Streptavidin Fluorescein Conjugated
Dot Blot of STREPTAVIDIN Fluorescein Conjugated.
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Datasheet

Streptavidin Fluorescein Conjugated

S000-02
1 mg
Dot Blot, FC, IF
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$153.00 /Per Item
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Description

Background

Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, immunohistochemistry. Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Rockland conjugates a broad group of secondary antibodies to many of the classic fluorescent markers including fluorescein, rhodamine, Texas Red, CyDyes™ and Phycoerythrin (RPE). All of the conjugates are ideal for various immunofluorescence based assays including fluorescent western blotting, immunofluorescence microscopy, FLISA, and more. Rockland also produces many next generation fluorochrome dyes designed for detection of primary antibodies in multiplex, multi-color analysis.

Application Note

Streptavidin Fluorescein Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purity/Specificity

This product was prepared from chromatographically pure Streptavidin.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Streptavidin.

STREPTAVIDIN Fluorescein Conjugated - S000-02
SA, S avidin, streptococcus avidin, streptavidin FITC
Dot Blot
FC, IF
Fluorescein (FITC)
2.2 moles FITC per mole of
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Poltavets, V et al. CXCR4-CCR7 Heterodimerization Is a Driver of Breast Cancer Progression. Life (Basel, Switzerland) (2021)
Applications
FC, FACS, FLOW
Mertinková P, et. al. A simple and rapid pipeline for identification of receptor-binding sites on the surface proteins of pathogens. Sci Rep. (2020)
Applications
IF, Confocal Microscopy
Huang MS, Lin WC, Chang JH, Cheng CH, Wang HY, Mou KY. The cysteine-free single mutant C32S of APEX2 is a highly expressed and active fusion tag for proximity labeling applications. Protein Sci. (2019)
Applications
FC, FACS, FLOW
Vitacolonna M et al. In-vivo quantification of the revascularization of a human acellular dermis seeded with EPCs and MSCs in co-culture with fibroblasts and pericytes in the dorsal chamber model in pre-irradiated tissue. Cell Tissue Bank. (2017)
Applications
IF, Confocal Microscopy
Shen, Z et al. Helicobacter cinaedi induced typhlocolitis in Rag-2-deficient mice. Helicobacter (2015)
Applications
IF, Confocal Microscopy
Patterson, KG et al. Control of established colon cancer xenografts using a novel humanized single chain antibody-streptococcal superantigen fusion protein targeting the 5T4 oncofetal antigen. PloS One (2014)
Applications
FC, FACS, FLOW
Möllmann, H. et al. Stem cell-mediated natural tissue engineering. Journal of Cellular and Molecular Medicine (2011)
Applications
IHC, ICC, Histology

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.