Background

Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. To enable detection, the secondary antibody must have specificity for the antibody species and isotype of the primary antibody being used and generally is conjugated. Rockland produces highly active antibodies and conjugates against mouse immunoglobulins. Anti-Mouse Secondary Antibodies are affinity-purified polyclonal antibodies with well-characterized specificity for mouse immunoglobulin classes, subclasses, and fragments. They are available as unlabeled antibodies as well as conjugates of alkaline phosphatase (AP), horseradish peroxidase (HRP), fluorescent conjugates, and biotin. This Anti-MOUSE IgG generated in goat is designed to detect heavy and light chains (H&L) and has been Peroxidase (HRP) conjugated. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays. These secondary antibodies are used throughout various types of assays, including ELISA or Western Blot, Immunohistochemistry, Flow Cytometry. Optimal secondary antibody requires knowledge of the detection assay.

Product Details

Goat Anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated - 610-1302
Goat Anti-Mouse IgG Secondary Antibody Peroxidase Conjugated, Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated, GAM-HRP, Anti-mouse secondary antibody, anti-mouse HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-mouse horseradish peroxidase conjugated secondary antibody
Goat
IgG (H&L)
Peroxidase (Horseradish)
Polyclonal
IgG

Target Details

Mouse
Native Protein
Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.
MOUSE IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.

Application Details

ELISA, WB
IHC, Other  - View References
Anti-Mouse secondary antibody conjugated to horseradish peroxidase (HRP) generated in goat detects specifically Mouse IgG whole molecule. This anti-Mouse HRP antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation

Lyophilized
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store HRP Conjugated Mouse Secondary Antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Coordination of bacterial cell wall and outer membrane biosynthesis. Nature.
Applications
WB, IB, PCA
(). Redistribution of the Novel Clostridioides difficile Spore Adherence Receptor E-Cadherin by TcdA and TcdB Increases Spore Binding to Adherens Junctions. Infect Immun.
Applications
EIA, ELISA; WB, IB, PCA
(). Co-culture models of endothelial cells, macrophages, and vascular smooth muscle cells for the study of the natural history of atherosclerosis. PLoS One.
Applications
WB, IB, PCA
(). Neurogenin 2 and Neuronal Differentiation 1 Control Proper Development of the Chick Trigeminal Ganglion and Its Nerve Branches. J Dev Biol.
Applications
WB, IB, PCA
(). Neural crest cell-placodal neuron interactions are mediated by Cadherin-7 and N-cadherin during early chick trigeminal ganglion assembly. F1000Res.
Applications
WB, IB, PCA
(). A CpG 1018 adjuvanted neuraminidase vaccine provides robust protection from influenza virus challenge in mice. Npj Vaccines
Applications
EIA, ELISA
(). Chaperone mediated autophagy contributes to the newly synthesized histones H3 and H4 quality control. Nucleic Acids Res.
Applications
WB, IB, PCA
(). The Ω-3 fatty acid docosahexaenoic acid selectively induces apoptosis in tumor-derived cells and suppress tumor growth in gastric cancer. Eur J Pharmacol.
Applications
Undefined
(). The Ω-3 fatty acid docosahexaenoic acid selectively induces apoptosis in tumor-derived cells and suppress tumor growth in gastric cancer. Eur J Pharmacol.
Applications
WB, IB, PCA
(). Evidence for retained spike-binding and neutralizing activity against emerging SARS-CoV-2 variants in serum of COVID-19 mRNA vaccine recipients. EBioMedicine
Applications
Microneutralization Assay (MNT)
(). Functionality of the putative surface glycoproteins of the Wuhan spiny eel influenza virus. Nat Commun.
Applications
EIA, ELISA
(). Evaluation of Reactivity of Monoclonal Antibodies Against Omp25 of Brucella spp. Front Cell Infect Microbiol.
Applications
IHC, ICC, Histology
(). A serological assay to detect SARS-CoV-2 seroconversion in humans. Nat. Med.
Applications
NEU
(). Engineering a Proximity-Directed O-GlcNAc Transferase for Selective Protein O-GlcNAcylation in Cells. ACS Chem Biol.
Applications
WB, IB, PCA
(). Effect of Breed on Transcriptional and Protein Expression of Lipogenic Enzymes in Tail and Subcutaneous Adipose Tissue from Two Grazing Breeds of Lambs. Animals
Applications
WB, IB, PCA
(). 14-3-3γ binds regulator of G protein signaling 14 (RGS14) at distinct sites to inhibit the RGS14:Gαi-AlF4- signaling complex and RGS14 nuclear localization. J Biol Chem.
Applications
WB, IB, PCA
(). Annexin A2 upregulation protects human retinal endothelial cells from oxygen-glucose deprivation injury by activating autophagy. Exp Ther Med.
Applications
WB, IB, PCA
(). Homo- and heterodimerization is a common feature of the solute carrier family SLC10 members. Biol Chem.
Applications
WB, IB, PCA
(). Preliminary study on the role of miR‑148a and DNMT1 in the pathogenesis of acute myeloid leukemia. Mol Med Rep.
Applications
WB, IB, PCA
(). Distinct contribution of Rac1 expression in cardiomyocytes to anthracycline-induced cardiac injury. Biochem Pharmacol.
Applications
IF, Confocal Microscopy
(). Nutrient availability shapes methionine metabolism in p16/MTAP-deleted cells. Sci Adv.
Applications
WB, IB, PCA
(). Characteristics of olfactory ensheathing cells and microarray analysis in Tupaia belangeri (Wagner, 1841). Mol Med Rep.
Applications
WB, IB, PCA
(). Utility of Hybrid Transferrin Binding Protein Antigens for Protection Against Pathogenic Neisseria Species. Frontiers in Immunology
Applications
EIA, ELISA
(). Notch Suppression Collaborates with Ascl1 and Lin28 to Unleash a Regenerative Response in Fish Retina, But Not in Mice. The Journal of Neuroscience
Applications
WB, IB, PCA
(). PKD1 is a potential biomarker and therapeutic target in triple-negative breast cancer. Oncotarget
Applications
WB, IB, PCA
(). Regulation of Trypanosoma brucei Acetyl Coenzyme A Carboxylase by Environmental Lipids. mSphere
Applications
WB, IB, PCA
(). IFN-γ, IL-17A, or zonulin rapidly increase the permeability of the blood-brain and small intestinal epithelial barriers: Relevance for neuro-inflammatory diseases. Biochem Biophys Res Commun.
Applications
WB, IB, PCA
(). A Predictive Model for Selective Targeting of the Warburg Effect through GAPDH Inhibition with a Natural Product. Cell Metabolism
Applications
WB, IB, PCA
(). Bis (monoacylglycero) phosphate inhibits TLR4-dependent RANTES production in macrophages. Int J Biochem Cell Biol.
Applications
EIA, ELISA
(). Assessment of Dextran Antigenicity of Intravenous Iron Preparations with Enzyme-Linked Immunosorbent Assay (ELISA). International Journal of Molecular Sciences
Applications
E, EIA
(). Frataxin silencing alters microtubule stability in motor neurons: implications for Friedreich's ataxia. Hum Mol Genet.
Applications
WB, IB, PCA
(). Endoplasmic reticulum stress-independent activation of unfolded protein response kinases by a small molecule ATP-mimic. Elife
Applications
WB, IB, PCA
(). Factor VIII Is Synthesized in Human Endothelial Cells, Packaged in Weibel-Palade Bodies and Secreted Bound to ULVWF Strings. PloS One
Applications
WB, IB, PCA
(). Expression of apical junction complex proteins in duodenal mucosa of dogs with inflammatory bowel disease. Am J Vet Res.
Applications
WB, IB, PCA
(). Discovery of a novel orally active small-molecule gp130 inhibitor for the treatment of ovarian cancer. Mol Cancer Ther.
Applications
WB, IB, PCA
(). Constitutive CCND1/CDK2 activity substitutes for p53 loss, or MYC or oncogenic RAS expression in the transformation of human mammary epithelial cells. PLoS One.
Applications
WB, IB, PCA
(). Circulating microparticles carry a functional endothelial nitric oxide synthase that is decreased in patients with endothelial dysfunction. J Am Heart Assoc.
Applications
WB, IB, PCA
(). TRAPPC9 mediates the interaction between p150 and COPII vesicles at the target membrane. PLoS One.
Applications
WB, IB, PCA
(). Lotus japonicus E3 ligase SEVEN IN ABSENTIA4 destabilizes the symbiosis receptor-like kinase SYMRK and negatively regulates rhizobial infection. Plant Cell.
Applications
WB, IB, PCA
(). Matrix metalloproteinase-1 expression enhances tumorigenicity as well as tumor-related angiogenesis and is inversely associated with TIMP-4 expression in a model of glioblastoma. J Neurooncol.
Applications
WB, IB, PCA
(). Dihydroxybenzoic acid isomers differentially dissociate soluble biotinyl-Aβ(1-42) oligomers. Biochemistry
Applications
E, EIA
(). Expression and subcellular localization of apical junction proteins in canine duodenal and colonic mucosa. Am J Vet Res.
Applications
WB, IB, PCA
(). Vaccination with different M2e epitope densities confers partial protection against H5N1 influenza A virus challenge in chickens. Intervirology.
Applications
WB, IB, PCA
(). Localization and induction of the A2 virulence factor in Leishmania: evidence that A2 is a stress response protein. Mol Microbiol.
Applications
WB, IB, PCA
(). Proteolysis of submembrane cytoskeletal proteins ankyrin-G and αII-spectrin following diffuse brain injury: a role in white matter vulnerability at Nodes of Ranvier. Brain Pathology (Zurich, Switzerland)
Applications
WB, IB, PCA
(). Induction of matrix metalloproteinase-1 and glioma cell motility by nitric oxide. J Neurooncol.
Applications
WB, IB, PCA
(). Alpha-tocopherol supplementation decreases electronegative low-density lipoprotein concentration [LDL(-)] in haemodialysis patients. Nephrol Dial Transplant.
Applications
E, EIA
(). Electronegative LDL and lipid abnormalities in patients undergoing hemodialysis and peritoneal dialysis. Nephron Clin Pract.
Applications
WB, IB, PCA; E, EIA
(). Minimally modified electronegative LDL and its autoantibodies in acute and chronic coronary syndromes. Clin Biochem.
Applications
E, EIA
(). The polybasic juxtamembrane region of Sso1p is required for SNARE function in vivo. Eukaryot Cell.
Applications
WB, IB, PCA
(). Comparison of the odds of isolation, genotypes, and in vivo production of major toxins by Clostridium perfringens obtained from the gastrointestinal tract of dairy cows with hemorrhagic bowel syndrome or left-displaced abomasum. J Am Vet Med Assoc.
Applications
E, EIA

Invalid lot number

If you need help finding your CoA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.