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Datasheet

Rabbit IgG (H&L) Antibody Peroxidase Conjugated

Chicken Polyclonal

4 References
611-903-002
1 mg
Lyophilized
WB, ELISA, IHC
Rabbit
Chicken
$210.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Anti-Rabbit IgG (H&L) peroxidase Antibody generated in chicken detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Product Details

Chicken Anti-Rabbit IgG (H&L) Antibody Peroxidase Conjugated - 611-903-002
chicken anti-Rabbit IgG Antibody peroxidase Conjugation, chicken anti-Rabbit IgG HRP Conjugated Antibody
Chicken
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG

Target Details

Rabbit
Native Protein
Anti-Rabbit IgG whole molecule was produced by repeated immunization with Rabbit IgG whole molecule in chicken.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken Serum, Rabbit IgG and Rabbit Serum.

Application Details

ELISA
IHC, WB  - View References
Anti-Rabbit IgG Peroxidase Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). Beneficial effects of atorvastatin on sex‐specific cognitive impairment induced by a cerebral microhaemorrhage in mice. Br J Pharmacol.
Applications
WB, IB, PCA
(). α- and β-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage. Experimental and Therapeutic Medicine
Applications
WB, IB, PCA
(). Experimental infection of gnotobiotic piglets with Escherichia coli strains positive for EAST1 and AIDA. Vet Immunol Immunopathol.
Applications
IHC, ICC, Histology
(). The effect of intramuscular administration of colistin on the development and course of experimentally induced oedema disease in weaned piglets. Vet Microbiol.
Applications
IHC, ICC, Histology

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.