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Background

Anti-Mouse IgG DyLight 800 Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Product Details

Goat Anti-Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated - 610-145-002
Goat Anti-Mouse IgG Secondary Antibody DyLight™800 Conjugated, Goat Anti-Mouse IgG Antibody DyLight™800 Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG DyLight™800 conjugated secondary antibody
Goat
IgG (H&L)
DyLight™ 800
Polyclonal
IgG
1.6

Target Details

Mouse
Mouse IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. This antibody will react with heavy chains of Mouse IgG and with light chains of most Mouse immunoglobulins.

Application Details

Dot Blot, WB
IF, IHC  - View References
Anti-Mouse IgG DyLight 800 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). Microglia Autophagy Mediated by TMEM166 Promotes Ischemic Stroke Secondary to Carotid Artery Stenosis. Aging Dis.
Applications
IF, Confocal Microscopy; WB, IB, PCA
(). Malaria parasite centrins can assemble by Ca2+-inducible condensation. PLoS P et al.
Applications
WB, IB, PCA
(). SARS-CoV-2 N protein induced acute kidney injury in diabetic db/db mice is associated with a Mincle-dependent M1 macrophage activation. Front Immunol.
Applications
WB, IB, PCA
(). Interleukin 6 (IL-6) Regulates GABAA Receptors in the Dorsomedial Hypothalamus Nucleus (DMH) through Activation of the JAK/STAT Pathway to Affect Heart Rate Variability in Stressed Rats. Int J Mol Sci.
Applications
WB, IB, PCA
(). Loss of respiratory complex I subunit NDUFB10 affects complex I assembly and supercomplex formation. Biol Chem.
Applications
WB, IB, PCA
(). The ribosomal S6 kinase 2 (RSK2)-SPRED2 complex regulates the phosphorylation of RSK substrates and MAPK signaling. J Biol Chem.
Applications
WB, IB, PCA
(). Parallel phospholipid transfer by Vps13 and Atg2 determines autophagosome biogenesis dynamics. J Cell Biol.
Applications
WB, IB, PCA
(). RAS-dependent RAF-MAPK hyperactivation by pathogenic RIT1 is a therapeutic target in Noonan syndrome-associated cardiac hypertrophy. Sci Adv.
Applications
WB, IB, PCA
(). Phenylbutyrate modulates polyamine acetylase and ameliorates Snyder-Robinson syndrome in a Drosophila model and patient cells. Jci Insight
Applications
WB, IB, PCA
(). STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer. Nature Communications
Applications
WB, IB, PCA
(). Reduction of mutant ATXN1 rescues premature death in a conditional SCA1 mouse model. JCI Insight
Applications
WB, IB, PCA
(). Intracerebral hemorrhage alters α2δ1 and thrombospondin expression in rats. Exp Ther Med.
Applications
WB, IB, PCA
(). LPA disruption with AAV-CRISPR potently lowers plasma apo(a) in transgenic mouse model: A proof-of-concept study. Mol Ther Methods Clin Dev.
Applications
WB, IB, PCA
(). Collateral deletion of the mitochondrial AAA+ ATPase ATAD1 sensitizes cancer cells to proteasome dysfunction. Elife.
Applications
WB, IB, PCA
(). Mitochondrial morphology, bioenergetics and proteomic responses in fatty acid oxidation disorders. Redox Biol.
Applications
WB, IB, PCA
(). Modulation of ATXN1 S776 phosphorylation reveals the importance of allele-specific targeting in SCA1. JCS Insight.
Applications
WB, IB, PCA
(). Targeting the Apoa1 locus for liver-directed gene therapy. Mol Ther Methods Clin Dev.
Applications
WB, IB, PCA
(). The RAS GTPase RIT1 compromises mitotic fidelity through spindle assembly checkpoint suppression. Curr Biol.
Applications
WB, IB, PCA
(). CDK4/6 inhibition reprograms the breast cancer enhancer landscape by stimulating AP-1 transcriptional activity. Nat Cancer.
Applications
WB, IB, PCA
(). Dominant Role of PI3K p110α over p110β in Insulin and β-Adrenergic Receptor Signalling. Int J Mol Sci.
Applications
WB, IB, PCA
(). The biochemical basis of mitochondrial dysfunction in Zellweger Spectrum Disorder. EMBO Rep.
Applications
Undefined
(). Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling. bioRxiv
Applications
WB, IB, PCA
(). TGF‐β‐induced IGFBP‐3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion. Mol Oncol.
Applications
WB, IB, PCA
(). Genetic background mutations drive neural circuit hyperconnectivity in a fragile X syndrome model. BMC Biol.
Applications
IF, Confocal Microscopy
(). Development of a Novel Class of Self-Assembling dsRNA Cancer Therapeutics: a Proof of Concept Investigation. Mol Ther Oncolytics.
Applications
WB, IB, PCA
(). Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin. Nat Commun.
Applications
WB, IB, PCA
(). Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria. Elife
Applications
WB, IB, PCA
(). RNF115 deletion inhibits autophagosome maturation and growth of gastric cancer Cell Death Dis.
Applications
WB, IB, PCA
(). Local fatty acid channeling into phospholipid synthesis drives phagophore expansion during autophagy. Cell.
Applications
WB, IB, PCA
(). Regulation of tumor initiation by the mitochondrial pyruvate carrier. Cell Metab.
Applications
WB, IB, PCA
(). Cangrelor alleviates bleomycin-induced pulmonary fibrosis by inhibiting platelet activation in mice. Mol Immunol.
Applications
WB, IB, PCA
(). Manipulation of mitochondrial genes and mtDNA heteroplasmy. Methods Cell Biol.
Applications
WB, IB, PCA
(). Nicotinamide mononucleotide adenylyltransferase uses its NAD+ substrate-binding site to chaperone phosphorylated Tau. Elife.
Applications
WB, IB, PCA
(). Enhanced β-adrenergic signalling underlies an age-dependent beneficial metabolic effect of PI3K p110α inactivation in adipose tissue. Nature Communications
Applications
WB, IB, PCA
(). Deletion of TMEM268 inhibits growth of gastric cancer cells by downregulating the ITGB4 signaling pathway. Cell Death Differ.
Applications
WB, IB, PCA
(). RSRC1 suppresses gastric cancer cell proliferation and migration by regulating PTEN expression. Mol Med Rep.
Applications
WB, IB, PCA
(). Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization. J Vis Exp.
Applications
WB, IB, PCA
(). Enhancer of Polycomb and the Tip60 complex repress hematological tumor initiation by negatively regulating JAK/STAT pathway activity. Dis Model Mech.
Applications
WB, IB, PCA
(). CPG15/neuritin mimics experience in selecting excitatory synapses for stabilization by facilitating PSD95 recruitment. Cell Rep.
Applications
WB, IB, PCA
(). Regulation of mother-to-offspring transmission of mtDNA heteroplasmy. Cell Metab.
Applications
WB, IB, PCA
(). Gesicle-mediated delivery of CRISPR/Cas9 ribonucleoprotein complex for inactivating the HIV provirus. Mol Ther.
Applications
WB, IB, PCA
(). Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy. Cell Death & Disease
Applications
IHC, ICC, Histology; WB, IB, PCA
(). Amelioratory Effects of Testosterone Propionate on Age-related Renal Fibrosis via Suppression of TGF-β1/Smad Signaling and Activation of Nrf2-ARE Signaling. Scientific Reports
Applications
WB, IB, PCA
(). Expression of CPPED1 in human trophoblasts is associated with timing of term birth. Journal of Cellular and Molecular Medicine
Applications
WB, IB, PCA
(). PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation. Int J Oncol.
Applications
WB, IB, PCA
(). Somatic Editing of Ldlr With Adeno-Associated Viral-CRISPR Is an Efficient Tool for Atherosclerosis Research. Arteriosclr Thromb Vasc Biol.
Applications
WB, IB, PCA
(). Cangrelor alleviates pulmonary fibrosis by inhibiting GPR17-mediated inflammation in mice. Int Immunopharmacol.
Applications
WB, IB, PCA
(). MitoTALEN reduces mutant mtDNA load and restores tRNA Ala levels in a mouse model of heteroplasmic mtDNA mutation. Nat Med.
Applications
WB, IB, PCA
(). MitoTALEN reduces mutant mtDNA load and restores tRNAAla levels in a mouse model of heteroplasmic mtDNA mutation. Nature Medicine
Applications
WB, IB, PCA
(). Somatic genome editing with CRISPR/Cas9 generates and corrects a metabolic disease. Scientific Reports
Applications
WB, IB, PCA
(). Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality. Cell Death & Disease
Applications
WB, IB, PCA
(). Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress. Cell Death & Disease
Applications
WB, IB, PCA
(). EMC6/TMEM93 suppresses glioblastoma proliferation by modulating autophagy. Cell Death & Disease
Applications
WB, IB, PCA
(). MARCH2 regulates autophagy by promoting CFTR ubiquitination and degradation and PIK3CA-AKT-MTOR signaling. Autophagy.
Applications
WB, IB, PCA
(). PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1. Autophagy.
Applications
IP, Co-IP
(). A novel ER-localized transmembrane protein, EMC6, interacts with RAB5A and regulates cell autophagy. Autophagy.
Applications
WB, IB, PCA
(). Early brain injury alters the blood-brain barrier phenotype in parallel with β-amyloid and cognitive changes in adulthood. J Cereb Blood Flow Metab.
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.