Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

Rabbit IgG (H&L) Secondary Antibody Peroxidase Conjugated

Goat Polyclonal

611-1302
2 mg
ELISA; ELISA; WB
Goat
Have a Question? Ask a Specialist
$130.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Anti-Rabbit IgG Antibody peroxidase generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to peroxidase.

Synonyms

Goat anti-Rabbit IgG Secondary Antibody Peroxidase Conjugated, Goat anti-Rabbit IgG Secondary Antibody HRP Conjugated, GAR-HRP, Anti-rabbit secondary antibody, anti-rabbit HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-rabbit horseradish peroxidase conjugated secondary antibody

Immunogen

Rabbit IgG whole molecule

Application Note

This anti-rabbit HRP secondary antibody is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Purity/Specificity

RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Storage Condition

Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302
ELISA
ELISA; WB
Rabbit
Goat
Peroxidase (Horseradish)
Polyclonal
IgG
Lyophilized
Ambient
Expiration date is one (1) year from date of opening.
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Restore with deionized water (or equivalent)
1.0 mL

Invalid lot number

If you need help finding your CofA, contact us

  • Constitutive CCND1/CDK2 activity substitutes for p53 loss, or MYC or oncogenic RAS expression in the transformation of human mammary epithelial cells.;203;Junk DJ, Cipriano R, Stampfer M, Jackson MW.;PubMed
  • Impact of salt and the osmoprotective transcription factor NFAT‐5 on macrophages during mechanical strain.;2021;Schroder A et al.;PubMed
  • Dietary Salt Accelerates Orthodontic Tooth Movement by Increased Osteoclast Activity.;2021;Schroder A et al.;PubMed
  • Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model.;2021;Puzio I et al.;PubMed
  • Effects of histamine on human periodontal ligament fibroblasts under simulated orthodontic pressure.;2020;Groeger M et al.;PubMed
  • Role of Oxygen Supply in Macrophages in a Model of Simulated Orthodontic Tooth Movement.;2020;Schroder A et al.;PubMed
  • Low Density Lipoprotein Receptor-Related Protein-1 (LRP1) Is Involved in the Uptake of Clostridioides difficile Toxin A and Serves as an Internalizing Receptor.;2020;Schottelndreier D et al.;PubMed
  • Role and Regulation of Mechanotransductive HIF-1α Stabilisation in Periodontal Ligament Fibroblasts.;2020;Kirschneck C et al.;PubMed
  • Regulation of Mitochondrial Respiratory Chain Complex Levels, Organization, and Function by Arginyltransferase 1.;2020;Jiang C et al.;PubMed
  • Distinct roles of mitochondrial HIGD1A and HIGD2A in respiratory complex and supercomplex biogenesis.;2020;Timon-Gomez A et al.;PubMed
  • Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis.;2020;Timon-Gomez A et al.;PubMed
  • FHOD-1 is the only formin in Caenorhabditis elegans that promotes striated muscle growth and Z-line organization in a cell autonomous manner.;2020;Sundaramurthy S. et al.;PubMed
  • Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts?;2020;Tomaszewska E, Dobrowolski P, Świątkiewicz M, Donaldson J, Puzio I, Muszyński S.;PubMed
  • Engineering a Proximity-Directed O-GlcNAc Transferase for Selective Protein O-GlcNAcylation in Cells.;2020;Ramirez DH, Aonbangkhen C, Wu HY, et al.;PubMed
  • Difference in Mono-O-Glucosylation of Ras Subtype GTPases Between Toxin A and Toxin B From Clostridioides difficile Strain 10463 and Lethal Toxin From Clostridium sordellii Strain 6018.;2019;Genth et al.;PubMed
  • Feeding cycle alters the biophysics and molecular expression of voltage-gated Na+ currents in rat hippocampal CA1 neurones.;2019;Bastos AEP, Costa PF, Varderidou-Minasian S, Altelaar M, Lima PA.;PubMed
  • Preliminary study on the role of miR‑148a and DNMT1 in the pathogenesis of acute myeloid leukemia.;2019;Wang XX, Zhang H, Li Y.;PubMed
  • Distinct contribution of Rac1 expression in cardiomyocytes to anthracycline-induced cardiac injury.;2019;Henninger C, Pohlmann S, Ziegler V, Ohlig J, Schmitt J, Fritz G.;PubMed
  • Nutrient availability shapes methionine metabolism in p16/MTAP-deleted cells.;2019;Sanderson SM, Mikhael PG, Ramesh V, Dai Z, Locasale JW.;PubMed
  • Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841).;2019;Lin N, Dong XJ, Wang TY, et al.;PubMed
  • Small Molecule Interactome Mapping by Photo-Affinity Labeling (SIM-PAL) to Identify Binding Sites of Small Molecules on a Proteome-Wide Scale.;2019;Flaxman HA, Miyamoto DK, Woo CM.;PubMed
  • Osteoprotective action of low-salt diet requires myeloid cell-derived NFAT5.;2019;Schröder A, Neubert P, Titze J, et al.;PubMed
  • A Search for Multiplanet Systems with TESS Using a Bayesian N-body Retrieval and Machine Learning.;2019;Pearson K.;PubMed
  • Adeno-associated virus serotype 9 mediated vascular endothelial growth factor gene overexpression in mdx mice.;2018;Song et al.;PubMed
  • Thymoquinone Attenuates Cardiomyopathy in Streptozotocin-Treated Diabetic Rats.;2018;Atta et al.;PubMed
  • Expression and (Lacking) Internalization of the Cell Surface Receptors of Clostridioides difficile Toxin B.;2018;Schöttelndreier et al.;PubMed
  • Corticotropin-releasing hormone-binding protein is up-regulated by brain-derived neurotrophic factor and is secreted in an activity-dependent manner in rat cerebral cortical neurons.;2018;Adachi N, Suzuki S, Matsuoka H, et al.;PubMed
  • The Hcp-like protein HilE inhibits homodimerization and DNA binding of the virulence-associated transcriptional regulator HilD in Salmonella.;2018;Paredes-Amaya CC, Valdés-García G, Juárez-González VR, Rudiño-Piñera E, Bustamante VH.;PubMed
  • Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics.;2018;Lobo-Jarne T, Nývltová E, Pérez-Pérez R, et al.;PubMed
  • IFN-γ, IL-17A, or zonulin rapidly increase the permeability of the blood-brain and small intestinal epithelial barriers: Relevance for neuro-inflammatory diseases.;2018;Rahman MT, Ghosh C, Hossain M, et al.;PubMed
  • Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels.;2017;Castaño et al.;PubMed
  • Identification of a substrate domain that determines system specificity in mycobacterial type VII secretion systems.;2017;Phan et al.;PubMed
  • TARP γ-2 Is Required for Inflammation-Associated AMPA Receptor Plasticity within Lamina II of the Spinal Cord Dorsal Horn.;2017;Sullivan et al.;PubMed
  • Increased bone morphogenetic protein-6 in follicular fluid and granulosa cells may correlate with fertilization and embryo quality in humans.;2017;Liang et al.;PubMed
  • The Rho ADP-ribosylating C3 exoenzyme binds cells via an Arg-Gly-Asp motif.;2017;Rohrbeck et al.;PubMed
  • A Predictive Model for Selective Targeting of the Warburg Effect through GAPDH Inhibition with a Natural Product.;2017;Liberti et al.;PubMed
  • Docosahexaenoic acid inhibits cerulein-induced acute pancreatitis in rats.;2017;Jeong YK et al.;PubMed
  • Single synonymous mutation in factor IX alters protein properties and underlies haemophilia B.;2017;Simhadri VL, Hamasaki-Katagiri N, Lin BC, et al.;PubMed
  • New immunomodulatory role of neuropeptide Y (NPY) in Salmo salar leucocytes.;2017;González-Stegmaier R, Villarroel-Espíndola F, Manríquez R, et al.;PubMed
  • S100P is associated with proliferation and migration in nasopharyngeal carcinoma.;2017;Liu Y, Wang C, Shan X, et al.;PubMed
  • Application of an E. coli signal sequence as a versatile inclusion body tag.;2017;Jong WS et al.;PubMed
  • Interleukin-17 potently increases non-small cell lung cancer growth.;2016;Wei et al.;PubMed
  • Paris Saponins enhance radiosensitivity in a gefitinib-resistant lung adenocarcinoma cell line by inducing apoptosis and G2/M cell cycle phase arrest.;2016;Zhao et al.;PubMed
  • Elevated expression of chemokine C-C ligand 2 in stroma is associated with recurrent basal-like breast cancers.;2016;Yao et al.;PubMed
  • Role of p38alpha/beta MAP Kinase in Cell Susceptibility to Clostridium sordellii Lethal Toxin and Clostridium difficile Toxin B.;2016;Schelle et al.;PubMed
  • Endoplasmic reticulum stress-independent activation of unfolded protein response kinases by a small molecule ATP-mimic.;2015;Mendez et al.;PubMed
  • TGF-β Negatively Regulates CXCL1 Chemokine Expression in Mammary Fibroblasts through Enhancement of Smad2/3 and Suppression of HGF/c-Met Signaling Mechanisms.;2015;Fang et al.;PubMed
  • Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling.;2015;Stephen et al.;PubMed
  • Expression of glutathione peroxidase 1 in the spheno-occipital synchondrosis and its role in ROS-induced apoptosis.;2015;Koretsi V, Kirschneck C, Proff P, Römer P.;PubMed
  • Identification of a collagen type I adhesin of Bacteroides fragilis.;2014;Galvão BP, Weber BW, Rafudeen MS, Ferreira EO, Patrick S, Abratt VR.;PubMed
  • The metal transporter SMF-3/DMT-1 mediates aluminum-induced dopamine neuron degeneration.;2013;VanDuyn N, Settivari R, LeVora J, Zhou S, Unrine J, Nass R.;PubMed
  • The Nrf2/SKN-1-dependent glutathione S-transferase π homologue GST-1 inhibits dopamine neuron degeneration in a Caenorhabditis elegans model of manganism.;2013;Settivari R, VanDuyn N, LeVora J, Nass R.;PubMed
  • Exploring leukocyte O-GlcNAcylation as a novel diagnostic tool for the earlier detection of type 2 diabetes mellitus.;2012;Springhorn C et al.;PubMed
  • Circulating microparticles carry a functional endothelial nitric oxide synthase that is decreased in patients with endothelial dysfunction.;2012;Horn P et al.;PubMed
  • Aβ aggregation profiles and shifts in APP processing favor amyloidogenesis in canines.;2012;Pop V et al.;PubMed
  • TRAPPC9 mediates the interaction between p150 and COPII vesicles at the target membrane.;2012;Zong M, Satoh A, Yu MK, et al.;PubMed
  • Matrix metalloproteinase-1 expression enhances tumorigenicity as well as tumor-related angiogenesis and is inversely associated with TIMP-4 expression in a model of glioblastoma.;2012;Pullen NA, Anand M, Cooper PS, Fillmore HL.;PubMed
  • Identification and characterization of Huntington related pathology: an in vivo DKI imaging study.;2012;Blockx I, Verhoye M, Van Audekerke J, et al.;PubMed
  • Specific chaperones for the type VII protein secretion pathway.;2012;Daleke MH, van der Woude AD, Parret AH, et al.;PubMed
  • Annexin A6 Modulates Chick Cranial Neural Crest Cell Emigration.;2012;Wu CY.;PubMed
  • Conserved Pro-Glu (PE) and Pro-Pro-Glu (PPE) protein domains target LipY lipases of pathogenic mycobacteria to the cell surface via the ESX-5 pathway.;2011;Daleke MH, Cascioferro A, de Punder K, et al.;PubMed
  • Identification and characterization of PshBII, a second FA/FB-containing polypeptide in the photosynthetic reaction center of Heliobacterium modesticaldum.;2010;Romberger SP et al.;PubMed
  • APP(DeltaNL695) expression in murine tissue downregulates CNBP expression.;2010;Niedowicz DM et al.;PubMed
  • Synergistic effects of long-term antioxidant diet and behavioral enrichment on beta-amyloid load and non-amyloidogenic processing in aged canines.;2010;Pop V et al.;PubMed
  • Induction of matrix metalloproteinase-1 and glioma cell motility by nitric oxide.;2010;Pullen NA, Fillmore HL.;PubMed
  • Positions of Trp codons in the leader peptide-coding region of the at operon influence anti-trap synthesis and trp operon expression in Bacillus licheniformis.;2010;Levitin A, Yanofsky C.;PubMed
  • SKN-1/Nrf2 inhibits dopamine neuron degeneration in a Caenorhabditis elegans model of methylmercury toxicity.;2010;Vanduyn N, Settivari R, Wong G, Nass R.;PubMed
  • Expression of NKCC2 in the rat gastrointestinal tract.;2009;Xue H, Liu S, Ji T, et al.;PubMed
  • Thermal-hydraulic and neutronic analyses of the submersion-subcritical, safe space (S4) reactor.;2009;King JC. et al.;PubMed
  • Chapter Twenty‐One Autophagy in Candida albicans.;2008;Palmer GE.;PubMed
  • STAT3 activation inhibits human bronchial epithelial cell apoptosis in response to cigarette smoke exposure.;2007;Liu X.;PubMed
  • The CC chemokine ligand 2 (CCL2) mediates fibroblast survival through IL-6.;2007;Liu X, Das AM, Seideman J, et al.;PubMed
  • Bax regulates c-Myc-induced mammary tumour apoptosis but not proliferation in MMTV-c-myc transgenic mice.;2004;Jamerson MH, Johnson MD, Korsmeyer SJ, Furth PA, Dickson RB.;PubMed
  • Vibrational study of the structural phase transition in bis(pyrrolidinium)-chloride-hexachloroantimonate(V) by infrared spectroscopy.;2002;Bednarska-Boleka B. et al.;PubMed
  • Development of a sensitive multi-well colorimetric assay for active NFkappaB.;2001;Renard P, Ernest I, Houbion A, et al.;PubMed