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Background

Anti-Mouse IgG Alkaline Phosphatase Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Product Details

Goat Anti-Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated - 610-1502
Goat Anti-Mouse IgG Antibody alkaline phosphatase Conjugation, Goat Anti-Mouse IgG alk phos Conjugated Antibody
Goat
IgG (H&L)
Alkaline Phosphatase (Calf Intestine)
Polyclonal
IgG

Target Details

Mouse
Mouse IgG whole molecule
Anti-Mouse Conjugated Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse IgG and Mouse Serum.

Application Details

Dot Blot, ELISA, WB
IF, LFA  - View References
Mouse secondary antibody conjugated to Alkaline Phosphatase is available in a variety of formats. Anti-Mouse IgG Alkaline Phosphatase Antibody has been tested by ELISA, dot blot, and Western blot and is suitable for ELISA, immunohistochemistry, Western blotting as well as other Alkaline Phosphatase antibody based assays.

Formulation

Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
0.1% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Shipping & Handling

Wet Ice
Store secondary antibody conjugate at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Expiration date is one (1) year from date of receipt.
(). Development of a lateral flow assay for the detection of amitriptyline in surface waters 14th Dresden Sensor Symposium 2019
Applications
Lateral Flow (LFA)
(). Molecular characterization and expression analysis of cDNAs encoding four Rab and two Arf GTPases in the latex of Hevea brasiliensis. Plant Physiol Biochem.
Applications
WB, IB, PCA
(). Ebola virus VP24 binds karyopherin α1 and blocks STAT1 nuclear accumulation. J Virol.
Applications
IF, Confocal Microscopy; WB, IB, PCA
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Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.