Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated
Western Blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody
Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n 611-1102)
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Datasheet

Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated

Goat Polyclonal

611-1302
2 mg
ELISA, WB, IHC
Rabbit
Goat
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Description

Background

Anti-Rabbit IgG Antibody peroxidase generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to peroxidase.

Application Note

Anti-Rabbit IgG Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Purity/Specificity

RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
Goat anti-Rabbit IgG Secondary Antibody Peroxidase Conjugated, Goat anti-Rabbit IgG Secondary Antibody HRP Conjugated, GAR-HRP, Anti-rabbit secondary antibody, anti-rabbit HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-rabbit horseradish peroxidase conjugated secondary antibody
ELISA, WB
IHC
Rabbit
Goat
IgG (H&L)
Peroxidase (Horseradish)
Polyclonal
IgG
Rabbit IgG whole molecule
Lyophilized
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Schroder A et al. Impact of salt and the osmoprotective transcription factor NFAT‐5 on macrophages during mechanical strain. Immunol Cell Biol. (2021)
Applications
WB, IB, PCA
Schroder A et al. Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity. Int J Mol Sci. (2021)
Applications
WB, IB, PCA
Puzio I et al. Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model. J Clin Med. (2021)
Applications
IHC, ICC, Histology
Schenke M et al. Human-Relevant Sensitivity of iPSC-Derived Human Motor Neurons to BoNT/A1 and B1. Toxins (Basel). (2021)
Applications
WB, IB, PCA
Donaldson J et al. Modern Hybrid Rye, as an Alternative Energy Source for Broiler Chickens, Improves the Absorption Surface of the Small Intestine Depending on the Intestinal Part and Xylanase Supplementation. Animals (Basel). (2021)
Applications
IHC, ICC, Histology; WB, IB, PCA
Ramirez DH et al. Truncation of the TPR domain of OGT alters substrate and glycosite selection. Anal Bioanal Chem. (2021)
Applications
WB, IB, PCA
Haje MI et al. The effect of cellular and physiological indicators on gender determination. Cell Mol Biol (Noisy-le-grand). (2021)
Applications
WB, IB, PCA
Schroder A et al. Effects of mechanical strain on periodontal ligament fibroblasts in presence of Aggregatibacter actinomycetemcomitans lysate. BMC Oral Health. (2021)
Applications
WB, IB, PCA
Takahashi H et al. Analgesic effect of voluntary exercise in a rat model of persistent pain via suppression of microglial activation in the spinal cord. Biomed Res. (2021)
Applications
WB, IB, PCA
Ebihara N et al. Analgesic Effect of Combined Therapy with the Japanese Herbal Medicine “Yokukansan” and Electroacupuncture in Rats with Acute Inflammatory Pain. Medicines (Basel). (2021)
Applications
WB, IB, PCA
Steger M et al. Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale. Nat Commun. (2021)
Applications
WB, IB, PCA
Schroder A et al. Impact of Leptin on Periodontal Ligament Fibroblasts during Mechanical Strain. Int J Mol Sci. (2021)
Applications
WB, IB, PCA
Groeger M et al. Effects of histamine on human periodontal ligament fibroblasts under simulated orthodontic pressure. PLoS One. (2020)
Applications
WB, IB, PCA
Schroder A et al. Role of Oxygen Supply in Macrophages in a Model of Simulated Orthodontic Tooth Movement. Mediators Inflamm. (2020)
Applications
WB, IB, PCA
Schottelndreier D et al. Low Density Lipoprotein Receptor-Related Protein-1 (LRP1) Is Involved in the Uptake of Clostridioides difficile Toxin A and Serves as an Internalizing Receptor. Front Cell Infect Microbiol. (2020)
Applications
WB, IB, PCA
Kirschneck C et al. Role and Regulation of Mechanotransductive HIF-1α Stabilisation in Periodontal Ligament Fibroblasts. Int J Mol Sci. (2020)
Applications
WB, IB, PCA
Jiang C et al. Regulation of Mitochondrial Respiratory Chain Complex Levels, Organization, and Function by Arginyltransferase 1. Front Cell Dev Biol (2020)
Applications
WB, IB, PCA
Timon-Gomez A et al. Distinct roles of mitochondrial HIGD1A and HIGD2A in respiratory complex and supercomplex biogenesis. Cell Rep. (2020)
Applications
WB, IB, PCA
Timon-Gomez A et al. Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis. STAR Protoc. (2020)
Applications
WB, IB, PCA
Sundaramurthy S. et al. FHOD-1 is the only formin in Caenorhabditis elegans that promotes striated muscle growth and Z-line organization in a cell autonomous manner. Cytoskeleton (Hoboken). (2020)
Applications
WB, IB, PCA
Tomaszewska E, Dobrowolski P, Świątkiewicz M, Donaldson J, Puzio I, Muszyński S. Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts? Animals (Basel). (2020)
Applications
IHC, ICC, Histology
Ramirez DH, Aonbangkhen C, Wu HY, et al. Engineering a Proximity-Directed O-GlcNAc Transferase for Selective Protein O-GlcNAcylation in Cells. ACS Chem Biol. (2020)
Applications
WB, IB, PCA
Genth et al. Difference in Mono-O-Glucosylation of Ras Subtype GTPases Between Toxin A and Toxin B From Clostridioides difficile Strain 10463 and Lethal Toxin From Clostridium sordellii Strain 6018. Frontiers in Microbiology (2019)
Applications
WB, IB, PCA
Bastos AEP, Costa PF, Varderidou-Minasian S, Altelaar M, Lima PA. Feeding cycle alters the biophysics and molecular expression of voltage-gated Na+ currents in rat hippocampal CA1 neurones. Eur J Neurosci. (2019)
Applications
WB, IB, PCA
Wang XX, Zhang H, Li Y. Preliminary study on the role of miR‑148a and DNMT1 in the pathogenesis of acute myeloid leukemia. Mol Med Rep. (2019)
Applications
WB, IB, PCA
Henninger C, Pohlmann S, Ziegler V, Ohlig J, Schmitt J, Fritz G. Distinct contribution of Rac1 expression in cardiomyocytes to anthracycline-induced cardiac injury. Biochem Pharmacol. (2019)
Applications
Undefined
Sanderson SM, Mikhael PG, Ramesh V, Dai Z, Locasale JW. Nutrient availability shapes methionine metabolism in p16/MTAP-deleted cells. Sci Adv. (2019)
Applications
WB, IB, PCA
Lin N, Dong XJ, Wang TY, et al. Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841). Mol Med Rep. (2019)
Applications
WB, IB, PCA
Flaxman HA, Miyamoto DK, Woo CM. Small Molecule Interactome Mapping by Photo-Affinity Labeling (SIM-PAL) to Identify Binding Sites of Small Molecules on a Proteome-Wide Scale. Curr Protoc Chem Biol. (2019)
Applications
Undefined
Schröder A, Neubert P, Titze J, et al. Osteoprotective action of low-salt diet requires myeloid cell-derived NFAT5. JCI Insight. (2019)
Applications
WB, IB, PCA
Pearson K. A Search for Multiplanet Systems with TESS Using a Bayesian N-body Retrieval and Machine Learning. The Astronomical Journal (2019)
Applications
Undefined
Song et al. Adeno-associated virus serotype 9 mediated vascular endothelial growth factor gene overexpression in mdx mice. Experimental and Therapeutic Medicine (2018)
Applications
WB, IB, PCA
Atta et al. Thymoquinone Attenuates Cardiomyopathy in Streptozotocin-Treated Diabetic Rats. Oxidative Medicine and Cellular Longevity (2018)
Applications
WB, IB, PCA
Schöttelndreier et al. Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B. Frontiers in Microbiology (2018)
Applications
WB, IB, PCA
Adachi N, Suzuki S, Matsuoka H, et al. Corticotropin-releasing hormone-binding protein is up-regulated by brain-derived neurotrophic factor and is secreted in an activity-dependent manner in rat cerebral cortical neurons. J Neurochem. (2018)
Applications
WB, IB, PCA
Paredes-Amaya CC, Valdés-García G, Juárez-González VR, Rudiño-Piñera E, Bustamante VH. The Hcp-like protein HilE inhibits homodimerization and DNA binding of the virulence-associated transcriptional regulator HilD in Salmonella. J Biol Chem. (2018)
Applications
WB, IB, PCA
Lobo-Jarne T, Nývltová E, Pérez-Pérez R, et al. Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics. Cell Rep. (2018)
Applications
WB, IB, PCA
Rahman MT, Ghosh C, Hossain M, et al. IFN-γ, IL-17A, or zonulin rapidly increase the permeability of the blood-brain and small intestinal epithelial barriers: Relevance for neuro-inflammatory diseases. Biochem Biophys Res Commun. (2018)
Applications
WB, IB, PCA
Castaño et al. Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels. PLOS One (2017)
Applications
WB, IB, PCA
Phan et al. Identification of a substrate domain that determines system specificity in mycobacterial type VII secretion systems. Scientific Reports (2017)
Applications
WB, IB, PCA
Sullivan et al. TARP γ-2 Is Required for Inflammation-Associated AMPA Receptor Plasticity within Lamina II of the Spinal Cord Dorsal Horn. The Journal of Neuroscience (2017)
Applications
WB, IB, PCA
Liang et al. Increased bone morphogenetic protein-6 in follicular fluid and granulosa cells may correlate with fertilization and embryo quality in humans. Experimental and Therapeutic Medicine (2017)
Applications
WB, IB, PCA
Rohrbeck et al. The Rho ADP-ribosylating C3 exoenzyme binds cells via an Arg-Gly-Asp motif. Journal of Biological Chemistry (2017)
Applications
WB, IB, PCA
Liberti et al. A Predictive Model for Selective Targeting of the Warburg Effect through GAPDH Inhibition with a Natural Product. Cell Metabolism (2017)
Applications
WB, IB, PCA
Jeong YK et al. Docosahexaenoic acid inhibits cerulein-induced acute pancreatitis in rats. Nutrients. (2017)
Applications
IHC, ICC, Histology
Simhadri VL, Hamasaki-Katagiri N, Lin BC, et al. Single synonymous mutation in factor IX alters protein properties and underlies haemophilia B. J Med Genet. (2017)
Applications
E, EIA; WB, IB, PCA
González-Stegmaier R, Villarroel-Espíndola F, Manríquez R, et al. New immunomodulatory role of neuropeptide Y (NPY) in Salmo salar leucocytes. Dev Comp Immunol. (2017)
Applications
WB, IB, PCA
Liu Y, Wang C, Shan X, et al. S100P is associated with proliferation and migration in nasopharyngeal carcinoma. Oncol Lett. (2017)
Applications
WB, IB, PCA
Jong WS et al. Application of an E. coli signal sequence as a versatile inclusion body tag. Microbial Cell Factories (2017)
Applications
WB, IB, PCA
Wei et al. Interleukin-17 potently increases non-small cell lung cancer growth. Molecular Medicine Reports (2016)
Applications
WB, IB, PCA
Zhao et al. Paris Saponins enhance radiosensitivity in a gefitinib-resistant lung adenocarcinoma cell line by inducing apoptosis and G2/M cell cycle phase arrest. Molecular Medicine Reports (2016)
Applications
WB, IB, PCA
Yao et al. Elevated expression of chemokine C-C ligand 2 in stroma is associated with recurrent basal-like breast cancers. Modern Pathology (2016)
Applications
E, EIA; IHC, ICC, Histology; WB, IB, PCA
Schelle et al. Role of p38alpha/beta MAP Kinase in Cell Susceptibility to Clostridium sordellii Lethal Toxin and Clostridium difficile Toxin B. Toxins (2016)
Applications
WB, IB, PCA
Mendez et al. Endoplasmic reticulum stress-independent activation of unfolded protein response kinases by a small molecule ATP-mimic. Elife (2015)
Applications
WB, IB, PCA
Fang et al. TGF-β Negatively Regulates CXCL1 Chemokine Expression in Mammary Fibroblasts through Enhancement of Smad2/3 and Suppression of HGF/c-Met Signaling Mechanisms. PLOS One (2015)
Applications
WB, IB, PCA
Stephen et al. Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling. The Journal of Neuroscience (2015)
Applications
WB, IB, PCA
Koretsi V, Kirschneck C, Proff P, Römer P. Expression of glutathione peroxidase 1 in the spheno-occipital synchondrosis and its role in ROS-induced apoptosis. Eur J Orthod. (2015)
Applications
IHC, ICC, Histology
Galvão BP, Weber BW, Rafudeen MS, Ferreira EO, Patrick S, Abratt VR. Identification of a collagen type I adhesin of Bacteroides fragilis. PLoS One. (2014)
Applications
WB, IB, PCA
VanDuyn N, Settivari R, LeVora J, Zhou S, Unrine J, Nass R. The metal transporter SMF-3/DMT-1 mediates aluminum-induced dopamine neuron degeneration. J Neurochem. (2013)
Applications
WB, IB, PCA
Junk DJ, Cipriano R, Stampfer M, Jackson MW. Constitutive CCND1/CDK2 activity substitutes for p53 loss, or MYC or oncogenic RAS expression in the transformation of human mammary epithelial cells. PLoS One. (2013)
Applications
WB, IB, PCA
Settivari R, VanDuyn N, LeVora J, Nass R. The Nrf2/SKN-1-dependent glutathione S-transferase π homologue GST-1 inhibits dopamine neuron degeneration in a Caenorhabditis elegans model of manganism. Neurotoxicology (2013)
Applications
WB, IB, PCA
Springhorn C et al. Exploring leukocyte O-GlcNAcylation as a novel diagnostic tool for the earlier detection of type 2 diabetes mellitus. J Clin Endocrinol Metab. (2012)
Applications
WB, IB, PCA
Horn P et al. Circulating microparticles carry a functional endothelial nitric oxide synthase that is decreased in patients with endothelial dysfunction. J Am Heart Assoc. (2012)
Applications
WB, IB, PCA
Pop V et al. Aβ aggregation profiles and shifts in APP processing favor amyloidogenesis in canines. Neurobiology of Aging (2012)
Applications
WB, IB, PCA
Zong M, Satoh A, Yu MK, et al. TRAPPC9 mediates the interaction between p150 and COPII vesicles at the target membrane. PLoS One. (2012)
Applications
WB, IB, PCA
Pullen NA, Anand M, Cooper PS, Fillmore HL. Matrix metalloproteinase-1 expression enhances tumorigenicity as well as tumor-related angiogenesis and is inversely associated with TIMP-4 expression in a model of glioblastoma. J Neurooncol. (2012)
Applications
Undefined
Blockx I, Verhoye M, Van Audekerke J, et al. Identification and characterization of Huntington related pathology: an in vivo DKI imaging study. Neuroimage (2012)
Applications
WB, IB, PCA
Daleke MH, van der Woude AD, Parret AH, et al. Specific chaperones for the type VII protein secretion pathway. J Biol Chem. (2012)
Applications
WB, IB, PCA
Wu CY. Annexin A6 Modulates Chick Cranial Neural Crest Cell Emigration. PLoS One. (2012)
Applications
WB, IB, PCA
Daleke MH, Cascioferro A, de Punder K, et al. Conserved Pro-Glu (PE) and Pro-Pro-Glu (PPE) protein domains target LipY lipases of pathogenic mycobacteria to the cell surface via the ESX-5 pathway. J Biol Chem. (2011)
Applications
WB, IB, PCA
Romberger SP et al. Identification and characterization of PshBII, a second FA/FB-containing polypeptide in the photosynthetic reaction center of Heliobacterium modesticaldum. Photosynth Res. (2010)
Applications
WB, IB, PCA
Niedowicz DM et al. APP(DeltaNL695) expression in murine tissue downregulates CNBP expression. Neuroscience Letters (2010)
Applications
WB, IB, PCA
Pop V et al. Synergistic effects of long-term antioxidant diet and behavioral enrichment on beta-amyloid load and non-amyloidogenic processing in aged canines. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience (2010)
Applications
WB, IB, PCA
Pullen NA, Fillmore HL. Induction of matrix metalloproteinase-1 and glioma cell motility by nitric oxide. J Neurooncol. (2010)
Applications
Undefined
Levitin A, Yanofsky C. Positions of Trp codons in the leader peptide-coding region of the at operon influence anti-trap synthesis and trp operon expression in Bacillus licheniformis. J Bacteriol. (2010)
Applications
WB, IB, PCA
Vanduyn N, Settivari R, Wong G, Nass R. SKN-1/Nrf2 inhibits dopamine neuron degeneration in a Caenorhabditis elegans model of methylmercury toxicity. Toxicol Sci. (2010)
Applications
WB, IB, PCA
Xue H, Liu S, Ji T, et al. Expression of NKCC2 in the rat gastrointestinal tract. Neurogastroenterol Motil. (2009)
Applications
WB, IB, PCA; IHC, ICC, Histology
King JC. et al. Thermal-hydraulic and neutronic analyses of the submersion-subcritical, safe space (S4) reactor. Nuclear engineering and design (2009)
Applications
Undefined
Palmer GE. Chapter Twenty‐One Autophagy in Candida albicans. Methods in Enzymology (2008)
Applications
Undefined
Liu X. STAT3 activation inhibits human bronchial epithelial cell apoptosis in response to cigarette smoke exposure. Biochem Biophys Res Commun. (2007)
Applications
Undefined
Liu X, Das AM, Seideman J, et al. The CC chemokine ligand 2 (CCL2) mediates fibroblast survival through IL-6. Am J Respir Cell Mol Biol. (2007)
Applications
E, EIA; WB, IB, PCA
Jamerson MH, Johnson MD, Korsmeyer SJ, Furth PA, Dickson RB. Bax regulates c-Myc-induced mammary tumour apoptosis but not proliferation in MMTV-c-myc transgenic mice. Br J Cancer. (2004)
Applications
WB, IB, PCA
Bednarska-Boleka B. et al. Vibrational study of the structural phase transition in bis(pyrrolidinium)-chloride-hexachloroantimonate(V) by infrared spectroscopy. Journal of Molecular Structure (2002)
Applications
Undefined
Renard P, Ernest I, Houbion A, et al. Development of a sensitive multi-well colorimetric assay for active NFkappaB. Nucleic Acids Res. (2001)
Applications
Other

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.