Mouse IgG (H&L) Antibody ATTO 488 Conjugated Pre-Adsorbed
Atto Dye-Immunofluorescence microscopy
WBM - Mouse IgG (H&L) Antibody ATTO 488 Conjugated Pre-Adsorbed
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Datasheet

Mouse IgG (H&L) Antibody ATTO 488 Conjugated Pre-Adsorbed

Goat Polyclonal

610-152-121S 610-152-121
100 µg 500 µg
Dot Blot, WB
Mouse
Goat
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$99.00 /Per Item
$332.00 /Per Item
100 µg $99.00
500 µg $332.00
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Description

Background

Anti-Mouse IgG ATTO 488 Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Note

Anti-Mouse IgG ATTO 488 Antibody has been tested by dot blot and western blot. Anti-Mouse IgG (H&L) conjugated by ATTO 488 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Purity/Specificity

Mouse IgG (H&L) Antibody ATTO 488 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.  This antibody will react with heavy chains of mouse IgG and with light chains of most mouse immunoglobulins.

Anti-MOUSE IgG (H&L) (GOAT) Antibody ATTO 488 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) - 610-152-121
Goat Anti-Mouse IgG Antibody ATTO488 Conjugation, Goat Anti-Mouse IgG ATTO 488 Conjugated Antibody
Dot Blot, WB
Mouse
Goat
IgG (H&L)
ATTO 488
Polyclonal
IgG
Mouse IgG whole molecule
3.8 moles AT488 per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
500 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
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Applications
IF, Confocal Microscopy
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Applications
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Scott, WA et al. ATRX proximal protein associations boast roles beyond histone deposition. PloS Genetics (2021)
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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.