB, IB, PCA
Synergistic effects of long-term antioxidant diet and behavioral enrichment on beta-amyloid load and non-amyloidogenic processing in aged canines. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience ()
Applications
WB, IB, PCA
The effect of hypocapnia (PaCO2 27 mmHg) on CaM kinase IV activity, Bax/Bcl-2 protein expression and DNA fragmentation in the cerebral cortex of newborn piglets. Neurosci Lett. ()
Applications
Undefined
A chemical genetic screen for direct v-Src substrates reveals ordered assembly of a retrograde signaling pathway. Chem Biol. ()
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.