STREPTAVIDIN ALKALINE PHOSPHATASE Conjugated
Western Blot - STREPTAVIDIN ALKALINE PHOSPHATASE Conjugated
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Datasheet

STREPTAVIDIN ALKALINE PHOSPHATASE Conjugated

S000-05
1 mg
ELISA, WB
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$214.00 /Per Item
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Description

Background

Streptavidin is a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's affinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substrate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments.

Application Note

Streptavidin Alkaline Phosphatase conjugated has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (NPP-10).

Purity/Specificity

Streptavidin-Alkaline Phosphatase was prepared from electrophoretically pure Streptavidin.   Alkaline Phosphatase conjugated Streptavidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Streptavidin.

STREPTAVIDIN ALKALINE PHOSPHATASE Conjugated - S000-05
SA alkaline phosphatase conjugate, S avidin conjugated to alkaline phosphatase, alkaline phosphatase conjugated to streptococcus avidin, streptavidin Alk Phos, SA-ALP, ALP conjugated streptavidin
ELISA, WB
Alkaline Phosphatase (Calf Intestine)
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
0.05% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Wet Ice
Store vial at 4° C before opening. DO NOT FREEZE. Streptavidin Alkaline Phosphatase conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Expiration date is one (1) year from date of opening.
Law, ME et al. Inhibitors of ERp44, PDIA1, and AGR2 induce disulfide-mediated oligomerization of Death Receptors 4 and 5 and cancer cell death. Cancer Letters (2022)
Applications
WB, IB, PCA
Tham, M et al. Macrophage depletion reduces postsurgical tumor recurrence and metastatic growth in a spontaneous murine model of melanoma. Oncotarget (2015)
Applications
IHC, ICC, Histology
Edwards KA, Baeumner AJ. Periplasmic binding protein-based detection of maltose using liposomes: a new class of biorecognition elements in competitive assays. Anal Chem. (2013)
Applications
E, EIA
Kiening M et al. Microplate-based screening methods for the efficient development of sandwich immunoassays. Analyst. (2005)
Applications
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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.