Mouse IgG [H&L] Antibody CY5 Conjugated Pre-adsorbed
WBM - MOUSE IgG [H&L] Antibody CY5 Conjugated Pre-adsorbed
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Datasheet

Mouse IgG [H&L] Antibody CY5 Conjugated Pre-adsorbed

Goat Polyclonal

610-110-121
1 mg
Dot Blot, ELISA, WB, FC, IF
Goat
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$260.00 /Per Item
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Description

Background

Anti-Mouse IgG Cy5 Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Note

Anti-Mouse IgG Cy5 Antibody has been tested by ELISA, dot blot, and western blot and is designed for flow cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purity/Specificity

Goat anti-Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rat, Rabbit and Sheep Serum Proteins.

Storage Condition

Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Anti-MOUSE IgG [H&L] (GOAT) Antibody CY5 Conjugated Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins - 610-110-121
Goat Anti-Mouse IgG (H&L) Antibody CY5 Conjugated Pre-Adsorbed, Goat Anti Mouse IgG Antibody CY5 Conjugated, Cy5 anti-Mouse IgG
Dot Blot, ELISA, WB
FC, IF
Mouse
Goat
Cy5™
Polyclonal
IgG
12.6 moles CY5 per mole of IgG
Lyophilized
Ambient
Expiration date is one (1) year from date of opening.
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Restore with deionized water (or equivalent)
1.0 mL
Gomes et al. Proteomic analysis of physiological versus pathological cardiac remodeling in animal models expressing mutations in myosin essential light chains. (2015)
Applications
WB, IB, PCA
Markoullis K et al. Mycoplasma contamination of murine embryonic stem cells affects cell parameters, germline transmission and chimeric progeny. (2009)
Applications
IF, Confocal Microscopy
Achour L et al. CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface. (2009)
Applications
FC, FACS, FLOW

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If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.