Mouse IgG (H&L) Antibody Peroxidase Conjugated
Western Blot - Mouse IgG HRP Conjugated
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Datasheet

Mouse IgG (H&L) Antibody Peroxidase Conjugated

Goat Polyclonal

610-1302
2 mg
ELISA, WB, IHC, Other
Mouse
Goat
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$141.00 /Per Item
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Description

Background

Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. To enable detection, the secondary antibody must have specificity for the antibody species and isotype of the primary antibody being used and generally is conjugated. Rockland produces highly active antibodies and conjugates against mouse immunoglobulins. Anti-Mouse Secondary Antibodies are affinity-purified polyclonal antibodies with well-characterized specificity for mouse immunoglobulin classes, subclasses, and fragments. They are available as unlabeled antibodies as well as conjugates of alkaline phosphatase (AP), horseradish peroxidase (HRP), fluorescent conjugates, and biotin. This Anti-MOUSE IgG generated in goat is designed to detect heavy and light chains (H&L) and has been Peroxidase (HRP) conjugated. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays. These secondary antibodies are used throughout various types of assays, including ELISA or Western Blot, Immunohistochemistry, Flow Cytometry. Optimal secondary antibody requires knowledge of the detection assay.

Application Note

Anti-Mouse secondary antibody conjugated to horseradish peroxidase (HRP) generated in goat detects specifically Mouse IgG whole molecule. This anti-Mouse HRP antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Purity/Specificity

MOUSE IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.

Anti-MOUSE IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 610-1302
Goat Anti-Mouse IgG Secondary Antibody Peroxidase Conjugated, Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated, GAM-HRP, Anti-mouse secondary antibody, anti-mouse HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-mouse horseradish peroxidase conjugated secondary antibody
ELISA, WB
IHC, Other
Mouse
Goat
IgG (H&L)
Peroxidase (Horseradish)
Polyclonal
IgG
Native Protein
Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.
Lyophilized
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store HRP Conjugated Mouse Secondary Antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Strohmeier, S et al. A CpG 1018 adjuvanted neuraminidase vaccine provides robust protection from influenza virus challenge in mice. Npj Vaccines (2022)
Applications
EIA, ELISA
Hormazabal J et al. Chaperone mediated autophagy contributes to the newly synthesized histones H3 and H4 quality control. Nucleic Acids Res. (2022)
Applications
WB, IB, PCA
Ortega L et al. The Ω-3 fatty acid docosahexaenoic acid selectively induces apoptosis in tumor-derived cells and suppress tumor growth in gastric cancer. Eur J Pharmacol. (2021)
Applications
Undefined
Carreno JM et al. Evidence for retained spike-binding and neutralizing activity against emerging SARS-CoV-2 variants in serum of COVID-19 mRNA vaccine recipients. EBioMedicine (2021)
Applications
Microneutralization Assay (MNT)
Arunkumar GA et al. Functionality of the putative surface glycoproteins of the Wuhan spiny eel influenza virus. Nat Commun. (2021)
Applications
EIA, ELISA
Yang X, He Z, Zhang G, et al. Evaluation of Reactivity of Monoclonal Antibodies Against Omp25 of Brucella spp. Front Cell Infect Microbiol. (2020)
Applications
IHC, ICC, Histology
Amanat F, Stadlbauer D, Strohmeier S, et al. A serological assay to detect SARS-CoV-2 seroconversion in humans. Nat. Med. (2020)
Applications
NEU
Ramirez DH, Aonbangkhen C, Wu HY, et al. Engineering a Proximity-Directed O-GlcNAc Transferase for Selective Protein O-GlcNAcylation in Cells. ACS Chem Biol. (2020)
Applications
WB, IB, PCA
Gallardo et al. Effect of Breed on Transcriptional and Protein Expression of Lipogenic Enzymes in Tail and Subcutaneous Adipose Tissue from Two Grazing Breeds of Lambs. Animals (2019)
Applications
WB, IB, PCA
Gerber KJ, Squires KE, Hepler JR. 14-3-3γ binds regulator of G protein signaling 14 (RGS14) at distinct sites to inhibit the RGS14:Gαi-AlF4- signaling complex and RGS14 nuclear localization. J Biol Chem. (2019)
Applications
WB, IB, PCA
Jiang S, Xu Y. Annexin A2 upregulation protects human retinal endothelial cells from oxygen-glucose deprivation injury by activating autophagy. Exp Ther Med. (2019)
Applications
WB, IB, PCA
Noppes S, Müller SF, Bennien J, et al. Homo- and heterodimerization is a common feature of the solute carrier family SLC10 members. Biol Chem. (2019)
Applications
Undefined
Wang XX, Zhang H, Li Y. Preliminary study on the role of miR‑148a and DNMT1 in the pathogenesis of acute myeloid leukemia. Mol Med Rep. (2019)
Applications
WB, IB, PCA
Henninger C, Pohlmann S, Ziegler V, Ohlig J, Schmitt J, Fritz G. Distinct contribution of Rac1 expression in cardiomyocytes to anthracycline-induced cardiac injury. Biochem Pharmacol. (2019)
Applications
Undefined
Sanderson SM, Mikhael PG, Ramesh V, Dai Z, Locasale JW. Nutrient availability shapes methionine metabolism in p16/MTAP-deleted cells. Sci Adv. (2019)
Applications
WB, IB, PCA
Lin N, Dong XJ, Wang TY, et al. Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841). Mol Med Rep. (2019)
Applications
WB, IB, PCA
Fegan, JE et al. Utility of Hybrid Transferrin Binding Protein Antigens for Protection Against Pathogenic Neisseria Species. Frontiers in Immunology (2019)
Applications
EIA, ELISA
Elsaeidi et al. Notch Suppression Collaborates with Ascl1 and Lin28 to Unleash a Regenerative Response in Fish Retina, But Not in Mice. The Journal of Neuroscience (2018)
Applications
WB, IB, PCA
Spasojevic et al. PKD1 is a potential biomarker and therapeutic target in triple-negative breast cancer. Oncotarget (2018)
Applications
WB, IB, PCA
Ray et al. Regulation of Trypanosoma brucei Acetyl Coenzyme A Carboxylase by Environmental Lipids. mSphere (2018)
Applications
WB, IB, PCA
Rahman MT, Ghosh C, Hossain M, et al. IFN-γ, IL-17A, or zonulin rapidly increase the permeability of the blood-brain and small intestinal epithelial barriers: Relevance for neuro-inflammatory diseases. Biochem Biophys Res Commun. (2018)
Applications
WB, IB, PCA
Liberti et al. A Predictive Model for Selective Targeting of the Warburg Effect through GAPDH Inhibition with a Natural Product. Cell Metabolism (2017)
Applications
WB, IB, PCA
Ciesielska A et al. Bis (monoacylglycero) phosphate inhibits TLR4-dependent RANTES production in macrophages. Int J Biochem Cell Biol. (2017)
Applications
Undefined
Neiser et al. Assessment of Dextran Antigenicity of Intravenous Iron Preparations with Enzyme-Linked Immunosorbent Assay (ELISA). International Journal of Molecular Sciences (2016)
Applications
E, EIA
Mendez et al. Endoplasmic reticulum stress-independent activation of unfolded protein response kinases by a small molecule ATP-mimic. Elife (2015)
Applications
WB, IB, PCA
Turner, NA et al. Factor VIII Is Synthesized in Human Endothelial Cells, Packaged in Weibel-Palade Bodies and Secreted Bound to ULVWF Strings. PloS One (2015)
Applications
WB, IB, PCA
Ohta H, Sunden Y, Yokoyama N, et al. Expression of apical junction complex proteins in duodenal mucosa of dogs with inflammatory bowel disease. Am J Vet Res. (2014)
Applications
WB, IB, PCA
Xu S, Grande F, Garofalo A, Neamati N. Discovery of a novel orally active small-molecule gp130 inhibitor for the treatment of ovarian cancer. Mol Cancer Ther. (2013)
Applications
WB, IB, PCA
Junk DJ, Cipriano R, Stampfer M, Jackson MW. Constitutive CCND1/CDK2 activity substitutes for p53 loss, or MYC or oncogenic RAS expression in the transformation of human mammary epithelial cells. PLoS One. (2013)
Applications
WB, IB, PCA
Horn P et al. Circulating microparticles carry a functional endothelial nitric oxide synthase that is decreased in patients with endothelial dysfunction. J Am Heart Assoc. (2012)
Applications
WB, IB, PCA
Zong M, Satoh A, Yu MK, et al. TRAPPC9 mediates the interaction between p150 and COPII vesicles at the target membrane. PLoS One. (2012)
Applications
WB, IB, PCA
Den Herder G, Yoshida S, Antolín-Llovera M, Ried MK, Parniske M. Lotus japonicus E3 ligase SEVEN IN ABSENTIA4 destabilizes the symbiosis receptor-like kinase SYMRK and negatively regulates rhizobial infection. Plant Cell. (2012)
Applications
WB, IB, PCA
Pullen NA, Anand M, Cooper PS, Fillmore HL. Matrix metalloproteinase-1 expression enhances tumorigenicity as well as tumor-related angiogenesis and is inversely associated with TIMP-4 expression in a model of glioblastoma. J Neurooncol. (2012)
Applications
Undefined
LeVine H et al. Dihydroxybenzoic acid isomers differentially dissociate soluble biotinyl-Aβ(1-42) oligomers. Biochemistry (2012)
Applications
E, EIA
Ohta H, Yamaguchi T, Rajapakshage BK, et al. Expression and subcellular localization of apical junction proteins in canine duodenal and colonic mucosa. Am J Vet Res. (2011)
Applications
WB, IB, PCA
Zhang X et al. Vaccination with different M2e epitope densities confers partial protection against H5N1 influenza A virus challenge in chickens. Intervirology. (2011)
Applications
WB, IB, PCA
McCall LI et al. Localization and induction of the A2 virulence factor in Leishmania: evidence that A2 is a stress response protein. Mol Microbiol. (2010)
Applications
WB, IB, PCA
Reeves TM et al. Proteolysis of submembrane cytoskeletal proteins ankyrin-G and αII-spectrin following diffuse brain injury: a role in white matter vulnerability at Nodes of Ranvier. Brain Pathology (Zurich, Switzerland) (2010)
Applications
WB, IB, PCA
Pullen NA, Fillmore HL. Induction of matrix metalloproteinase-1 and glioma cell motility by nitric oxide. J Neurooncol. (2010)
Applications
Undefined
Mafra D, Santos FR, Lobo JC, et al. Alpha-tocopherol supplementation decreases electronegative low-density lipoprotein concentration [LDL(-)] in haemodialysis patients. Nephrol Dial Transplant. (2009)
Applications
E, EIA
Lobo J, Santos F, Grosso D, et al. Electronegative LDL and lipid abnormalities in patients undergoing hemodialysis and peritoneal dialysis. Nephron Clin Pract. (2008)
Applications
WB, IB, PCA; E, EIA
Oliveira JA, Sevanian A, Rodrigues RJ, Apolinário E, Abdalla DS. Minimally modified electronegative LDL and its autoantibodies in acute and chronic coronary syndromes. Clin Biochem. (2006)
Applications
E, EIA
Van Komen JS et al. The polybasic juxtamembrane region of Sso1p is required for SNARE function in vivo. Eukaryot Cell. (2005)
Applications
WB, IB, PCA
Dennison AC, Van Metre DC, Morley PS, Callan RJ, Plampin EC, Ellis RP. Comparison of the odds of isolation, genotypes, and in vivo production of major toxins by Clostridium perfringens obtained from the gastrointestinal tract of dairy cows with hemorrhagic bowel syndrome or left-displaced abomasum. J Am Vet Med Assoc. (2005)
Applications
E, EIA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.