Streptavidin Cy3 Conjugated
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Datasheet

Streptavidin Cy3 Conjugated

S000-04
1 mg
Dot Blot, IF
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$192.00 /Per Item
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Description

Background

Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, immunohistochemistry. Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Rockland conjugates a broad group of secondary antibodies to many of the classic fluorescent markers including fluorescein, rhodamine, Texas Red, CyDyes™ and Phycoerythrin (RPE). All of the conjugates are ideal for various immunofluorescence based assays including fluorescent western blotting, immunofluorescence microscopy, FLISA, and more. Rockland also produces many next generation fluorochrome dyes designed for detection of primary antibodies in multiplex, multi-color analysis.

Application Note

Streptavidin CY3 conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purity/Specificity

This product was prepared from chromatographically pure Streptavidin.   Assay by immuno-electrophoresis resulted in a single precipitin arc against anti-Streptavidin.

STREPTAVIDIN CY3 Conjugated - S000-04
SA, S avidin, streptococcus avidin, Streptavidin Cy3 Conjugated
Dot Blot
IF
Cy3™
4.1 moles CY3 per mole of
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Thiesler H et al. Polysialic acid and polysialic acid receptors as regulators of microglia and macrophage activation. Cell Mol Life Sci. (2021)
Applications
IHC, ICC, Histology
Lui BG, Salomon N, Wüstehube-Lausch J, et al. Targeting the tumor vasculature with engineered cystine-knot miniproteins. Nat Commun. (2020)
Applications
IF, Confocal Microscopy
Abeln M et al. Sialic acid is a critical fetal defense against maternal complement attack. J Clin Invest. (2019)
Applications
IF, Confocal Microscopy; IHC, ICC, Histology
Van Sluis et al. A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage. Genes & Development (2015)
Applications
IF, Confocal Microscopy
Shen, CI et al. The infection of chicken tracheal epithelial cells with a H6N1 avian influenza virus. PloS One (2011)
Applications
IHC, ICC, Histology
Fessing, MY et al. p63 regulates Satb1 to control tissue-specific chromatin remodeling during development of the epidermis. The Journal of Cell Biology (2011)
Applications
IF, Confocal Microscopy
Huggenberger, R. et al. Stimulation of lymphangiogenesis via VEGFR-3 inhibits chronic skin inflammation. The Journal of Experimental Medicine (2010)
Applications
IHC, ICC, Histology
Sow, FB. et al. Laser Capture Microdissection Revisited as a Tool for Transcriptomic Analysis: Application of an Excel-Based qPCR Preparation Software (PREXCEL-Q). International Journal of Biomedical Science : Ijbs (2009)
Applications
IHC, ICC, Histology
Gallup, JM. et al. New quick method for isolating RNA from laser captured cells stained by immunofluorescent immunohistochemistry; RNA suitable for direct use in fluorogenic TaqMan one-step real-time RT-PCR. Biological Procedures Online (2005)
Applications
IHC, ICC, Histology; IF, Confocal Microscopy

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.