Background

Anti-Rabbit IgG Antibody peroxidase generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to peroxidase.

Product Details

Goat Anti-Rabbit IgG (H&L) Antibody Peroxidase Conjugated - 611-1302
Goat anti-Rabbit IgG Secondary Antibody Peroxidase Conjugated, Goat anti-Rabbit IgG Secondary Antibody HRP Conjugated, GAR-HRP, Anti-rabbit secondary antibody, anti-rabbit HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-rabbit horseradish peroxidase conjugated secondary antibody
Goat
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG

Target Details

Rabbit
Rabbit IgG whole molecule
RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Application Details

ELISA, WB
IHC  - View References
Anti-Rabbit IgG Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation

2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Mitochondrial H2O2 release does not directly cause damage to chromosomal DNA. Nat Commun.
Applications
WB, IB, PCA
(). Suppressive Activity of Boiogito, a Japanese Traditional Kampo Medicine, on Periostin Secretion in Human Fibroblast-Like Synoviocytes In Vitro. Cureus.
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WB, IB, PCA
(). Functional assessment of a novel biallelic MYH3 variation causing CPSKF1B (contractures, pterygia, and spondylocarpotarsal fusion syndrome1B). Mol Genet Genomic Med.
Applications
WB, IB, PCA
(). Bacillus subtilis YisK possesses oxaloacetate decarboxylase activity and exhibits Mbl-dependent localization. J Bacteriol.
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WB, IB, PCA
(). Diacylglycerol kinase-ε is S-palmitoylated on cysteine in the cytoplasmic end of its N-terminal transmembrane fragment. J Lipid Res.
Applications
WB, IB, PCA
(). Cardiomyocytes, cardiac endothelial cells and fibroblasts contribute to anthracycline-induced cardiac injury through RAS-homologous small GTPases RAC1 and CDC42. Pharmacol Res.
Applications
WB, IB, PCA
(). Co-culture models of endothelial cells, macrophages, and vascular smooth muscle cells for the study of the natural history of atherosclerosis. PLoS One.
Applications
WB, IB, PCA
(). Neurogenin 2 and Neuronal Differentiation 1 Control Proper Development of the Chick Trigeminal Ganglion and Its Nerve Branches. J Dev Biol.
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WB, IB, PCA
(). Massively parallel knock-in engineering of human T cells. Nat Biotechnol.
Applications
Undefined
(). Effect of genetic polymorphisms rs2301113 and rs2057482 in the expression of HIF-1α protein in periodontal ligament fibroblasts subjected to compressive force. JAppl Oral Sci.
Applications
WB, IB, PCA
(). Effects of Anticoccidial Vaccination and Taraxacum officinale Extract on the Growth Performance, Biochemical Parameters, Immunity, and Intestinal Morphology of Eimeria-Challenged Chickens. Life (Basel).
Applications
IHC, ICC, Histology
(). Reconstitution of a minimal ESX-5 type VII secretion system suggests a role for PPE proteins in the outer membrane transport of proteins. mSphere.
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WB, IB, PCA
(). Dual IKZF2 and CK1α degrader targets acute myeloid leukemia cells. Cancer Cell.
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WB, IB, PCA
(). Time-dependent homeostatic mechanisms underlie brain-derived neurotrophic factor action on neural circuitry. Commun Biol.
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WB, IB, PCA
(). DHA Supplementation during Pregnancy in Women with Obesity Normalizes IGF2R Levels in the Placenta of Male Newborns. Int J Endocrinol.
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WB, IB, PCA
(). Unannotated microprotein EMBOW regulates the interactome and chromatin and mitotic functions of WDR5. Cell Rep.
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WB, IB, PCA
(). Extracellular vesicles from gastric epithelial GES-1 cells infected with Helicobacter pylori promote changes in recipient cells associated with malignancy. Front Oncol.
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WB, IB, PCA
(). Alternative Hapten Design for Zearalenone Immunoreagent Generation. Toxins (Basel).
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EIA, ELISA
(). Neural crest cell-placodal neuron interactions are mediated by Cadherin-7 and N-cadherin during early chick trigeminal ganglion assembly. F1000Res.
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WB, IB, PCA
(). Nascent alt-protein chemoproteomics reveals a pre-60S assembly checkpoint inhibitor. Nat Chem Biol.
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WB, IB, PCA
(). Mapping subcellular localizations of unannotated microproteins and alternative proteins with MicroID. Mol Cell.
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Undefined
(). Palmitoyl‑RGD promotes the expression of dermal‑epidermal junction components in HaCaT cells. Mol Med Rep.
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WB, IB, PCA
(). Effects of hypoxia on antigen presentation and T cell-based immune recognition of HPV16-transformed cells. Front Immunol.
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WB, IB, PCA
(). Impact of Melatonin on RAW264. 7 Macrophages during Mechanical Strain. Int J Mol Sci.
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WB, IB, PCA
(). Impact of phosphorylation of heat shock protein 27 on the expression profile of periodontal ligament fibroblasts during mechanical strain. J Orofac Orthop.
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WB, IB, PCA
(). Large-Scale Functionalized Metasurface-Based SARS-CoV-2 Detection and Quantification. ACS Nano,
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WB, IB, PCA
(). Impact of melatonin on periodontal ligament fibroblasts during mechanical strain. Eur J Orthod.
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WB, IB, PCA
(). Impact of Leptin on the Expression Profile of Macrophages during Mechanical Strain In Vitro. Int J Mol Sci.
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WB, IB, PCA
(). Impact of salt and the osmoprotective transcription factor NFAT‐5 on macrophages during mechanical strain. Immunol Cell Biol.
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WB, IB, PCA
(). Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity. Int J Mol Sci.
Applications
WB, IB, PCA
(). Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model. J Clin Med.
Applications
IHC, ICC, Histology
(). Human-Relevant Sensitivity of iPSC-Derived Human Motor Neurons to BoNT/A1 and B1. Toxins (Basel).
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WB, IB, PCA
(). Modern Hybrid Rye, as an Alternative Energy Source for Broiler Chickens, Improves the Absorption Surface of the Small Intestine Depending on the Intestinal Part and Xylanase Supplementation. Animals (Basel).
Applications
IHC, ICC, Histology; WB, IB, PCA
(). Truncation of the TPR domain of OGT alters substrate and glycosite selection. Anal Bioanal Chem.
Applications
WB, IB, PCA
(). The effect of cellular and physiological indicators on gender determination. Cell Mol Biol (Noisy-le-grand).
Applications
WB, IB, PCA
(). Effects of mechanical strain on periodontal ligament fibroblasts in presence of Aggregatibacter actinomycetemcomitans lysate. BMC Oral Health.
Applications
WB, IB, PCA
(). Analgesic effect of voluntary exercise in a rat model of persistent pain via suppression of microglial activation in the spinal cord. Biomed Res.
Applications
WB, IB, PCA
(). Analgesic Effect of Combined Therapy with the Japanese Herbal Medicine “Yokukansan” and Electroacupuncture in Rats with Acute Inflammatory Pain. Medicines (Basel).
Applications
WB, IB, PCA
(). Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale. Nat Commun.
Applications
WB, IB, PCA
(). Impact of Leptin on Periodontal Ligament Fibroblasts during Mechanical Strain. Int J Mol Sci.
Applications
WB, IB, PCA
(). Effects of histamine on human periodontal ligament fibroblasts under simulated orthodontic pressure. PLoS One.
Applications
WB, IB, PCA
(). Role of Oxygen Supply in Macrophages in a Model of Simulated Orthodontic Tooth Movement. Mediators Inflamm.
Applications
WB, IB, PCA
(). Low Density Lipoprotein Receptor-Related Protein-1 (LRP1) Is Involved in the Uptake of Clostridioides difficile Toxin A and Serves as an Internalizing Receptor. Front Cell Infect Microbiol.
Applications
WB, IB, PCA
(). Role and Regulation of Mechanotransductive HIF-1α Stabilisation in Periodontal Ligament Fibroblasts. Int J Mol Sci.
Applications
WB, IB, PCA
(). Regulation of Mitochondrial Respiratory Chain Complex Levels, Organization, and Function by Arginyltransferase 1. Front Cell Dev Biol
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WB, IB, PCA
(). Distinct roles of mitochondrial HIGD1A and HIGD2A in respiratory complex and supercomplex biogenesis. Cell Rep.
Applications
WB, IB, PCA
(). Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis. STAR Protoc.
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WB, IB, PCA
(). Relative contributions of the cGAS-STING and TLR3 signaling pathways to attenuation of herpes simplex virus 1 replication. J Virol.
Applications
WB, IB, PCA
(). FHOD-1 is the only formin in Caenorhabditis elegans that promotes striated muscle growth and Z-line organization in a cell autonomous manner. Cytoskeleton (Hoboken).
Applications
WB, IB, PCA
(). Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts? Animals (Basel).
Applications
IHC, ICC, Histology
(). Engineering a Proximity-Directed O-GlcNAc Transferase for Selective Protein O-GlcNAcylation in Cells. ACS Chem Biol.
Applications
WB, IB, PCA
(). Difference in Mono-O-Glucosylation of Ras Subtype GTPases Between Toxin A and Toxin B From Clostridioides difficile Strain 10463 and Lethal Toxin From Clostridium sordellii Strain 6018. Frontiers in Microbiology
Applications
WB, IB, PCA
(). Feeding cycle alters the biophysics and molecular expression of voltage-gated Na+ currents in rat hippocampal CA1 neurones. Eur J Neurosci.
Applications
WB, IB, PCA
(). Preliminary study on the role of miR‑148a and DNMT1 in the pathogenesis of acute myeloid leukemia. Mol Med Rep.
Applications
WB, IB, PCA
(). Distinct contribution of Rac1 expression in cardiomyocytes to anthracycline-induced cardiac injury. Biochem Pharmacol.
Applications
WB, IB, PCA
(). Nutrient availability shapes methionine metabolism in p16/MTAP-deleted cells. Sci Adv.
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WB, IB, PCA
(). Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841). Mol Med Rep.
Applications
WB, IB, PCA
(). Small Molecule Interactome Mapping by Photo-Affinity Labeling (SIM-PAL) to Identify Binding Sites of Small Molecules on a Proteome-Wide Scale. Curr Protoc Chem Biol.
Applications
WB, IB, PCA
(). Osteoprotective action of low-salt diet requires myeloid cell-derived NFAT5. JCI Insight.
Applications
WB, IB, PCA
(). Adeno-associated virus serotype 9 mediated vascular endothelial growth factor gene overexpression in mdx mice. Experimental and Therapeutic Medicine
Applications
WB, IB, PCA
(). Thymoquinone Attenuates Cardiomyopathy in Streptozotocin-Treated Diabetic Rats. Oxidative Medicine and Cellular Longevity
Applications
WB, IB, PCA
(). Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B. Frontiers in Microbiology
Applications
WB, IB, PCA
(). Corticotropin-releasing hormone-binding protein is up-regulated by brain-derived neurotrophic factor and is secreted in an activity-dependent manner in rat cerebral cortical neurons. J Neurochem.
Applications
WB, IB, PCA
(). The Hcp-like protein HilE inhibits homodimerization and DNA binding of the virulence-associated transcriptional regulator HilD in Salmonella. J Biol Chem.
Applications
WB, IB, PCA
(). Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics. Cell Rep.
Applications
WB, IB, PCA
(). IFN-γ, IL-17A, or zonulin rapidly increase the permeability of the blood-brain and small intestinal epithelial barriers: Relevance for neuro-inflammatory diseases. Biochem Biophys Res Commun.
Applications
WB, IB, PCA
(). Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels. PLOS One
Applications
WB, IB, PCA
(). Identification of a substrate domain that determines system specificity in mycobacterial type VII secretion systems. Scientific Reports
Applications
WB, IB, PCA
(). TARP γ-2 Is Required for Inflammation-Associated AMPA Receptor Plasticity within Lamina II of the Spinal Cord Dorsal Horn. The Journal of Neuroscience
Applications
WB, IB, PCA
(). Increased bone morphogenetic protein-6 in follicular fluid and granulosa cells may correlate with fertilization and embryo quality in humans. Experimental and Therapeutic Medicine
Applications
WB, IB, PCA
(). The Rho ADP-ribosylating C3 exoenzyme binds cells via an Arg-Gly-Asp motif. Journal of Biological Chemistry
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WB, IB, PCA
(). A Predictive Model for Selective Targeting of the Warburg Effect through GAPDH Inhibition with a Natural Product. Cell Metabolism
Applications
WB, IB, PCA
(). Docosahexaenoic acid inhibits cerulein-induced acute pancreatitis in rats. Nutrients.
Applications
IHC, ICC, Histology
(). Single synonymous mutation in factor IX alters protein properties and underlies haemophilia B. J Med Genet.
Applications
E, EIA; WB, IB, PCA
(). New immunomodulatory role of neuropeptide Y (NPY) in Salmo salar leucocytes. Dev Comp Immunol.
Applications
WB, IB, PCA
(). S100P is associated with proliferation and migration in nasopharyngeal carcinoma. Oncol Lett.
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WB, IB, PCA
(). Application of an E. coli signal sequence as a versatile inclusion body tag. Microbial Cell Factories
Applications
WB, IB, PCA
(). Interleukin-17 potently increases non-small cell lung cancer growth. Molecular Medicine Reports
Applications
WB, IB, PCA
(). Paris Saponins enhance radiosensitivity in a gefitinib-resistant lung adenocarcinoma cell line by inducing apoptosis and G2/M cell cycle phase arrest. Molecular Medicine Reports
Applications
WB, IB, PCA
(). Elevated expression of chemokine C-C ligand 2 in stroma is associated with recurrent basal-like breast cancers. Modern Pathology
Applications
E, EIA; IHC, ICC, Histology; WB, IB, PCA
(). Role of p38alpha/beta MAP Kinase in Cell Susceptibility to Clostridium sordellii Lethal Toxin and Clostridium difficile Toxin B. Toxins
Applications
WB, IB, PCA
(). Endoplasmic reticulum stress-independent activation of unfolded protein response kinases by a small molecule ATP-mimic. Elife
Applications
WB, IB, PCA
(). TGF-β Negatively Regulates CXCL1 Chemokine Expression in Mammary Fibroblasts through Enhancement of Smad2/3 and Suppression of HGF/c-Met Signaling Mechanisms. PLOS One
Applications
WB, IB, PCA
(). Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling. The Journal of Neuroscience
Applications
WB, IB, PCA
(). Expression of glutathione peroxidase 1 in the spheno-occipital synchondrosis and its role in ROS-induced apoptosis. Eur J Orthod.
Applications
IHC, ICC, Histology
(). Identification of a collagen type I adhesin of Bacteroides fragilis. PLoS One.
Applications
WB, IB, PCA
(). The metal transporter SMF-3/DMT-1 mediates aluminum-induced dopamine neuron degeneration. J Neurochem.
Applications
WB, IB, PCA
(). Constitutive CCND1/CDK2 activity substitutes for p53 loss, or MYC or oncogenic RAS expression in the transformation of human mammary epithelial cells. PLoS One.
Applications
WB, IB, PCA
(). The Nrf2/SKN-1-dependent glutathione S-transferase π homologue GST-1 inhibits dopamine neuron degeneration in a Caenorhabditis elegans model of manganism. Neurotoxicology
Applications
WB, IB, PCA
(). Exploring leukocyte O-GlcNAcylation as a novel diagnostic tool for the earlier detection of type 2 diabetes mellitus. J Clin Endocrinol Metab.
Applications
WB, IB, PCA
(). Circulating microparticles carry a functional endothelial nitric oxide synthase that is decreased in patients with endothelial dysfunction. J Am Heart Assoc.
Applications
WB, IB, PCA
(). Aβ aggregation profiles and shifts in APP processing favor amyloidogenesis in canines. Neurobiology of Aging
Applications
WB, IB, PCA
(). TRAPPC9 mediates the interaction between p150 and COPII vesicles at the target membrane. PLoS One.
Applications
WB, IB, PCA
(). Matrix metalloproteinase-1 expression enhances tumorigenicity as well as tumor-related angiogenesis and is inversely associated with TIMP-4 expression in a model of glioblastoma. J Neurooncol.
Applications
WB, IB, PCA
(). Identification and characterization of Huntington related pathology: an in vivo DKI imaging study. Neuroimage
Applications
WB, IB, PCA
(). Specific chaperones for the type VII protein secretion pathway. J Biol Chem.
Applications
WB, IB, PCA
(). Annexin A6 Modulates Chick Cranial Neural Crest Cell Emigration. PLoS One.
Applications
WB, IB, PCA
(). Conserved Pro-Glu (PE) and Pro-Pro-Glu (PPE) protein domains target LipY lipases of pathogenic mycobacteria to the cell surface via the ESX-5 pathway. J Biol Chem.
Applications
WB, IB, PCA
(). Identification and characterization of PshBII, a second FA/FB-containing polypeptide in the photosynthetic reaction center of Heliobacterium modesticaldum. Photosynth Res.
Applications
WB, IB, PCA
(). APP(DeltaNL695) expression in murine tissue downregulates CNBP expression. Neuroscience Letters
Applications
WB, IB, PCA
(). Synergistic effects of long-term antioxidant diet and behavioral enrichment on beta-amyloid load and non-amyloidogenic processing in aged canines. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Applications
WB, IB, PCA
(). Induction of matrix metalloproteinase-1 and glioma cell motility by nitric oxide. J Neurooncol.
Applications
WB, IB, PCA
(). Positions of Trp codons in the leader peptide-coding region of the at operon influence anti-trap synthesis and trp operon expression in Bacillus licheniformis. J Bacteriol.
Applications
WB, IB, PCA
(). SKN-1/Nrf2 inhibits dopamine neuron degeneration in a Caenorhabditis elegans model of methylmercury toxicity. Toxicol Sci.
Applications
WB, IB, PCA
(). Expression of NKCC2 in the rat gastrointestinal tract. Neurogastroenterol Motil.
Applications
WB, IB, PCA; IHC, ICC, Histology
(). Chapter Twenty‐One Autophagy in Candida albicans. Methods in Enzymology
Applications
WB, IB, PCA
(). STAT3 activation inhibits human bronchial epithelial cell apoptosis in response to cigarette smoke exposure. Biochem Biophys Res Commun.
Applications
EIA, ELISA
(). The CC chemokine ligand 2 (CCL2) mediates fibroblast survival through IL-6. Am J Respir Cell Mol Biol.
Applications
E, EIA; WB, IB, PCA
(). Bax regulates c-Myc-induced mammary tumour apoptosis but not proliferation in MMTV-c-myc transgenic mice. Br J Cancer.
Applications
WB, IB, PCA
(). Development of a sensitive multi-well colorimetric assay for active NFkappaB. Nucleic Acids Res.
Applications
Other

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