Datasheet is currently unavailable. Try again or CONTACT US


1 mg
Dot Blot; ELISA
Have a Question? Ask a Specialist
$184.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.



Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, immunohistochemistry. Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).


HRP-SA, Horseradish Peroxidase conjugated S avidin, Streptococcus avidin conjugated to peroxidase, Streptavidin HRP, Streptavidin conjugated to horseradish peroxidase (HRP), HRP-linked Streptavidin

Application Note

Streptavidin Peroxidase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Blotting, and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).


Streptavidin-HRP was prepared from chromatographically purified streptavidin.  Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.

Storage Condition

Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Streptavidin Peroxidase conjugated is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Dot Blot
Peroxidase (Horseradish)
Expiration date is one (1) year from date of opening.
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Restore with deionized water (or equivalent)
1.0 mL

Invalid lot number

If you need help finding your CofA, contact us

  • Clinical relevance of nitrated beta 2-glycoprotein I in antiphospholipid syndrome: Implications for thrombosis risk.;2021;Krilis M et al.;PubMed
  • Double-label immunohistochemistry to assess labyrinth structure of the mouse placenta with stereology.;2020;De Clercq K. et al.;PubMed
  • Advanced maternal age compromises fetal growth and induces sex-specific changes in placental phenotype in rats.;2020;Napso T, Hung YP, Davidge ST, Care AS, Sferruzzi-Perri AN.;PubMed
  • Igf2 deletion alters mouse placenta endocrine capacity in a sexually dimorphic manner.;2020;Aykroyd BRL, Tunster SJ, Sferruzzi-Perri AN.;PubMed
  • The cysteine-free single mutant C32S of APEX2 is a highly expressed and active fusion tag for proximity labeling applications.;2019;Huang MS, Lin WC, Chang JH, Cheng CH, Wang HY, Mou KY.;PubMed
  • Self-Organization of Mouse Stem Cells into an Extended Potential Blastoid.;2019;Sozen B, Cox AL, De Jonghe J, et al.;PubMed
  • Fetal and trophoblast PI3K p110α have distinct roles in regulating resource supply to the growing fetus in mice.;2019;López-Tello J, Pérez-García V, Khaira J, et al.;PubMed
  • Polysialic Acid Modulates the Binding of External Lactoferrin in Neutrophil Extracellular Traps.;2019;Kühnle A et al.;PubMed
  • Maternal group 2 innate lymphoid cells control fetal growth and protect from endotoxin-induced abortion in mice.;2018;Elisa Balmas et al.;PubMed
  • Oleocanthal ameliorates amyloid-β oligomers' toxicity on astrocytes and neuronal cells: In vitro studies.;2017;Batarseh YS et al.;PubMed
  • Entry Sites of Venezuelan and Western Equine Encephalitis Viruses in the Mouse Central Nervous System following Peripheral Infection. ;2016;Phillips et al.;PubMed
  • A complement-microglial axis drives synapse loss during virus-induced memory impairment.;2016;Vasek MJ, Garber C, Dorsey D, et al.;PubMed
  • TREM2 regulates microglial cell activation in response to demyelination in vivo.;2015;Cantoni C, Bollman B, Licastro D, et al.;PubMed
  • A distinct subfraction of Aβ is responsible for the high-affinity Pittsburgh compound B-binding site in Alzheimer's disease brain.;2014;Matveev SV et al.;PubMed
  • Dihydroxybenzoic acid isomers differentially dissociate soluble biotinyl-Aβ(1-42) oligomers.;2012;LeVine H et al.;PubMed
  • The free and bound forms of Lpp occupy distinct subcellular locations in Escherichia coli.;2011;Cowles CE, Li Y, Semmelhack MF, Cristea IM, Silhavy TJ.;PubMed
  • Immobilization of growth factors on solid supports for the modulation of stem cell fate.;2010;Pompe T, Salchert K, Alberti K, Zandstra P, Werner C.;PubMed
  • Csf2 null mutation alters placental gene expression and trophoblast glycogen cell and giant cell abundance in mice.;2009;Sferruzzi-Perri AN, Macpherson AM, Roberts CT, Robertson SA.;PubMed