DyLight™ conjugated antibodies are high-performance fluorescent conjugates for use in primary or secondary antibody assays such as fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening, multiplex assays, and other array platforms. The antibodies are offered as highly functional conjugates with bright emission spectra that match the principal output wavelengths of common fluorescence instrumentation.
DyLight™ Fluorochromes
| Emission | Fluorochrome | Abs (nm) | Em (nm) | MW (daltons) | Spectrally similar |
| Blue | DyLight™ 405 | 420 | 400 | 793 | Alexa Fluor 350 |
| Green | DyLight™ 488 | 493 | 518 | 1,011 | Alexa Fluor 488, Fluorescein |
| Yellow | DyLight™ 549 | 550 | 568 | 982 | ATTO 532, Rhodamine, R-Phycoerythrin, Cy3™ |
| Red | DyLight™ 649 | 649 | 674 | 1,008 | ATTO 655, Allophycocyanin, Cy5™ |
| Near-infrared | DyLight™ 680 | 682 | 715 | 950 | IRDye®700, Alexa Fluor 680, Cy5.5™ |
| Infrared | DyLight™ 800 | 770 | 794 | 1,050 | IRDye®800, Alexa Fluor 790, Cy7™ |
High Fluorescence Intensity & Photostability
- High-intensity emission provides outstanding sensitivity and requires less conjugate
- Negligible bleed-through between fluorophore channels enables multicolor detection
- Exceptional resistance to photobleaching
- Buffers are completely stable at pH 4-9
- DyLight™ is compatible with filter and laser settings on popular fluorescence instrumentation
DyLight™ 405 Conjugates
603-146-126
3 References
Applications
IF, Multiplex
610-146-002
3 References
Applications
IHC, IF, Multiplex
DyLight™ 488 Conjugates
600-141-215
20 References
Applications
WB, IHC, IF, FC, Dot Blot, IP, Multiplex
611-741-127
8 References
Applications
WB, IHC, IF, Dot Blot, Multiplex
605-741-125
6 References
Applications
IF, Dot Blot, Multiplex
610-741-124
5 References
Applications
WB, IHC, IF, Dot Blot, Multiplex
Dylight™ 549 Conjugates
611-142-002
8 References
Applications
WB, IF, Dot Blot, Multiplex
611-742-127
7 References
Applications
WB, IHC, IF, Dot Blot, Multiplex
600-442-378
6 References
Applications
IF, EM, Multiplex
610-742-124
4 References
Applications
WB, IF, Multiplex
DyLight™ 649 Conjugates
611-743-127
6 References
Applications
IHC, IF, Dot Blot, Multiplex
200-343-382
5 References
Applications
WB, ELISA, Dot Blot, Biochemical Assay, Microarray
610-443-040
4 References
Applications
Microarray
612-143-002
3 References
Applications
WB, IF, Multiplex
DyLight™ 680 Conjugates
611-144-002
28 References
Applications
WB, Dot Blot, IP
610-144-002
27 References
Applications
WB, IHC, Dot Blot, IP
611-144-122
7 References
Applications
WB, Dot Blot, FISH, Multiplex
611-744-127
7 References
Applications
WB, Dot Blot
DyLight™ 800 Conjugates
611-145-002
50 References
Applications
WB, ELISA, IHC, Dot Blot, IP
610-145-002
46 References
Applications
WB, IHC, IF, Dot Blot
610-145-121
24 References
Applications
WB, IF, Dot Blot
611-145-122
18 References
Applications
WB, Dot Blot, Other