Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated
Mouse IgG Antibody DyLight 800 Conjugated - Dot Blot
Anti-Tubulin and Anti-TNFa Antibodies - Western Blot
DyLight™ 800 Fluorescence Spectra
Properties of DyLight™ Fluorescent Dyes.
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Datasheet

Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated

Goat Polyclonal

610-145-002 610-145-002-0.5
100 µg 5 x 100 µg
Dot Blot, WB, IF, IHC
Mouse
Goat
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$95.00 /Per Item
$162.00 /Per Item
100 µg $95.00
5 x 100 µg $162.00
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Anti-Mouse IgG DyLight 800 Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Note

Anti-Mouse IgG DyLight 800 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. This antibody will react with heavy chains of Mouse IgG and with light chains of most Mouse immunoglobulins.

Anti-MOUSE IgG (H&L) (GOAT) Antibody DyLight™ 800 Conjugated - 610-145-002
Goat Anti-Mouse IgG Secondary Antibody DyLight™800 Conjugated, Goat Anti-Mouse IgG Antibody DyLight™800 Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG DyLight™800 conjugated secondary antibody
Dot Blot, WB
IF, IHC
Mouse
Mouse
Goat
DyLight™ 800
Polyclonal
IgG
Mouse IgG whole molecule
2.7 moles DL800 per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Raimo S et al. Mitochondrial morphology, bioenergetics and proteomic responses in fatty acid oxidation disorders. Redox Biol. (2021)
Applications
WB, IB, PCA
Nitschke L et al. Modulation of ATXN1 S776 phosphorylation reveals the importance of allele-specific targeting in SCA1. JCS Insight. (2021)
Applications
WB, IB, PCA
De Giorgi M et al. Targeting the Apoa1 locus for liver-directed gene therapy. Mol Ther Methods Clin Dev. (2021)
Applications
WB, IB, PCA
Cuevas-Navarro A et al. The RAS GTPase RIT1 compromises mitotic fidelity through spindle assembly checkpoint suppression. Curr Biol. (2021)
Applications
WB, IB, PCA
Watt AC et al. CDK4/6 inhibition reprograms the breast cancer enhancer landscape by stimulating AP-1 transcriptional activity. Nat Cancer. (2021)
Applications
WB, IB, PCA
Zhang B et al. Dominant Role of PI3K p110α over p110β in Insulin and β-Adrenergic Receptor Signalling. Int J Mol Sci. (2021)
Applications
WB, IB, PCA
Nuebel E et al. The biochemical basis of mitochondrial dysfunction in Zellweger Spectrum Disorder. EMBO Rep. (2021)
Applications
Undefined
Woods BL. et al. Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling. bioRxiv (2020)
Applications
WB, IB, PCA
Kennedy T et al. Genetic background mutations drive neural circuit hyperconnectivity in a fragile X syndrome model. BMC Biol. (2020)
Applications
IF, Confocal Microscopy
Asthana V et al. Development of a Novel Class of Self-Assembling dsRNA Cancer Therapeutics: a Proof of Concept Investigation. Mol Ther Oncolytics. (2020)
Applications
WB, IB, PCA
Mitra S, Bodor DL, David AF, et al. Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin. Nat Commun. (2020)
Applications
WB, IB, PCA
Nowinski SM et al. Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria. Elife (2020)
Applications
WB, IB, PCA
Li R et al. RNF115 deletion inhibits autophagosome maturation and growth of gastric cancer Cell Death Dis. (2020)
Applications
WB, IB, PCA
Schutter M et al. Local fatty acid channeling into phospholipid synthesis drives phagophore expansion during autophagy. Cell. (2020)
Applications
WB, IB, PCA
Bensard CL et al. Regulation of tumor initiation by the mitochondrial pyruvate carrier. Cell Metab. (2020)
Applications
WB, IB, PCA
Zhan T et al. Cangrelor alleviates bleomycin-induced pulmonary fibrosis by inhibiting platelet activation in mice. Mol Immunol. (2020)
Applications
WB, IB, PCA
Bacman SR et al. Manipulation of mitochondrial genes and mtDNA heteroplasmy. Methods Cell Biol. (2020)
Applications
WB, IB, PCA
Ma X et al. Nicotinamide mononucleotide adenylyltransferase uses its NAD+ substrate-binding site to chaperone phosphorylated Tau. Elife. (2020)
Applications
WB, IB, PCA
Araiz et al. Enhanced β-adrenergic signalling underlies an age-dependent beneficial metabolic effect of PI3K p110α inactivation in adipose tissue. Nature Communications (2019)
Applications
WB, IB, PCA
Hong D et al. Deletion of TMEM268 inhibits growth of gastric cancer cells by downregulating the ITGB4 signaling pathway. Cell Death Differ. (2019)
Applications
WB, IB, PCA
Yu S et al. RSRC1 suppresses gastric cancer cell proliferation and migration by regulating PTEN expression. Mol Med Rep. (2019)
Applications
WB, IB, PCA
Wang M et al. Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization. J Vis Exp. (2019)
Applications
WB, IB, PCA
Bailetti AA et al. Enhancer of Polycomb and the Tip60 complex repress hematological tumor initiation by negatively regulating JAK/STAT pathway activity. Dis Model Mech. (2019)
Applications
WB, IB, PCA
Subramanian J et al. CPG15/neuritin mimics experience in selecting excitatory synapses for stabilization by facilitating PSD95 recruitment. Cell Rep. (2019)
Applications
WB, IB, PCA
Latorre-Pellicer A et al. Regulation of mother-to-offspring transmission of mtDNA heteroplasmy. Cell Metab. (2019)
Applications
WB, IB, PCA
Campbell LA et al. Gesicle-mediated delivery of CRISPR/Cas9 ribonucleoprotein complex for inactivating the HIV provirus. Mol Ther. (2019)
Applications
WB, IB, PCA
Lin et al. Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy. Cell Death & Disease (2018)
Applications
IHC, ICC, Histology; WB, IB, PCA
Zhang et al. Amelioratory Effects of Testosterone Propionate on Age-related Renal Fibrosis via Suppression of TGF-β1/Smad Signaling and Activation of Nrf2-ARE Signaling. Scientific Reports (2018)
Applications
WB, IB, PCA
Haapalainen et al. Expression of CPPED1 in human trophoblasts is associated with timing of term birth. Journal of Cellular and Molecular Medicine (2018)
Applications
WB, IB, PCA
Yu S et al. PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation. Int J Oncol. (2018)
Applications
WB, IB, PCA
Jarrett KE et al. Somatic Editing of Ldlr With Adeno-Associated Viral-CRISPR Is an Efficient Tool for Atherosclerosis Research. Arteriosclr Thromb Vasc Biol. (2018)
Applications
WB, IB, PCA
Zhan TW wt al. Cangrelor alleviates pulmonary fibrosis by inhibiting GPR17-mediated inflammation in mice. Int Immunopharmacol. (2018)
Applications
WB, IB, PCA
Bacman SR et al. MitoTALEN reduces mutant mtDNA load and restores tRNA Ala levels in a mouse model of heteroplasmic mtDNA mutation. Nat Med. (2018)
Applications
WB, IB, PCA
Jarrett et al. Somatic genome editing with CRISPR/Cas9 generates and corrects a metabolic disease. Scientific Reports (2017)
Applications
WB, IB, PCA
Li et al. Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality. Cell Death & Disease (2017)
Applications
WB, IB, PCA
Xia et al. Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress. Cell Death & Disease (2017)
Applications
WB, IB, PCA
Shen et al. EMC6/TMEM93 suppresses glioblastoma proliferation by modulating autophagy. Cell Death & Disease (2016)
Applications
WB, IB, PCA
Xia D et al. MARCH2 regulates autophagy by promoting CFTR ubiquitination and degradation and PIK3CA-AKT-MTOR signaling. Autophagy. (2016)
Applications
WB, IB, PCA
Wang Z et al. PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1. Autophagy. (2014)
Applications
IP, Co-IP
Li Y et al. A novel ER-localized transmembrane protein, EMC6, interacts with RAB5A and regulates cell autophagy. Autophagy. (2013)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.