Chicken IgG (H&L) Antibody DyLight™ 405 Conjugated Pre-Adsorbed
Western Blot of Unconjugated Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins)
DyLight™ 405 Fluorescence absorption and emission spectra in PBS, pH 7.2.
Properties of DyLight™ Fluorescent Dyes.
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Datasheet

Chicken IgG (H&L) Antibody DyLight™ 405 Conjugated Pre-Adsorbed

Goat Polyclonal

603-146-126
100 µg
IF
Goat
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Description

Background

Anti-Chicken IgG DyLight Antibody generated in goat detects chicken IgY. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Application Note

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Conjugated Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purity/Specificity

Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum.  No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.  This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.

Anti-CHICKEN IgG (H&L) (GOAT) Antibody DyLight™ 405 Conjugated (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) - 603-146-126
goat anti-Chicken IgG DyLight™405 Conjugated Antibody, goat anti-Chicken IgG Antibody DyLight™405 Conjugation, Chicken Secondary Antibody, goat anti-Chicken IgY DyLight™405
IF
Chicken
Goat
DyLight™ 405
Polyclonal
IgG
Chicken IgG whole molecule
2.0 moles DL405 per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Gottle P et al. Rescuing the negative impact of human endogenous retrovirus envelope protein on oligodendroglial differentiation and myelination. Glia. (2019)
Applications
IF, Confocal Microscopy
Göttle et al. Teriflunomide promotes oligodendroglial differentiation and myelination. Journal of Neuroinflammation (2018)
Applications
IF, Confocal Microscopy
Göttle et al. Oligodendroglial maturation is dependent on intracellular protein shuttling. The Journal of Neuroscience (2015)
Applications
IF, Confocal Microscopy

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.