Rabbit IgG (H&L) Antibody DyLight™ 549 Conjugated
Dylight conjugated antibody - Western Blot
DyLight™ 549 Fluorescence Spectra.
Properties of DyLight™ Fluorescent Dyes.
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Datasheet

Rabbit IgG (H&L) Antibody DyLight™ 549 Conjugated

Goat Polyclonal

611-142-002 611-142-002-0.5
100 µg 5 x 100 µg
Dot Blot, IF, WB
Rabbit
Goat
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$95.00 /Per Item
$164.00 /Per Item
100 µg $95.00
5 x 100 µg $164.00
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Anti-Rabbit IgG (H&L) DyLight 549 Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Note

Anti-Rabbit IgG (H&L) DyLight 549 Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. This antibody will react with heavy chains of Rabbit IgG and with light chains of most Rabbit immunoglobulins.

Anti-RABBIT IgG (H&L) (GOAT) Antibody DyLight™ 549 Conjugated - 611-142-002
Goat anti-Rabbit IgG Antibody DyLight™549 Conjugation, Goat anti-Rabbit IgG DyLight™ 549 Conjugated Antibody
Dot Blot
IF, WB
Rabbit
Rabbit
Goat
DyLight™ 549
Polyclonal
IgG
Rabbit IgG whole molecule
7 moles DL549 per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Jayanthi B et al. A platform for post-translational spatiotemporal control of cellular proteins. Synth Biol (Oxf). (2021)
Applications
IF, Confocal Microscopy
Sanchez MM et al. Generation of Self-assembled Vascularized Human Skin Equivalents. J Vis Exp. (2021)
Applications
IF, Confocal Microscopy
Lu X et al. 17β‑hydroxysteroid dehydrogenase 4 induces liver cancer proliferation‑associated genes via STAT3 activation. Oncol Rep. (2019)
Applications
WB, IB, PCA
Legaspi SM et al. Aggregation behavior of nanoparticle-peptide systems affects autophagy. Bioconjug Chem. (2019)
Applications
IF, Confocal Microscopy
Saumweber et al. Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila. Nature Communications (2018)
Applications
MultiColor FlpOut
Popp L et al. Autophagic response to cellular exposure to titanium dioxide nanoparticles. Acta Biomater. (2018)
Applications
IF, Confocal Microscopy
Popp L et al. TFEB-mediated activation of the lysosome-autophagy system affects the transduction efficiency of adeno-associated virus 2. Virology. (2017)
Applications
IF, Confocal Microscopy
De Luigi A et al. Doxycycline hinders phenylalanine fibril assemblies revealing a potential novel therapeutic approach in phenylketonuria. Scientific Reports (2015)
Applications
IHC, ICC, Histology

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.