Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed
HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot
HRP-conjugated Goat-Anti-Rabbit secondary antibody- Immunoblot
ELISA Results of Goat Anti-Rabbit IgG Antibody Peroxidase Conjugated MX10
Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122)
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Datasheet

Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed

Goat Polyclonal

31 References
611-103-122
1 mg
WB, ELISA, IHC, Dot Blot
Rabbit
Goat
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Background

Goat anti-Rabbit IgG (H&L) Antibody Conjugated to Horseradish Peroxidase (HRP) is designed for chemiluminescent detection. Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. To enable detection, the secondary antibody must have specificity for the antibody species and isotype of the primary antibody being used and generally is conjugated. This purified secondary antibody has been validated and optimized yielding good sensitivity and reproducible results with Rockland's primary antibodies. This anti-rabbit IgG antibody has been crossed adsorbed to show no reaction against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Product Details

Goat Anti-Rabbit IgG (H&L) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) - 611-103-122
goat anti-rabbit hrp, HRP conjugated secondary antibody, goat anti-rabbit secondary antibody, goat anti-rabbit IgG peroxidase conjugated secondary
Goat
IgG (H&L)
Peroxidase (Horseradish)
Polyclonal
IgG

Target Details

Rabbit
Rabbit IgG whole molecule
Conjugated Second Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Application Details

Dot Blot, ELISA, WB
IHC  - View References
Anti-Rabbit secondary antibody conjugated to horseradish peroxidase (HRP) generated in goat detects specifically rabbit IgG. This anti-rabbit HRP mx10 secondary antibody has been tested in ELISA, Dot Blot, and Western Blot. Although not tested, this product could be useful for Sandwich ELISA, titration assays, immunoprecipitation, immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation

Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Matrix stiffness regulates tumor cell intravasation through expression and ESRP1-mediated alternative splicing of MENA. Cell Rep.
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WB, IB, PCA
(). Alternative Hapten Design for Zearalenone Immunoreagent Generation. Toxins (Basel).
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EIA, ELISA
(). Atorvastatin alleviates oxidative damage by activating the nuclear factor erythroid 2-related factor 2 pathway after brain ischemia in mice. Ann Transl Med.
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WB, IB, PCA
(). Diabetic hyperglycemia promotes primary tumor progression through glycation-induced tumor extracellular matrix stiffening. Sci Adv.
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(). Overexpressed angiotensin-converting enzyme in neutrophils suppresses glomerular damage in crescentic glomerulonephritis. Am J Physiol Renal Physiol.
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(). Large-Scale Functionalized Metasurface-Based SARS-CoV-2 Detection and Quantification. ACS Nano,
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WB, IB, PCA
(). Phosphorylation of a Human Microprotein Promotes Dissociation of Biomolecular Condensates. J Am Chem Soc.
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WB, IB, PCA
(). Characterization of SARS-CoV-2 East Java isolate, Indonesia. F1000Res.
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IHC, ICC, Histology; WB, IB, PCA
(). Calcium waves facilitate and coordinate the contraction of endfeet actin stress fibers in Drosophila interommatidial cells. Development.
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WB, IB, PCA
(). Global profiling of cellular substrates of human Dcp2. Biochemistry.
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WB, IB, PCA
(). The NBDY microprotein regulates cellular RNA decapping. Biochemistry.
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WB, IB, PCA
(). Discovery of cellular substrates of human RNA-decapping enzyme DCP2 using a stapled bicyclic peptide inhibitor. Cell Chem Biol.
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WB, IB, PCA
(). Pharmacological and genetic characterisation of the canine P2X4 receptor. Br J Pharmacol.
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(). TET2 directs mammary luminal cell differentiation and endocrine response. Nat Commun.
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WB, IB, PCA
(). Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts? Animals (Basel).
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IHC, ICC, Histology
(). FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1. Oncotarget
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WB, IB, PCA
(). A human microprotein that interacts with the mRNA decapping complex. Nature Chemical Biology
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WB, IB, PCA
(). Neuronal CTGF/CCN2 negatively regulates myelination in a mouse model of tuberous sclerosis complex. Journal of Experimental Medicine
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WB, IB, PCA
(). P2X7 antagonism using Brilliant Blue G reduces body weight loss and prolongs survival in female SOD1G93A amyotrophic lateral sclerosis mice. PeerJ
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WB, IB, PCA
(). Docosahexaenoic acid inhibits cerulein-induced acute pancreatitis in rats. Nutrients.
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IHC, ICC, Histology
(). Aminoguanidine exhibits an inhibitory effect on β‑amyloid‑induced damage in F98 glioma cells. Mol Med Rep.
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(). Application of an E. coli signal sequence as a versatile inclusion body tag. Microbial Cell Factories
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(). Simvastatin Ameliorates Matrix Stiffness-Mediated Endothelial Monolayer Disruption. PLOS One
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(). The protein activator of protein kinase R, PACT/RAX, negatively regulates protein kinase R during mouse anterior pituitary development. The FEBS Journal
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(). The Human dsRNA binding protein PACT is unable to functionally substitute for the Drosophila dsRNA binding protein R2D2. F1000Res.
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WB, IB, PCA
(). Circulating microparticles carry a functional endothelial nitric oxide synthase that is decreased in patients with endothelial dysfunction. J Am Heart Assoc.
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(). Aβ aggregation profiles and shifts in APP processing favor amyloidogenesis in canines. Neurobiology of Aging
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WB, IB, PCA
(). APP(DeltaNL695) expression in murine tissue downregulates CNBP expression. Neuroscience Letters
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(). Synergistic effects of long-term antioxidant diet and behavioral enrichment on beta-amyloid load and non-amyloidogenic processing in aged canines. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
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WB, IB, PCA
(). The effect of hypocapnia (PaCO2 27 mmHg) on CaM kinase IV activity, Bax/Bcl-2 protein expression and DNA fragmentation in the cerebral cortex of newborn piglets. Neurosci Lett.
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WB, IB, PCA
(). A chemical genetic screen for direct v-Src substrates reveals ordered assembly of a retrograde signaling pathway. Chem Biol.
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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.