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Background

Anti-Rabbit IgG Antibody DyLight™800 generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to DyLight™800.

Product Details

Goat Anti-Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated - 611-145-002
Goat anti-Rabbit IgG Antibody DyLight™800 Conjugation, Goat anti-Rabbit IgG DyLight™ 800 Conjugated Antibody
Goat
IgG (H&L)
DyLight™ 800
Polyclonal
IgG

Target Details

Rabbit
Rabbit IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. This antibody will react with heavy chains of Rabbit IgG and with light chains of most Rabbit immunoglobulins.

Application Details

Dot Blot, ELISA, WB
IHC, IP  - View References
Anti-Rabbit IgG Antibody DyLight™800 has been tested by ELISA, dot blot, and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). Destabilization of fear memory by Rac1-driven engram-microglia communication in hippocampus. Brain Behav Immun.
Applications
WB, IB, PCA
(). Atrial proteomic profiling reveals a switch towards profibrotic gene expression program in CREM-IbΔC-X mice with persistent atrial fibrillation. J Mol Cell Cardiol.
Applications
WB, IB, PCA
(). The impact of Nrf2 knockout on the neuroprotective effects of dexmedetomidine in a mice model of cognitive impairment. Behav Brain Res.
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WB, IB, PCA
(). Downregulated expression of lactate dehydrogenase in adult oligodendrocytes and its implication for the transfer of glycolysis products to axons. Glia.
Applications
WB, IB, PCA
(). A multimorphic mutation in IRF4 causes human autosomal dominant combined immunodeficiency. Sci Immunol.
Applications
Supershift, Gel Shift, EMSA
(). Bioinformatics and network pharmacology identify promotional effects and potential mechanisms of ethanol on esophageal squamous cell carcinoma and experimental validation. Toxicol Appl Pharmacol.
Applications
WB, IB, PCA
(). A whole transcriptome profiling analysis for antidepressant mechanism of Xiaoyaosan mediated synapse loss via BDNF/trkB/PI3K signal axis in CUMS rats. BMC Complement Med Ther.
Applications
WB, IB, PCA
(). Testosterone reduces hippocampal synaptic damage in an androgen receptor-independent manner. J Endocrinol.
Applications
WB, IB, PCA
(). SARS-CoV-2 N protein induced acute kidney injury in diabetic db/db mice is associated with a Mincle-dependent M1 macrophage activation. Front Immunol.
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WB, IB, PCA
(). Targeting of HBP1/TIMP3 axis as a novel strategy against breast cancer. Pharmacol Res.
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WB, IB, PCA
(). The transcription factor HBP1 promotes ferroptosis in tumor cells by regulating the UHRF1-CDO1 axis. PLoS Biol.
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WB, IB, PCA
(). Paclitaxel induces cognitive impairment via necroptosis, decreased synaptic plasticity and M1 polarisation of microglia. Pharmaceutical Biology
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WB, IB, PCA
(). Casein kinase 2 attenuates brain injury induced by intracerebral hemorrhage via regulation of NR2B phosphorylation. Frontiers in Cellular Neuroscience
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WB, IB, PCA
(). RNA-based therapies in animal models of Leber congenital amaurosis causing blindness. Precision Clinical Medicine
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WB, IB, PCA
(). Fragile X Mental Retardation Protein Mediates the Effects of Androgen on Hippocampal PSD95 Expression and Dendritic Spines Density/Morphology and Autism-Like Behaviors Through miR-125a. Frontiers in Cellular Neuroscience
Applications
WB, IB, PCA
(). L-plastin enhances NLRP3 inflammasome assembly and bleomycin-induced lung fibrosis. Cell Reports
Applications
WB, IB, PCA
(). Nrf2 improves hippocampal synaptic plasticity, learning and memory through the circ-Vps41/miR-26a-5p/CaMKIV regulatory network. Exp Neurol.
Applications
WB, IB, PCA
(). Molecular conflicts disrupting centromere maintenance contribute to Xenopus hybrid inviability. Curr Biol.
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WB, IB, PCA
(). HBP1 inhibits the development of type 2 diabetes mellitus through transcriptional activation of the IGFBP1 gene. Aging (Albany NY).
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WB, IB, PCA
(). CaMKII is a modulator in neurodegenerative diseases and mediates the effect of androgen on synaptic protein PSD95. Front Genet.
Applications
WB, IB, PCA
(). Knockdown of METTL16 disrupts learning and memory by reducing the stability of MAT2A mRNA. Cell Death Disc.
Applications
WB, IB, PCA; Dot Blot Assay
(). Collateral deletion of the mitochondrial AAA+ ATPase ATAD1 sensitizes cancer cells to proteasome dysfunction. Elife.
Applications
WB, IB, PCA
(). Pharmacological suppression of Nedd4-2 rescues the reduction of Kv11.1 channels in pathological cardiac hypertrophy. Front Pharmacol.
Applications
WB, IB, PCA
(). IL-6 promotes nuclear translocation of HIF-1α to aggravate chemoresistance of ovarian cancer cells. Eur J Pharmacol.
Applications
WB, IB, PCA
(). Mitochondrial morphology, bioenergetics and proteomic responses in fatty acid oxidation disorders. Redox Biol.
Applications
WB, IB, PCA
(). HBP1-mediated transcriptional repression of AFP inhibits hepatoma progression. J Exp Clin Cancer Res.
Applications
WB, IB, PCA
(). GP73-mediated secretion of AFP and GP73 promotes proliferation and metastasis of hepatocellular carcinoma cells. Oncogenesis.
Applications
WB, IB, PCA
(). Icaritin promotes apoptosis and inhibits proliferation by down-regulating AFP gene expression in hepatocellular carcinoma. BMC Cancer.
Applications
WB, IB, PCA
(). Autoregulatory control of microtubule binding in doublecortin-like kinase 1. Elife.
Applications
WB, IB, PCA
(). Centriole and Golgi microtubule nucleation are dispensable for the migration of human neutrophil-like cells. Mol Biol Cell.
Applications
WB, IB, PCA
(). Mechanistic understanding of the combined immunodeficiency in complete human CARD11 deficiency. J Allergy Clin Immunol.
Applications
WB, IB, PCA
(). Distinct immune microenvironment profiles of therapeutic responders emerge in combined TGFβ/PD-L1 blockade-treated squamous cell carcinoma. Commun Biol.
Applications
WB, IB, PCA
(). MALT1-Dependent Cleavage of HOIL1 Modulates Canonical NF-κB Signaling and Inflammatory Responsiveness. Front Immunol.
Applications
WB, IB, PCA
(). TGF‐β‐induced IGFBP‐3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion. Mol Oncol.
Applications
WB, IB, PCA
(). Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin. Nat Commun.
Applications
WB, IB, PCA
(). The role of Mediator and Little Elongation Complex in transcription termination. Nat Commun.
Applications
WB, IB, PCA
(). Acetylation of alpha-fetoprotein promotes hepatocellular carcinoma progression. Cancer Lett.
Applications
WB, IB, PCA
(). GCAP neuronal calcium sensor proteins mediate photoreceptor cell death in the rd3 mouse model of LCA12 congenital blindness by involving endoplasmic reticulum stress. Cell Death Dis.
Applications
WB, IB, PCA
(). Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria. Elife
Applications
WB, IB, PCA
(). RNF115 deletion inhibits autophagosome maturation and growth of gastric cancer Cell Death Dis.
Applications
WB, IB, PCA
(). Structural myelin defects are associated with low axonal ATP levels but rapid recovery from energy deprivation in a mouse model of spastic paraplegia. PLoS Biol.
Applications
WB, IB, PCA
(). ERK-dependent suicide gene therapy for selective targeting of RTK/RAS-driven cancers. Mol Ther.
Applications
WB, IB, PCA
(). MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1. Oncogene
Applications
WB, IB, PCA
(). Deletion of TMEM268 inhibits growth of gastric cancer cells by downregulating the ITGB4 signaling pathway. Cell Death Differ.
Applications
WB, IB, PCA
(). An allosteric MALT1 inhibitor is a molecular corrector rescuing function in an immunodeficient patient. Nat Chem Biol.
Applications
WB, IB, PCA
(). Isobavachalcone exerts anti‑proliferative and pro‑apoptotic effects on human liver cancer cells by targeting the ERKs/RSK2 signaling pathway. Oncol Rep.
Applications
WB, IB, PCA
(). RSRC1 suppresses gastric cancer cell proliferation and migration by regulating PTEN expression. Mol Med Rep.
Applications
WB, IB, PCA
(). KCC2 regulates neuronal excitability and hippocampal activity via interaction with Task-3 channels. Cell Rep.
Applications
WB, IB, PCA
(). Arkadia/RNF111 is a SUMO-targeted ubiquitin ligase with preference for substrates marked with SUMO1-capped SUMO2/3 chain. Nat Commun.
Applications
WB, IB, PCA
(). Kif2a scales meiotic spindle size in Hymenochirus boettgeri. Curr Biol.
Applications
WB, IB, PCA
(). Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization. J Vis Exp.
Applications
WB, IB, PCA
(). Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy. Cell Death & Disease
Applications
IHC, ICC, Histology; WB, IB, PCA
(). Amelioratory Effects of Testosterone Propionate on Age-related Renal Fibrosis via Suppression of TGF-β1/Smad Signaling and Activation of Nrf2-ARE Signaling. Scientific Reports
Applications
WB, IB, PCA
(). TNF overproduction impairs epithelial staphylococcal response in hyper IgE syndrome. Journal of Clinical Investigation
Applications
WB, IB, PCA
(). PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation. Int J Oncol.
Applications
WB, IB, PCA
(). A positive feedback loop between Pim-1 kinase and HBP1 transcription factor contributes to hydrogen peroxide-induced premature senescence and apoptosis. Journal of Biological Chemistry
Applications
WB, IB, PCA
(). Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality. Cell Death & Disease
Applications
WB, IB, PCA
(). Efficacy of Postnatal In Vivo Nonsense Suppression Therapy in a Pax6 Mouse Model of Aniridia. Molecular Therapy - Nucleic Acids
Applications
WB, IB, PCA
(). Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress. Cell Death & Disease
Applications
WB, IB, PCA
(). GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl--sensitive WNK1 kinase. Nature Communications
Applications
WB, IB, PCA
(). A hot spot on interferon α/β receptor subunit 1 (IFNAR1) underpins its interaction with interferon-β and dictates signaling. J Biol Chem.
Applications
WB, IB, PCA
(). EMC6/TMEM93 suppresses glioblastoma proliferation by modulating autophagy. Cell Death & Disease
Applications
WB, IB, PCA
(). HBP1-mediated Regulation of p21 Protein through the Mdm2/p53 and TCF4/EZH2 Pathways and Its Impact on Cell Senescence and Tumorigenesis. Journal of Biological Chemistry
Applications
WB, IB, PCA
(). MARCH2 regulates autophagy by promoting CFTR ubiquitination and degradation and PIK3CA-AKT-MTOR signaling. Autophagy.
Applications
WB, IB, PCA
(). PIASxα ligase enhances SUMO1 modification of PTEN protein as a SUMO E3 ligase. J Biol Chem.
Applications
WB, IB, PCA
(). PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1. Autophagy.
Applications
IP, Co-IP
(). A novel ER-localized transmembrane protein, EMC6, interacts with RAB5A and regulates cell autophagy. Autophagy.
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.