DyLight™ Fluorescent Dye Conjugates

November 15, 2021

 

DyLight™ conjugated antibodies are high-performance fluorescent conjugates designed for use in various primary and secondary antibody assays, including fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening, multiplex assays, and other array platforms. These conjugates are highly functional with bright emission spectra that align with the output wavelengths of common fluorescence instrumentation. The introduction of DyLight™ conjugates has revolutionized the field of fluorescence-based assays, offering researchers tools with high fluorescence intensity, photostability, and compatibility with standard laboratory equipment, ensuring accurate and reliable results across diverse applications.

DyLight™ Fluorochromes

Emission Fluorochrome Abs (nm) Em (nm) MW (daltons) Spectrally similar
Blue DyLight™ 405 420 400 793 Alexa Fluor 350
Green DyLight™ 488 493 518 1,011 Alexa Fluor 488,  Fluorescein
Yellow DyLight™ 549 550 568 982 ATTO 532, Rhodamine, R-Phycoerythrin, Cy3™
Red DyLight™ 649 649 674 1,008 ATTO 655, Allophycocyanin, Cy5™
Near-infrared DyLight™ 680 682 715 950 IRDye®700, Alexa Fluor 680, Cy5.5™
Infrared DyLight™ 800 770 794 1,050 IRDye®800, Alexa Fluor 790, Cy7™

 

High Fluorescence Intensity & Photostability

  • High-intensity emission provides outstanding sensitivity and requires less conjugate
  • Negligible bleed-through between fluorophore channels enables multicolor detection
  • Exceptional resistance to photobleaching
  • Buffers are completely stable at pH 4-9
  • DyLight™ is compatible with filter and laser settings on popular fluorescence instrumentation

 

DyLight™ 405 Conjugates

603-146-126
Chicken IgG (H&L) Antibody DyLight™ 405 Conjugated Pre-Adsorbed
3 References
Applications
IF, Multiplex
610-146-002
Mouse IgG (H&L) Antibody Dylight™ 405 Conjugated
3 References
Applications
IHC, IF, Multiplex
610-146-003
Mouse IgG F(c) Antibody DyLight™ 405 Conjugated
3 References
Applications
IHC, IF
611-146-002
Rabbit IgG (H&L) Antibody DyLight™ 405 Conjugated
2 References
Applications
IHC, IF

DyLight™ 488 Conjugates

600-141-215
GFP Antibody Dylight™ 488 Conjugated Pre-Adsorbed
23 References
Applications
WB, IHC, IF, FC, Dot Blot, IP, Multiplex
611-741-127
Rabbit IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed
9 References
Applications
WB, IHC, IF, Dot Blot, Multiplex
605-741-125
Goat IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed
7 References
Applications
IF, Dot Blot, Multiplex
610-741-124
Mouse IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed
6 References
Applications
WB, IHC, IF, Dot Blot, Multiplex

Dylight™ 549 Conjugates

611-142-002
Rabbit IgG (H&L) Antibody DyLight™ 549 Conjugated
8 References
Applications
WB, IF, Dot Blot, Multiplex
611-742-127
Rabbit IgG (H&L) Antibody DyLight™ 549 Conjugated Pre-Adsorbed
7 References
Applications
WB, IHC, IF, Dot Blot, Multiplex
600-442-378
V5 Epitope Tag Antibody Dylight™ 549 Conjugated
6 References
Applications
IF, EM, Multiplex
610-742-124
Mouse IgG (H&L) Antibody DyLight™ 549 Conjugated Pre-Adsorbed
6 References
Applications
WB, IF, Multiplex

DyLight™ 649 Conjugates

611-743-127
Rabbit IgG (H&L) Antibody DyLight™ 649 Conjugated Pre-Adsorbed
8 References
Applications
IHC, IF, Dot Blot, Multiplex
200-343-382
6X His Tag Antibody DyLight™ 649
5 References
Applications
WB, ELISA, Dot Blot, Biochemical Assay, Microarray
610-443-040
Mouse IgG1 (Gamma 1 chain) Antibody DyLight™ 649 Conjugated
6 References
Applications
Microarray
612-143-002
Rat IgG (H&L) Antibody Dylight™ 649 Conjugated
3 References
Applications
WB, IF, Multiplex

DyLight™ 680 Conjugates

611-144-002
Rabbit IgG (H&L) Antibody DyLight™ 680 Conjugated
35 References
Applications
WB, Dot Blot, IP
610-144-002
Mouse IgG (H&L) Antibody Dylight™ 680 Conjugated
30 References
Applications
WB, IHC, Dot Blot, IP
611-144-122
Rabbit IgG (H&L) Antibody DyLight™ 680 Conjugated Pre-Adsorbed
9 References
Applications
WB, Dot Blot, FISH, Multiplex
611-744-127
Rabbit IgG (H&L) Antibody DyLight™ 680 Conjugated Pre-Adsorbed
8 References
Applications
WB, Dot Blot

DyLight™ 800 Conjugates

611-145-002
Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated
67 References
Applications
WB, ELISA, IHC, Dot Blot, IP
610-145-002
Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated
57 References
Applications
WB, IHC, IF, Dot Blot
610-145-121
Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated Pre-Adsorbed
29 References
Applications
WB, IF, Dot Blot
611-145-122
Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated Pre-Adsorbed
22 References
Applications
WB, Dot Blot, Other