STREPTAVIDIN PEROXIDASE Conjugated
Peroxidase Conjugated Streptavidin - Western Blot
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Datasheet

STREPTAVIDIN PEROXIDASE Conjugated

S000-03
1 mg
Dot Blot, WB, ELISA, EM, IF, IHC
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$184.00 /Per Item
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Description

Background

Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, immunohistochemistry. Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).

Application Note

Streptavidin Peroxidase has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Blotting, and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).

Purity/Specificity

Streptavidin-HRP was prepared from chromatographically purified streptavidin.  Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.

Storage Condition

Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Streptavidin Peroxidase conjugated is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

STREPTAVIDIN PEROXIDASE Conjugated - S000-03
HRP-SA, Horseradish Peroxidase conjugated S avidin, Streptococcus avidin conjugated to peroxidase, Streptavidin HRP, Streptavidin conjugated to horseradish peroxidase (HRP), HRP-linked Streptavidin
Dot Blot, WB
ELISA, EM, IF, IHC
Peroxidase (Horseradish)
Lyophilized
Ambient
Expiration date is one (1) year from date of opening.
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Restore with deionized water (or equivalent)
1.0 mL
Napso T et al. Diet-induced maternal obesity impacts feto-placental growth and induces sex-specific alterations in placental morphology, mitochondrial bioenergetics, dynamics, lipid metabolism and oxidative stress in mice. (2022)
Applications
IHC, ICC, Histology
Aykroyd BRL et al. Loss of imprinting of the Igf2-H19 ICR1 enhances placental endocrine capacity via sex-specific alterations in signalling pathways in the mouse. (2022)
Applications
IHC, ICC, Histology
Sandovici I et al. The imprinted Igf2-Igf2r axis is critical for matching placental microvasculature expansion to fetal growth. (2022)
Applications
IHC, ICC, Histology
Krilis M et al. Clinical relevance of nitrated beta 2-glycoprotein I in antiphospholipid syndrome: Implications for thrombosis risk. (2021)
Applications
Undefined
Trujillo-Cenoz O et al. The ependymal cell cytoskeleton in the normal and injured spinal cord of mice. (2021)
Applications
Undefined
De Clercq K. et al. Double-label immunohistochemistry to assess labyrinth structure of the mouse placenta with stereology. (2020)
Applications
IHC, ICC, Histology
Napso T, Hung YP, Davidge ST, Care AS, Sferruzzi-Perri AN. Advanced maternal age compromises fetal growth and induces sex-specific changes in placental phenotype in rats. (2020)
Applications
IHC, ICC, Histology
Aykroyd BRL, Tunster SJ, Sferruzzi-Perri AN. Igf2 deletion alters mouse placenta endocrine capacity in a sexually dimorphic manner. (2020)
Applications
Undefined
Huang MS, Lin WC, Chang JH, Cheng CH, Wang HY, Mou KY. The cysteine-free single mutant C32S of APEX2 is a highly expressed and active fusion tag for proximity labeling applications. (2019)
Applications
WB, IB, PCA
Sozen B, Cox AL, De Jonghe J, et al. Self-Organization of Mouse Stem Cells into an Extended Potential Blastoid. (2019)
Applications
Undefined
López-Tello J, Pérez-García V, Khaira J, et al. Fetal and trophoblast PI3K p110α have distinct roles in regulating resource supply to the growing fetus in mice. (2019)
Applications
IF, Confocal Microscopy
Kühnle A et al. Polysialic Acid Modulates the Binding of External Lactoferrin in Neutrophil Extracellular Traps. (2019)
Applications
WB, IB, PCA
Elisa Balmas et al. Maternal group 2 innate lymphoid cells control fetal growth and protect from endotoxin-induced abortion in mice. (2018)
Applications
IHC, ICC, Histology
Batarseh YS et al. Oleocanthal ameliorates amyloid-β oligomers' toxicity on astrocytes and neuronal cells: In vitro studies. (2017)
Applications
E, EIA
Phillips et al. Entry Sites of Venezuelan and Western Equine Encephalitis Viruses in the Mouse Central Nervous System following Peripheral Infection. (2016)
Applications
IHC, ICC, Histology
Vasek MJ, Garber C, Dorsey D, et al. A complement-microglial axis drives synapse loss during virus-induced memory impairment. (2016)
Applications
Immuno-EM
Cantoni C, Bollman B, Licastro D, et al. TREM2 regulates microglial cell activation in response to demyelination in vivo. (2015)
Applications
Immuno-EM
Matveev SV et al. A distinct subfraction of Aβ is responsible for the high-affinity Pittsburgh compound B-binding site in Alzheimer's disease brain. (2014)
Applications
E, EIA
LeVine H et al. Dihydroxybenzoic acid isomers differentially dissociate soluble biotinyl-Aβ(1-42) oligomers. (2012)
Applications
E, EIA
Cowles CE, Li Y, Semmelhack MF, Cristea IM, Silhavy TJ. The free and bound forms of Lpp occupy distinct subcellular locations in Escherichia coli. (2011)
Applications
WB, IB, PCA
Pompe T, Salchert K, Alberti K, Zandstra P, Werner C. Immobilization of growth factors on solid supports for the modulation of stem cell fate. (2010)
Applications
E, EIA
Sferruzzi-Perri AN, Macpherson AM, Roberts CT, Robertson SA. Csf2 null mutation alters placental gene expression and trophoblast glycogen cell and giant cell abundance in mice. (2009)
Applications
IHC, ICC, Histology

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.