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Datasheet Protocol SDS

Rabbit TrueBlot®: Anti-Rabbit IgG HRP

Mouse Monoclonal eB182

314 References
18-8816-31 18-8816-33
50 µL 200 µL
Liquid (sterile filtered) Liquid (sterile filtered)
WB, ELISA, IF, IP, ChIP, FISH
Rabbit
Mouse
$129.00 /Per Item
$260.00 /Per Item
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$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated Anti-Rabbit IgG monoclonal secondary antibody. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Rabbit IgG TrueBlot®, simply substitute the conventional HRP Anti-Rabbit IgG blotting reagent with Rabbit IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Rabbit IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.

Product Details

Rabbit TrueBlot®: Anti-Rabbit IgG HRP - 18-8816-31
Anti-Rabbit IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
Mouse
Peroxidase (HRP) ULTRA
Monoclonal
IgG
Immunoprecipitation Kit

Target Details

Rabbit
Rabbit TrueBlot® Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Rabbit Serum. Reactivity is observed against native Rabbit IgG by both Western blot and ELISA.

Application Details

ELISA, IP, WB
ChIP, FISH, IF  - View References
Rabbit IgG HRP TrueBlot has been tested in ELISA, Western blot, and immunoprecipitation and may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Rabbit IgG TrueBlot® is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5 mLs/blot will yield enough reagent for 20 blots. Note that there are three key procedural considerations: 1. Protein A or G should not be used for the immunoprecipitation. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands. For immunoprecipitation, Anti-Rat IgG beads, or Anti-Rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Proclin is added as an antimicrobial agent.
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Shipping & Handling

Wet Ice
Store at -20°C. This product is guaranteed for 6 months upon receipt, when handled and stored as instructed.
Expiration date is six (6) months from date of receipt.
(). Constitutive KCC2 Cell- and Synapse-Specifically Regulates NMDA Receptor Activity in the Spinal Cord. J Neurosci.
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WB, IB, PCA
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(). Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders. J Clin Invest.
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(). Activation of innate immune receptor TLR9 by mitochondrial DNA plays essential roles in the chemical long-term depression of hippocampal neurons. J Biol Chem.
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WB, IB, PCA
(). Inhibition of autocrine HGF maturation overcomes cetuximab resistance in colorectal cancer. Cell Mol Life Sci.
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(). AMPKα2 regulates fasting-induced hyperketonemia by suppressing SCOT ubiquitination and degradation. Sci Rep.
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(). KDM6A Loss Triggers an Epigenetic Switch That Disrupts Urothelial Differentiation and Drives Cell Proliferation in Bladder Cancer. Cancer Res.
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(). mGluR5 from Primary Sensory Neurons Promotes Opioid-Induced Hyperalgesia and Tolerance by Interacting with and Potentiating Synaptic NMDA Receptors. J Neurosci.
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(). Hepatocyte FBXW7-dependent activity of nutrient-sensing nuclear receptors controls systemic energy homeostasis and NASH progression in male mice. Nat Commun.
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(). Direct and biologically significant interactions of human herpesvirus 8 interferon regulatory factor 1 with STAT3 and Janus kinase TYK2. PLoS Pathog.
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(). Nucleolus activity-dependent recruitment and biomolecular condensation by pH sensing. Mol Cell.
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(). SMAD3 promotes expression and activity of the androgen receptor in prostate cancer. Nucleic Acids Res.
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(). The tyrosine phosphatase LAR acts as a receptor of the nidogen-tetanus toxin complex. bioRxiv Preprint
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(). α2δ‐1 protein drives opioid‐induced conditioned reward and synaptic NMDA receptor hyperactivity in the nucleus accumbens. J Neurochem.
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Undefined
(). Deubiquitinase catalytic activity of MYSM1 is essential in vivo for hematopoiesis and immune cell development. Sci Rep.
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WB, IB, PCA
(). MDC1 maintains active elongation complexes of RNA polymerase II. Cell Rep.
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WB, IB, PCA
(). Hyperactivation of YAP/TAZ drives alterations in mesangial cells through stabilization of N-MYC in diabetic nephropathy. J Am Soc Nephrol.
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IP, Co-IP
(). AKT Phosphorylates FAM13A and Promotes Its Degradation via CUL4A/DDB1/DCAF1 E3 Complex. Am J Repair Cell Mol Biol.
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WB, IB, PCA
(). A role for the Gram-negative outer membrane in bacterial shape determination. bioRxiv Preprint.
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WB, IB, PCA
(). RNA Helicase DDX6 Regulates A-to-I Editing and Neuronal Differentiation in Human Cells. Int J Mol Sci.
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WB, IB, PCA
(). Coordination of bacterial cell wall and outer membrane biosynthesis. Nature.
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IP, Co-IP; WB, IB, PCA
(). The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11. Cell Rep.
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WB, IB, PCA
(). HNRNPU facilitates antibody class-switch recombination through C-NHEJ promotion and R-loop suppression. Cell Rep.
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WB, IB, PCA
(). BCG administration promotes the long-term protection afforded by a single-dose intranasal adenovirus-based SARS-CoV-2 vaccine. iScience.
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WB, IB, PCA
(). Methylmalonyl-Coa Mutase and 2-Oxoglutarate Dehydrogenase Interact with Each Other on the Inner Mitochondrial Membrane. SSRN
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WB, IB, PCA
(). Dominant-Negative Form of SIGIRR: SIGIRRΔE8 Promotes Tumor Growth Through Regulation of Metabolic Pathways. J Interferon Cytokine Res.
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IP, Co-IP; WB, IB, PCA
(). First-in-human autologous implantation of genetically modified adipocytes expressing LCAT for the treatment of familial LCAT deficiency. Heliyon.
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WB, IB, PCA
(). Transcriptome-wide identification of RNA-binding protein binding sites using seCLIP-seq. Nat Protoc.
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WB, IB, PCA
(). S6K1 phosphorylates Cdk1 and MSH6 to regulate DNA repair. Elife.
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WB, IB, PCA
(). Crosstalk between pro-survival sphingolipid metabolism and complement signaling induces inflammasome-mediated tumor metastasis. Cell Rep.
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WB, IB, PCA
(). Anti‑inflammatory effects of Nypa fruticans Wurmb via NF‑κB and MAPK signaling pathways in macrophages. Exp Ther Med.
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WB, IB, PCA
(). Identification and functional characterization of transcriptional activators in human cells. Mol Cel.
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WB, IB, PCA
(). A FBXO7/EYA2-SCFFBXW7 axis promotes AXL-mediated maintenance of mesenchymal and immune evasion phenotypes of cancer cells. Mol Cell.
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WB, IB, PCA
(). Listeria toxin promotes phosphorylation of the inflammasome adaptor ASC through Lyn and Syk to exacerbate pathogen expansion. Cell Rep.
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WB, IB, PCA
(). CTNNBL1 restricts HIV-1 replication by suppressing viral DNA integration into the cell genome. Cell Rep.
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WB, IB, PCA
(). The PAF1 complex promotes 3' processing of pervasive transcripts. Cell Rep.
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WB, IB, PCA
(). Therapeutic modulation of GSTO activity rescues FUS-associated neurotoxicity via deglutathionylation in ALS disease models. Dev Cell.
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WB, IB, PCA
(). Natterin-Induced Neutrophilia Is Dependent on cGAS/STING Activation via Type I IFN Signaling Pathway. Lima C et al.
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WB, IB, PCA
(). Active APPL1 sequestration by Plasmodium favors liver-stage development. Cell Rep.
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WB, IB, PCA
(). A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency. Cell Rep.
Applications
IP, Co-IP
(). Inhibition of Karyopherin β1-Mediated Nuclear Import Disrupts Oncogenic Lineage-Defining Transcription Factor Activity in Small Cell Lung Cancer. Cancer Res.
Applications
WB, IB, PCA
(). The membrane-associated ubiquitin ligases MARCH2 and MARCH3 target IL-5 receptor alpha to negatively regulate eosinophilic airway inflammation. Cell Mol Immunol.
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WB, IB, PCA
(). Regulation of membrane fluidity by RNF145‐triggered degradation of the lipid hydrolase ADIPOR2. EMBO J.
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WB, IB, PCA
(). Induction of natural IgE by glucocorticoids. J Exp Med.
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WB, IB, PCA
(). TET3 epigenetically controls feeding and stress response behaviors via AGRP neurons. J Clin Invest.
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WB, IB, PCA
(). Cytidine analogs are synthetic lethal with base excision repair default due to MBD4 deficiency. NPJ Precis. Oncol.
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WB, IB, PCA
(). Reciprocal effects of mTOR inhibitors on pro-survival proteins dictate therapeutic responses in tuberous sclerosis complex. iScience.
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IP, Co-IP; WB, IB, PCA
(). An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity. Nucleic Acids Res.
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IP, Co-IP
(). Scalable and deep profiling of mRNA targets for individual microRNAs with chimeric eCLIP. bioRxiv Preprint
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WB, IB, PCA
(). STAT and Janus kinase targeting by human herpesvirus 8 interferon regulatory factor in the suppression of type-I interferon signaling. PloS Pathogens
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WB, IB, PCA
(). Single-nucleus RNA-sequencing in pre-cellularization Drosophila melanogaster embryos. PloS One
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WB, IB, PCA
(). High glutamine suppresses osteogenesis through mTORC1-mediated inhibition of the mTORC2/AKT-473/RUNX2 axis. Cell Death Discovery
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WB, IB, PCA; IP, Co-IP
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WB, IB, PCA
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IP, Co-IP
(). ATase inhibition rescues age-associated proteotoxicity of the secretory pathway. Communications Biology
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WB, IB, PCA; IP, Co-IP
(). Nitrate transport via NRT2.1 mediates NIN-LIKE PROTEIN-dependent suppression of root nodulation in Lotus japonicus. The Plant Cell
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WB, IB, PCA
(). Regulation of immune receptor kinase plasma membrane nanoscale organization by a plant peptide hormone and its receptors. ELife
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WB, IB, PCA
(). The ubiquitin-dependent ATPase p97 removes cytotoxic trapped PARP1 from chromatin. Nat Cell Biol.
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WB, IB, PCA
(). MYCN recruits the nuclear exosome complex to RNA polymerase II to prevent transcription-replication conflicts. Mol Cell.
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WB, IB, PCA
(). C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stress. Cells.
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WB, IB, PCA
(). RIPK1 Promotes Energy Sensing by the mTORC1 Pathway. Mol Cell
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WB, IB, PCA
(). Choroid plexus NKCC1 mediates cerebrospinal fluid clearance during mouse early postnatal development. Nat Commun
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WB, IB, PCA
(). Identification of potential regulatory domains within the MreC and MreD components of the cell elongation machinery. J Bacteriol
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Undefined
(). Kidney injury molecule-1 is a potential receptor for SARS-CoV-2. J Mol Cell Biol
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WB, IB, PCA
(). Angiocrine IGFBP3 spatially coordinates IGF signaling during neonatal cardiac regeneration. bioRxiv Preprint
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(). Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms. Cell Rep.
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(). A targetable LIFR− NF-κB− LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis. Nat Commun.
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WB, IB, PCA
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Applications
Undefined
(). Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes. Cell.
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WB, IB, PCA
(). RLTPR Q575E: A novel recurrent gain‐of‐function mutation in patients with adult T‐cell leukemia/lymphoma. Eur J Haematol.
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WB, IB, PCA
(). NRF2 promotes urothelial cell response to bacterial infection by regulating reactive oxygen species and RAB27B expression. Cell Rep.
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IP, Co-IP
(). Alanine Catabolism of Paneth Cells Maintains Intestinal Stem Cell Function During Dietary Restriction. Cell Press Preprint
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WB, IB, PCA
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WB, IB, PCA
(). S-acylation is a positive regulator of FLS2-mediated plant immunity. bioRxiv Preprint
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WB, IB, PCA
(). USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2. Theranostics.
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WB, IB, PCA
(). Endoplasmic reticulum acetyltransferases Atase1 and Atase2 differentially regulate reticulophagy, macroautophagy and cellular acetyl-CoA metabolism. Commun Biol.
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WB, IB, PCA
(). Theta-burst stimulation of primary afferents drives long-term potentiation in the spinal cord and persistent pain via α2δ-1–bound NMDA receptors. J Neurosci.
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WB, IB, PCA
(). VELCRO-IP RNA-seq reveals ribosome expansion segment function in translation genome-wide. Cell Rep.
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WB, IB, PCA
(). Phase separation of OCT4 controls TAD reorganization to promote cell fate transitions. Cell Stem Cell.
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WB, IB, PCA
(). Cnksr2 loss in mice leads to increased neural activity and behavioral phenotypes of Epilepsy-Aphasia Syndrome J Neurosci.
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WB, IB, PCA
(). Effects of temperature on proliferation of myoblasts from donor piglets with different thermoregulatory maturities. BMC Mol Cell Biol.
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WB, IB, PCA
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WB, IB, PCA
(). Protective effects of Camellia sinensis fruit and fruit peels against oxidative DNA damage. KoreaScience
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WB, IB, PCA
(). Anti-Inflammatory Effects of Abeliophyllum distichum Nakai (Cultivar Okhwang 1) Callus through Inhibition of PI3K/Akt, NF-κB, and MAPK Signaling Pathways in Lipopolysaccharide-Induced Macrophages. Processes.
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WB, IB, PCA
(). Super-resolution microscopy reveals that Na+/K+-ATPase signaling protects against glucose-induced apoptosis by deactivating Bad. Cell Death Dis.
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WB, IB, PCA
(). Single-nucleus RNA-sequencing in pre-cellularization Drosophila melanogaster embryos. bioRxiv Preprint
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WB, IB, PCA
(). Subunit-dependent and independent rules of AMPA receptor trafficking during long-term depression in hippocampal neurons. J Biol Chem.
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WB, IB, PCA
(). Characterisation of a novel KRAS G12C inhibitor ASP2453 that shows potent anti-tumour activity in KRAS G12C-mutated preclinical models. Br J Cancer.
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WB, IB, PCA
(). CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progression. Autophagy.
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WB, IB, PCA
(). Phosphorylation of Collapsin Response Mediator Protein 1 (CRMP1) at Tyrosine 504 residue regulates Semaphorin 3A‐induced cortical dendritic growth. J Neurochem.
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WB, IB, PCA
(). α2δ-1 Upregulation in primary sensory neurons promotes NMDA receptor-mediated glutamatergic input in resiniferatoxin-induced neuropathy. J Neurosci.
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WB, IB, PCA
(). E2F4's cytoplasmic role in multiciliogenesis is mediated via an N-terminal domain that binds two components of the centriole replication machinery, Deup1 and SAS6. Mol Biol Cell.
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WB, IB, PCA
(). Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains. Mol Cell.
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WB, IB, PCA
(). Interplay of Trypanosome Lytic Factor and innate immune cells in the resolution of cutaneous Leishmania infection. PLoS Pathog.
Applications
WB, IB, PCA
(). TRF2-mediated ORC recruitment underlies telomere stability upon DNA replication stress. Nucleic Acids Res.
Applications
WB, IB, PCA
(). fSHAPE, fSHAPE-eCLIP, and SHAPE-eCLIP probe transcript regions that interact with specific proteins. STAR Protoc.
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WB, IB, PCA
(). The PAF1 Complex Recruits Integrator to Chromatin Globally to Terminate Non-Productive Transcription. Cell Press Preprint
Applications
WB, IB, PCA
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Applications
WB, IB, PCA
(). Inhibition of Vif-Mediated Degradation of APOBEC3G through Competitive Binding of Core-Binding Factor Beta. J Virol
Applications
WB, IB, PCA
(). PIKES analysis reveals response to degraders and key regulatory mechanisms of the CRL4 network. Mol Cell
Applications
WB, IB, PCA
(). Calcineurin inhibition causes α2δ-1–mediated tonic activation of synaptic NMDA receptors and pain hypersensitivity J Neurosci
Applications
IP, Co-IP
(). SUGT1 controls susceptibility to HIV-1 infection by stabilizing microtubule plus-ends. Cell Death Diff
Applications
WB, IB, PCA
(). Nuclear Accumulation of LAP1: TRF2 Complex during DNA Damage Response Uncovers a Novel Role for LAP1. Cells
Applications
WB, IB, PCA
(). A Non-canonical PDK1-RSK Signal Diminishes Pro-caspase-8-Mediated Necroptosis Blockade. Mol Cell .
Applications
IP, Co-IP; WB, IB, PCA
(). A Family of Argonaute-Interacting Proteins Gates Nuclear RNAi. Mol Cell
Applications
WB, IB, PCA
(). A phospho-switch controls RNF43-mediated degradation of Wnt receptors to suppress tumorigenesis. Nat Commun
Applications
WB, IB, PCA
(). Kelch-like protein 42 is a pro-fibrotic ubiquitin E3 ligase involved in in systemic sclerosis. Journal of Biological Chemistry
Applications
IP, Co-IP; WB, IB, PCA
(). Juvenile OLFM4 Null Mice Are Protected from Sepsis. American Journal of Physiology
Applications
Other
(). Protective and inhibitory effects of acteoside from Abeliophyllum distichum Nakai against oxidative DNA damage. Molecular Medicine Reports
Applications
WB, IB, PCA
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Applications
IP, Co-IP
(). Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription. Cell Stem Cell.
Applications
Undefined
(). Identification of a Small-Molecule Inhibitor That Disrupts the SIX1/EYA2 Complex, EMT, and Metastasis. Cancer Res.
Applications
IP, Co-IP
(). Identification of a Small-Molecule Inhibitor That Disrupts the SIX1/EYA2 Complex, EMT, and Metastasis. Cancer Res.
Applications
WB, IB, PCA
(). KAP1 Is a Chromatin Reader that Couples Steps of RNA Polymerase II Transcription to Sustain Oncogenic Programs. Mol Cell.
Applications
IP, Co-IP
(). Protective and inhibitory effects of acteoside from Abeliophyllum distichum Nakai against oxidative DNA damage. Mol Med Rep.
Applications
WB, IB, PCA
(). eIF2B as a Target for Viral Evasion of PKR-Mediated Translation Inhibition. mBio.
Applications
WB, IB, PCA
(). Distinct IL-1α-responsive enhancers promote acute and coordinated changes in chromatin topology in a hierarchical manner. EMBO J.
Applications
WB, IB, PCA
(). All You Ever Wanted to Know About APOL1 and TLFs and Did Not Dare Ask. Methods Mol Biol.
Applications
WB, IB, PCA
(). Targeting Opsin4/Melanopsin with a Novel Small Molecule Suppresses PKC/RAF/MEK/ERK Signaling and Inhibits Lung Adenocarcinoma Progression. Mol Cancer Res.
Applications
WB, IB, PCA
(). Depletion of Vasohibin 1 Speeds Contraction and Relaxation in Failing Human Cardiomyocytes. Circ Res.
Applications
WB, IB, PCA
(). Modulation of virus-induced NF-κB signaling by NEMO coiled coil mimics. Nat Commun.
Applications
IP, Co-IP
(). Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors. Nat Methods.
Applications
WB, IB, PCA
(). Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer. Cell Death Differ.
Applications
WB, IB, PCA
(). An Essential Membrane Protein Modulates the Proteolysis of LpxC to Control Lipopolysaccharide Synthesis in Escherichia coli. mBio.
Applications
WB, IB, PCA
(). Transcriptome-wide profiles of circular RNA and RNA binding protein interactions reveal effects on circular RNA biogenesis and cancer pathway expression. Genome Med .
Applications
WB, IB, PCA
(). FERONIA regulates FLS2 plasma membrane nanoscale dynamics to modulate plant immune signaling. bioRxiv
Applications
WB, IB, PCA
(). A Comprehensive Map of the Monocyte-Derived Dendritic Cell Transcriptional Network Engaged upon Innate Sensing of HIV. Cell Rep.
Applications
WB, IB, PCA
(). YAP/TAZ direct commitment and maturation of lymph node fibroblastic reticular cells. Nat Commun.
Applications
WB, IB, PCA
(). Irisin: Still chasing shadows. Mol Metab.
Applications
WB, IB, PCA
(). REV7 is required for processing AID initiated DNA lesions in activated B cells. Nat Commun.
Applications
WB, IB, PCA
(). Interplay of the Trypanosome Lytic Factor and innate immune cells in the resolution of cutaneous Leishmania infection. bioRxiv
Applications
WB, IB, PCA
(). Consequences of Maternal Essential Fatty Acid and Conjugated Linoleic Acid Supplementation on the Development of Calf Muscle and Adipose Tissue. Animals : An Open Access Journal from Mdpi
Applications
WB, IB, PCA
(). Longitudinal RNA-Seq Analysis of the Repeatability of Gene Expression and Splicing in Human Platelets Identifies a Platelet SELP Splice QTL. Circulation Research
Applications
WB, IB, PCA
(). SIRT3, a metabolic target linked to ataxia-telangiectasia mutated (ATM) gene deficiency in diffuse large B-cell lymphoma. Sci Rep.
Applications
IP, Co-IP
(). Fbxo7 and Pink1 play a reciprocal role in regulating their protein levels. Aging (Albany NY).
Applications
WB, IB, PCA
(). Oxidative Stress Culminates in Intracellular Bacteria Expulsion Via NRF2/KEAP1 Induction of RAB27B. Cell Press Preprint
Applications
WB, IB, PCA
(). Ruxolitinib attenuates intimal hyperplasia via inhibiting JAK2/STAT3 signaling pathway activation induced by PDGF-BB in vascular smooth muscle cells. Microvasc Res.
Applications
WB, IB, PCA
(). WSTF acetylation by MOF promotes WSTF activities and oncogenic functions. Oncogene.
Applications
WB, IB, PCA
(). Dynamic regulation of mitochondrial import by the ubiquitin system. Mol Cell.
Applications
WB, IB, PCA
(). Gene-and Species-Specific hox mRNA translation by ribosome expansion segments. Mol Cell.
Applications
WB, IB, PCA
(). Energy sensor AMPK gamma regulates translation via phosphatase PPP6C independent of AMPK alpha. Mol Cell.
Applications
IP, Co-IP
(). Bone-Specific Overexpression of PITX1 Induces Senile Osteoporosis in Mice Through Deficient Self-Renewal of Mesenchymal Progenitors and Wnt Pathway Inhibition. Sci Rep.
Applications
WB, IB, PCA
(). SGK phosphorylates Cdc25 and Myt1 to trigger cyclin B–Cdk1 activation at the meiotic G2/M transition J Cell Biol
Applications
WB, IB, PCA
(). Inducible Natural IgMs Bridge Trypanosome Lytic Factor Assembly and Parasite Recognition. Cell Host Microbe
Applications
Undefined
(). The Crohn's Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy. Molecular Cell
Applications
WB, IB, PCA
(). The Crohn's Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy. Molecular Cell
Applications
IP, Co-IP
(). Broad-spectrum receptor tyrosine kinase inhibitors overcome de novo and acquired modes of resistance to EGFR-targeted therapies in colorectal cancer. Oncotarget
Applications
WB, IB, PCA
(). Differential transactivation of the upstream aggrecan enhancer regulated by PAX1/9 depends on SOX9-driven transactivation. Scientific Reports
Applications
IP, Co-IP; WB, IB, PCA
(). Microbial recognition by GEF-H1 controls IKKε mediated activation of IRF5. Nature Communications
Applications
IP, Co-IP
(). Apurinic/apyrimidinic endonuclease 1 (APE1) is dispensable for activation-induced cytidine deaminase (AID)-dependent somatic hypermutation in the immunoglobulin gene. Int Immunol.
Applications
WB, IB, PCA
(). The Hippo Pathway Blocks Mammalian Retinal Müller Glial Cell Reprogramming. Cell Rep.
Applications
IP, Co-IP
(). BIN1 favors the spreading of Tau via extracellular vesicles. Sci Rep.
Applications
WB, IB, PCA
(). Unique and Shared Epigenetic Programs of the CREBBP and EP300 Acetyltransferases in Germinal Center B Cells Reveal Targetable Dependencies in Lymphoma. Immunity.
Applications
WB, IB, PCA
(). YAP/TAZ Activation Drives Uveal Melanoma Initiation and Progression. Cell Rep.
Applications
WB, IB, PCA
(). p53-Pirh2 Complex Promotes Twist1 Degradation and Inhibits EMT. Mol Cancer Res.
Applications
WB, IB, PCA
(). Arginine methylation of FOXP3 is crucial for the suppressive function of regulatory T cells. J Autoimmun.
Applications
WB, IB, PCA
(). Hippo kinase NDR2 inhibits IL-17 signaling by promoting Smurf1-mediated MEKK2 ubiquitination and degradation. Mol Immunol.
Applications
WB, IB, PCA
(). Z-ring membrane anchors associate with cell wall synthases to initiate bacterial cell division. Nat Commun.
Applications
WB, IB, PCA
(). Protein kinase Cα-mediated phosphorylation of Twist1 at Ser-144 prevents Twist1 ubiquitination and stabilizes it. J Biol Chem.
Applications
WB, IB, PCA
(). Phosphorylation of Syntaxin 17 by TBK1 Controls Autophagy Initiation. Dev Cell.
Applications
Undefined
(). Methods to Characterize Protein Interactions with β-Arrestin In Cellulo. Methods Mol Biol.
Applications
WB, IB, PCA
(). SMPDL3b modulates insulin receptor signaling in diabetic kidney disease. Nat Commun.
Applications
WB, IB, PCA
(). TAM Kinases Promote Necroptosis by Regulating Oligomerization of MLKL. Mol Cell.
Applications
WB, IB, PCA
(). Maternal pluripotency factors initiate extensive chromatin remodelling to predefine first response to inductive signals. Nat Commun.
Applications
WB, IB, PCA; IP, Co-IP
(). Glucocorticoids and myosin5b loss of function induce heightened PKA signaling in addition to membrane traffic defects. Mol Biol Cell.
Applications
WB, IB, PCA
(). Irradiated Human Fibroblasts as a Substitute Feeder Layer to Irradiated Mouse 3T3 for the Culture of Human Corneal Epithelial Cells: Impact on the Stability of the Transcription Factors Sp1 and NFI. Int J Mol Sci.
Applications
WB, IB, PCA
(). Missense variants in NOX1 and p22phox in a case of very-early-onset inflammatory bowel disease are functionally linked to NOD2. Cold Spring Harb Mol Case Stud.
Applications
WB, IB, PCA
(). Pink1 regulates FKBP5 interaction with AKT/PHLPP and protects neurons from neurotoxin stress induced by MPP. J Neurochem.
Applications
WB, IB, PCA
(). HIV-1 Envelope Overcomes NLRP3-Mediated Inhibition of F-Actin Polymerization for Viral Entry. Cell Rep.
Applications
WB, IB, PCA
(). Prdm9 and meiotic cohesin proteins cooperatively promote DNA double-strand break formation in mammalian spermatocytes. Curr Biol.
Applications
ISH, FISH
(). PRDM14 directly interacts with heat shock proteins HSP90α and glucose-regulated protein 78. Cancer Science
Applications
IP, Co-IP; WB, IB, PCA
(). β-Secretase BACE1 Promotes Surface Expression and Function of Kv3.4 at Hippocampal Mossy Fiber Synapses. The Journal of Neuroscience
Applications
IP, Co-IP
(). Development of an efficient chromatin immunoprecipitation method to investigate protein-DNA interaction in oleaginous castor bean seeds. PLOS One
Applications
WB, IB, PCA
(). DHX9 regulates production of hepatitis B virus-derived circular RNA and viral protein levels. Oncotarget
Applications
WB, IB, PCA
(). IL1RAP potentiates multiple oncogenic signaling pathways in AML. Journal of Experimental Medicine
Applications
IP, Co-IP
(). Phosphocode-dependent functional dichotomy of a common co-receptor in plant signalling. Nature
Applications
WB, IB, PCA
(). The long non-coding RNA NEAT1 is elevated in polyglutamine repeat expansion diseases and protects from disease gene-dependent toxicities. Human Molecular Genetics
Applications
IP, Co-IP; WB, IB, PCA
(). The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. Elife
Applications
IP, Co-IP
(). Mechanism of Stx17 recruitment to autophagosomes via IRGM and mammalian Atg8 proteins. Journal of Cell Biology
Applications
IP, Co-IP; WB, IB, PCA
(). Inhibition of MicroRNA-155 Supports Endothelial Tight Junction Integrity Following Oxygen-Glucose Deprivation. Journal of the American Heart Association
Applications
WB, IB, PCA
(). Gankyrin Promotes Tumor-Suppressor Protein Degradation to Drive Hepatocyte Proliferation. Cellular and Molecular Gastroenterology and Hepatology
Applications
WB, IB, PCA
(). Epsin15 Homology Domains: Role in the Pathogenesis of Pulmonary Arterial Hypertension. Frontiers in Physiology
Applications
WB, IB, PCA
(). Z-ring membrane anchors associate with cell wall synthases to initiate bacterial cell division. Nature Communications
Applications
WB, IB, PCA
(). Sestrin2 facilitates glutamine-dependent transcription of PGC-1α and survival of liver cancer cells under glucose limitation. FEBS J.
Applications
WB, IB, PCA; IP, Co-IP
(). Modulation of Protein-Interaction States through the Cell Cycle. Cell.
Applications
IP, Co-IP
(). PELI1 Selectively Targets Kinase-Active RIP3 for Ubiquitylation-Dependent Proteasomal Degradation. Mol Cell.
Applications
WB, IB, PCA; IP, Co-IP
(). Effect of dietary palmitic and stearic acids on sucrose motivation and hypothalamic and striatal cell signals in the rat. Am J Physiol Regul Integr Comp Physiol.
Applications
WB, IB, PCA
(). A functional interplay between Δ133p53 and ΔNp63 in promoting glycolytic metabolism to fuel cancer cell proliferation. Oncogene.
Applications
IP, Co-IP
(). Tumor necrosis factor-α-induced protein 8-like-2 is involved in the activation of macrophages by Astragalus polysaccharides in vitro. Mol Med Rep.
Applications
WB, IB, PCA
(). The interaction between IKKα and LC3 promotes type I interferon production through the TLR9-containing LAPosome. Sci Signal.
Applications
WB, IB, PCA
(). NMDA receptor-dependent dephosphorylation of serine 387 in Argonaute 2 increases its degradation and affects dendritic spine density and maturation. J Biol Chem.
Applications
WB, IB, PCA
(). The Autism-Related Protein CHD8 Cooperates with C/EBPβ to Regulate Adipogenesis. Cell Rep.
Applications
WB, IB, PCA
(). Apelin and APJ orchestrate complex tissue-specific control of cardiomyocyte hypertrophy and contractility in the hypertrophy-heart failure transition. Am J Physiol Heart Circ Physiol.
Applications
WB, IB, PCA
(). Transcriptome-pathology correlation identifies interplay between TDP-43 and the expression of its kinase CK1E in sporadic ALS. Acta Neuropathol.
Applications
WB, IB, PCA
(). Protein kinase A activation inhibits DUX4 gene expression in myotubes from patients with facioscapulohumeral muscular dystrophy. J Biol Chem.
Applications
WB, IB, PCA
(). Dermatan sulfate epimerase 1 and dermatan 4-O-sulfotransferase 1 form complexes that generate long epimerized 4-O-sulfated blocks. J Biol Chem.
Applications
WB, IB, PCA
(). Active N6-Methyladenine Demethylation by DMAD Regulates Gene Expression by Coordinating with Polycomb Protein in Neurons. Mol Cell.
Applications
WB, IB, PCA
(). Somatic and Germline MicroRNAs Form Distinct Silencing Complexes to Regulate Their Target mRNAs Differently. Dev Cell.
Applications
IP, Co-IP; WB, IB, PCA
(). The role of maternal pioneer factors in predefining first zygotic responses to inductive signals. bioRxiv
Applications
WB, IB, PCA
(). Flap endonuclease 1 is involved in cccDNA formation in the hepatitis B virus. PLoS Pathog.
Applications
WB, IB, PCA
(). The SMILE transcriptional corepressor inhibits cAMP response element-binding protein (CREB)-mediated transactivation of gluconeogenic genes. J Biol Chem.
Applications
WB, IB, PCA
(). Expression of Human Cytomegalovirus IE1 Leads to Accumulation of Mono-SUMOylated PML That Is Protected from Degradation by Herpes Simplex Virus 1 ICP0. J Virol.
Applications
WB, IB, PCA
(). NONO Detects the Nuclear HIV Capsid to Promote cGAS-Mediated Innate Immune Activation. Cell. 2018;175(2):488-501.e22. doi:10.1016/j.cell.2018.08.062 Cell.
Applications
WB, IB, PCA
(). Protein Phosphotyrosine Phosphatase 1B (PTP1B) in Calpain-dependent Feedback Regulation of Vascular Endothelial Growth Factor Receptor (VEGFR2) in Endothelial Cells: IMPLICATIONS IN VEGF-DEPENDENT ANGIOGENESIS AND DIABETIC WOUND HEALING. Journal of Biological Chemistry
Applications
IP, Co-IP
(). A Cytoplasmic RNA Virus Alters the Function of the Cell Splicing Protein SRSF2. Journal of Virology
Applications
IP, Co-IP
(). Interference of the complex between NCS-1 and Ric8a with phenothiazines regulates synaptic function and is an approach for fragile X syndrome. Proc. Natl. Acad. Sci. U.S.A
Applications
IP, Co-IP; WB, IB, PCA
(). Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6. Scientific Reports
Applications
WB, IB, PCA
(). Vitreous Humor Changes Expression of Iron-Handling Proteins in Lens Epithelial Cells. Investigative Ophthalmology & Visual Science
Applications
WB, IB, PCA
(). Novel small molecule inhibiting CDCP1-PKCδ pathway reduces tumor metastasis and proliferation. Cancer Science
Applications
IP, Co-IP; WB, IB, PCA
(). NLRP1 promotes tumor growth by enhancing inflammasome activation and suppressing apoptosis in metastatic melanoma. Oncogene
Applications
IP, Co-IP; WB, IB, PCA
(). Gene and protein expression of a soluble form of CTLA-4 in a healthy dog. Journal of Veterinary Medical Science
Applications
IP, Co-IP; WB, IB, PCA
(). An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice. Cell Stem Cell
Applications
IF, Confocal Microscopy; WB, IB, PCA
(). DOK7 gene therapy enhances motor activity and life span in ALS model mice. EMBO Molecular Medicine
Applications
IP, Co-IP; WB, IB, PCA
(). RNA Binding Protein CUGBP1 Inhibits Liver Cancer in a Phosphorylation-Dependent Manner. Molecular and Cellular Biology
Applications
WB, IB, PCA
(). Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy. Current Biology
Applications
IP, Co-IP
(). Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions. Methods
Applications
WB, IB, PCA
(). POPX2 phosphatase regulates apoptosis through the TAK1-IKK-NF-κB pathway. Cell Death & Disease
Applications
WB, IB, PCA
(). The Cdc45/RecJ-like protein forms a complex with GINS and MCM, and is important for DNA replication in Thermococcus kodakarensis. Nucleic Acids Research
Applications
WB, IB, PCA
(). A TNF-p100 pathway subverts noncanonical NF-κB signaling in inflamed secondary lymphoid organs. The EMBO Journal
Applications
IP, Co-IP; WB, IB, PCA
(). p120-Catenin is an obligate haploinsufficient tumor suppressor in intestinal neoplasia. Journal of Clinical Investigation
Applications
WB, IB, PCA
(). Mechanistic Insights into Autoinhibition of the Oncogenic Chromatin Remodeler ALC1. Molecular Cell
Applications
IP, Co-IP
(). Cell cycle-dependent phosphorylation regulates RECQL4 pathway choice and ubiquitination in DNA double-strand break repair. Nature Communications
Applications
IP, Co-IP; WB, IB, PCA
(). Zinc finger protein ZPR9 functions as an activator of AMPK-related serine/threonine kinase MPK38/MELK involved in ASK1/TGF-β/p53 signaling pathways. Scientific Reports
Applications
IP, Co-IP; WB, IB, PCA
(). The Legionella effector WipB is a translocated Ser/Thr phosphatase that targets the host lysosomal nutrient sensing machinery. Scientific Reports
Applications
WB, IB, PCA
(). Possible function of the second RecJ-like protein in stalled replication fork repair by interacting with Hef. Scientific Reports
Applications
WB, IB, PCA
(). PrimPol is required for replication reinitiation after mtDNA damage. Proc Natl Acad Sci U S A.
Applications
WB, IB, PCA
(). SFPQ associates to LSD1 and regulates the migration of newborn pyramidal neurons in the developing cerebral cortex. Int J Dev Neurosci.
Applications
WB, IB, PCA
(). The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Cell.
Applications
WB, IB, PCA
(). Analyses of a Mutant Foxp3 Allele Reveal BATF as a Critical Transcription Factor in the Differentiation and Accumulation of Tissue Regulatory T Cells. Immunity.
Applications
WB, IB, PCA
(). Human cytomegalovirus-encoded viral cyclin-dependent kinase (v-CDK) UL97 phosphorylates and inactivates the retinoblastoma protein-related p107 and p130 proteins. J Biol Chem.
Applications
WB, IB, PCA
(). Increased expression of thyroid hormone responsive protein (THRSP) is the result but not the cause of higher intramuscular fat content in cattle. Int J Biol Sci.
Applications
WB, IB, PCA
(). Phosphorylation of transcriptional regulators in the retinoblastoma protein pathway by UL97, the viral cyclin-dependent kinase encoded by human cytomegalovirus. Virology.
Applications
WB, IB, PCA
(). A Short cis-Acting Motif in the M112-113 Promoter Region Is Essential for IE3 To Activate M112-113 Gene Expression and Is Important for Murine Cytomegalovirus Replication. J Virol.
Applications
WB, IB, PCA
(). Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis? Scientific Reports
Applications
WB, IB, PCA
(). Skin Keratins. Methods in Enzymology
Applications
IP, Co-IP
(). Dissecting the Roles of Polycomb Repressive Complex 2 Subunits in the Control of Skin Development. Journal of Investigative Dermatology
Applications
WB, IB, PCA
(). Oral administration of Aloe vera gel powder prevents UVB-induced decrease in skin elasticity via suppression of overexpression of MMPs in hairless mice. Bioscience, Biotechnology, and Biochemistry
Applications
WB, IB, PCA
(). Molecular Basis of the Interaction of the Human Protein Tyrosine Phosphatase Non-receptor Type 4 (PTPN4) with the Mitogen-activated Protein Kinase p38γ. Journal of Biological Chemistry
Applications
WB, IB, PCA
(). RNA Exosome Complex Regulates Stability of the Hepatitis B Virus X-mRNA Transcript in a Non-stop-mediated (NSD) RNA Quality Control Mechanism. Journal of Biological Chemistry
Applications
WB, IB, PCA
(). Thioredoxin reductase 1 suppresses adipocyte differentiation and insulin responsiveness. Scientific Reports
Applications
IP, Co-IP
(). Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes. Journal of Biological Chemistry
Applications
IP, Co-IP; WB, IB, PCA
(). BubR1 alterations that reinforce mitotic surveillance act against aneuploidy and cancer. Elife
Applications
IP, Co-IP; WB, IB, PCA
(). Satellite RNAs promote pancreatic oncogenic processes via the dysfunction of YBX1. Nature Communications
Applications
WB, IB, PCA
(). Opposing roles for SNAP23 in secretion in exocrine and endocrine pancreatic cells. Journal of Cell Biology
Applications
IP, Co-IP
(). Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a. Nature Cell Biology
Applications
IP, Co-IP
(). Basigin can be a therapeutic target to restore the retinal vascular barrier function in the mouse model of diabetic retinopathy. Scientific Reports
Applications
IP, Co-IP
(). The chromatin modifying complex CoREST/LSD1 negatively regulates notch pathway during cerebral cortex development. Dev Neurobiol.
Applications
IP, Co-IP
(). Activation of the thrombopoietin receptor by mutant calreticulin in CALR-mutant myeloproliferative neoplasms. Blood.
Applications
WB, IB, PCA
(). Pitchfork and Gprasp2 Target Smoothened to the Primary Cilium for Hedgehog Pathway Activation. PLoS One.
Applications
IP, Co-IP
(). TRIP13 promotes error-prone nonhomologous end joining and induces chemoresistance in head and neck cancer. Nat Commun.
Applications
WB, IB, PCA
(). The miR-17∼92 cluster contributes to MLL leukemia through the repression of MEIS1 competitor PKNOX1. Leuk Res.
Applications
WB, IB, PCA
(). Functional cooperation between KCa3.1 and TRPC1 channels in human breast cancer: Role in cell proliferation and patient prognosis. Oncotarget.
Applications
IP, Co-IP
(). Loss of the antioxidant enzyme CuZnSOD (Sod1) mimics an age-related increase in absolute mitochondrial DNA copy number in the skeletal muscle. Age (Dordr).
Applications
WB, IB, PCA
(). Soluble ADAM33 initiates airway remodeling to promote susceptibility for allergic asthma in early life. JCI Insight.
Applications
WB, IB, PCA
(). Sequential actions of SIRT1-RELB-SIRT3 coordinate nuclear-mitochondrial communication during immunometabolic adaptation to acute inflammation and sepsis. Journal of Biological Chemistry
Applications
IP, Co-IP; WB, IB, PCA
(). Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes. Journal of Cell Biology
Applications
IP, Co-IP
(). MEI4 – a central player in the regulation of meiotic DNA double-strand break formation in the mouse. Journal of Cell Science
Applications
IP, Co-IP; WB, IB, PCA
(). Maturation of the proteasome core particle induces an affinity switch that controls regulatory particle association. Nature Communications
Applications
WB, IB, PCA
(). Lipopolysaccharide Induces Alveolar Macrophage Necrosis via CD14 and the P2X7 Receptor Leading to Interleukin-1α Release. Immunity
Applications
IP, Co-IP
(). Eosinophil-specific deletion of IκBα in mice reveals a critical role of NF-κB-induced Bcl-xL for inhibition of apoptosis. Blood
Applications
WB, IB, PCA
(). TRAF1 Coordinates Polyubiquitin Signaling to Enhance Epstein-Barr Virus LMP1-Mediated Growth and Survival Pathway Activation. PLOS Pathogens
Applications
WB, IB, PCA
(). Hypertension-causing Mutations in Cullin3 Protein Impair RhoA Protein Ubiquitination and Augment the Association with Substrate Adaptors. Journal of Biological Chemistry
Applications
IP, Co-IP; WB, IB, PCA
(). BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins. American Journal of Physiology-Cell Physiology
Applications
WB, IB, PCA
(). Differential Effects of Hepatocyte Nuclear Factor 4α Isoforms on Tumor Growth and T-Cell Factor 4/AP-1 Interactions in Human Colorectal Cancer Cells. Molecular and Cellular Biology
Applications
ChIP; WB, IB, PCA
(). A caveolin-dependent and PI3K/AKT-independent role of PTEN in β-catenin transcriptional activity. Nature Communications
Applications
IP, Co-IP; WB, IB, PCA
(). Shedding of Endogenous Interleukin-6 Receptor (IL-6R) Is Governed by A Disintegrin and Metalloproteinase (ADAM) Proteases while a Full-length IL-6R Isoform Localizes to Circulating Microvesicles. Journal of Biological Chemistry
Applications
WB, IB, PCA
(). Generation of aminoterminally truncated, stable types of bioactive bovine and porcine fibroblast growth factor 4 in Escherichia coli. Biotechnol Appl Biochem.
Applications
IP, Co-IP
(). HSPB8 and the Cochaperone BAG3 Are Highly Expressed During the Synthetic Phase of Rat Myometrium Programming During Pregnancy. Biol Reprod.
Applications
WB, IB, PCA
(). Chronic FLT3-ITD Signaling in Acute Myeloid Leukemia Is Connected to a Specific Chromatin Signature. Cell Rep.
Applications
WB, IB, PCA
(). Quantitative and dynamic analysis of PTEN phosphorylation by NMR. Methods.
Applications
WB, IB, PCA
(). Localization and abundance of early markers of fat cell differentiation in the skeletal muscle of cattle during growth--are DLK1-positive cells the origin of marbling flecks? Meat Sci.
Applications
WB, IB, PCA
(). TGF-β suppression of HBV RNA through AID-dependent recruitment of an RNA exosome complex. PLoS Pathog.
Applications
WB, IB, PCA
(). Silencing of PMEPA1 accelerates the growth of prostate cancer cells through AR, NEDD4 and PTEN. Oncotarget.
Applications
WB, IB, PCA
(). Molecular Determinants for the Inactivation of the Retinoblastoma Tumor Suppressor by the Viral Cyclin-dependent Kinase UL97. J Biol Chem.
Applications
WB, IB, PCA
(). Role of extracellular matrix in development of skeletal muscle and postmortem aging of meat. Meat Sci.
Applications
WB, IB, PCA
(). G0/G1 Switch Gene 2 Regulates Cardiac Lipolysis. J Biol Chem.
Applications
WB, IB, PCA
(). Siglec1 suppresses antiviral innate immune response by inducing TBK1 degradation via the ubiquitin ligase TRIM27. Cell Res.
Applications
WB, IB, PCA
(). Differential regulation of S-region hypermutation and class switch recombination by noncanonical functions of uracil DNA glycosylase. Proc Natl Acad Sci USA
Applications
ChIP; IP, Co-IP
(). A Parkinson's disease gene regulatory network identifies the signaling protein RGS2 as a modulator of LRRK2 activity and neuronal toxicity. Hum Mol Genet.
Applications
WB, IB, PCA
(). Activation of the MCM helicase from the thermophilic archaeon, Thermoplasma acidophilum by interactions with GINS and Cdc6-2. Extremophiles.
Applications
WB, IB, PCA
(). Matrix metalloproteinase-9 is involved in chronic lymphocytic leukemia cell response to fludarabine and arsenic trioxide. PLoS One.
Applications
WB, IB, PCA
(). The nuclear form of glutathione peroxidase 4 colocalizes and directly interacts with protamines in the nuclear matrix during mouse sperm chromatin assembly. Spermatogenesis.
Applications
WB, IB, PCA
(). Locus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle? PLoS One.
Applications
WB, IB, PCA
(). Toll-like receptor 4 and MAIR-II/CLM-4/LMIR2 immunoreceptor regulate VLA-4-mediated inflammatory monocyte migration. Nat Commun.
Applications
WB, IB, PCA
(). OFD1 and flotillins are integral components of a ciliary signaling protein complex organized by polycystins in renal epithelia and odontoblasts. PLoS One.
Applications
WB, IB, PCA
(). Distinct patterns of phosphatidylserine localization within the Rab11a-containing recycling system. Cell Logist.
Applications
WB, IB, PCA
(). Impact of dietary protein level and source of polyunsaturated fatty acids on lipid metabolism-related protein expression and fatty acid concentrations in porcine tissues. J Agric Food Chem.
Applications
WB, IB, PCA
(). Lipoprotein lipase (LPL) is associated with neurite pathology and its levels are markedly reduced in the dentate gyrus of Alzheimer's disease brains. The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Applications
WB, IB, PCA
(). Rab11-family interacting proteins define spatially and temporally distinct regions within the dynamic Rab11a-dependent recycling system. Mol Biol Cell.
Applications
WB, IB, PCA
(). RAB8B is required for activity and caveolar endocytosis of LRP6. Cell Rep.
Applications
WB, IB, PCA
(). Early adipogenesis is regulated through USP7-mediated deubiquitination of the histone acetyltransferase TIP60. Nat Commun.
Applications
IP, Co-IP
(). High-content analysis of proapoptotic EphA4 dependence receptor functions using small-molecule libraries. J Biolmol Screen
Applications
WB, IB, PCA
(). Leucine zipper domain is required for Kaposi sarcoma-associated herpesvirus (KSHV) K-bZIP protein to interact with histone deacetylase and is important for KSHV replication. J Biol Chem.
Applications
WB, IB, PCA
(). Agouti revisited: transcript quantification of the ASIP gene in bovine tissues related to protein expression and localization. PLoS One.
Applications
WB, IB, PCA
(). Conversion of vascular endothelial cells into multipotent stem-like cells. Nat Med.
Applications
IP, Co-IP
(). PLTP regulates STAT3 and NFκB in differentiated THP1 cells and human monocyte-derived macrophages. Biochim Biophys Acta.
Applications
WB, IB, PCA
(). H2B homology region of major immediate-early protein 1 is essential for murine cytomegalovirus to disrupt nuclear domain 10, but is not important for viral replication in cell culture. J Gen Virol.
Applications
WB, IB, PCA
(). c-Jun N-terminal kinase phosphorylates DCP1a to control formation of P bodies. J Cell Biol.
Applications
WB, IB, PCA
(). Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation. Nucleic Acids Res.
Applications
WB, IB, PCA
(). Molecular characterization of SMILE as a novel corepressor of nuclear receptors. Nucleic Acids Res.
Applications
WB, IB, PCA
(). Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation. J Biol Chem.
Applications
WB, IB, PCA; IP, Co-IP
(). Tautomycetin suppresses the TNFα/NF-κB pathway via inhibition of IKK activation. Int J Oncol
Applications
IP, Co-IP
(). c-Jun controls histone modifications, NF-kappaB recruitment, and RNA polymerase II function to activate the ccl2 gene. Mol Cell Biol. 2008;28(13):4407-4423. doi:10.1128/MCB.00535-07 Mol Cell Biol.
Applications
WB, IB, PCA
(). Isoform-specific defects of insulin stimulation of Akt/protein kinase B (PKB) in skeletal muscle cells from type 2 diabetic patients. Diabetologia.
Applications
WB, IB, PCA
(). A feedback loop between the androgen receptor and a NEDD4-binding protein, PMEPA1, in prostate cancer cells. J Biol Chem.
Applications
WB, IB, PCA
(). Beta-arrestin 2 is required for lysophosphatidic acid-induced NF-kappaB activation. Proc Natl Acad Sci U S A.
Applications
WB, IB, PCA
(). Selective cochlear degeneration in mice lacking the F-box protein, Fbx2, a glycoprotein-specific ubiquitin ligase subunit. J Neurosci.
Applications
WB, IB, PCA
(). PRR5, a novel component of mTOR complex 2, regulates platelet-derived growth factor receptor beta expression and signaling. J Biol Chem.
Applications
IP, Co-IP
(). Interaction of hemojuvelin with neogenin results in iron accumulation in human embryonic kidney 293 cells. J Biol Chem.
Applications
WB, IB, PCA

What is Rockland’s TrueBlot® product line?

Rockland’s TrueBlot® product line is designed to solve common experimental problems when performing immunoprecipitation/co-immunoprecipitation experiments and Western blots of immunoprecipitated samples (IP–Western blot). The product line consists of TrueBlot® monoclonal secondary antibodies and TrueBlot® IP beads.

What is the advantage of TrueBlot® secondaries over regular secondary antibodies?

TrueBlot® antibodies are specific to the whole IgG molecule and will not bind heavy or light chains. This is useful for binding target proteins with an expected MW near 25 kDa (light chains) and 50 kDa (heavy chains).

Why does it appear that the TrueBlot® antibody is binding heavy/light chains in my sample?

The sample must be fully reduced to eliminate cross-reactivity with heavy and light chains. Any reactivity to heavy and light chains could be due to incomplete reduction of your sample. Please be sure to optimize reducing conditions for your sample type.

How can I ensure my samples are fully reduced?

Samples can be fully reduced by heating at 90-100°C for 10 minutes in sample buffer containing reducing agent (for example, DTT to a final concentration of 50 mM or add β-Mercaptoethanol to a final concentration of 2% (v/v)).

With which species/isotypes are TrueBlot® secondary antibodies reactive?

TrueBlot® secondary antibodies are reactive with the IgG of their respective species. For example, if using a primary antibody raised in mouse (mouse host), use TrueBlot® anti-mouse Ig secondary antibodies for detection.

Do TrueBlot® secondary antibodies detect IgM?

TrueBlot® secondary antibodies have not been shown to detect IgM.

Do I need to preclear my lysate before the immunoprecipitation step?

Samples that have many other proteins present (such as lysates) may require preclearing to prevent interference in later IP and WB procedures. Recombinant protein samples require pre-clearing more often than serum samples.

How can I enrich/concentrate my lysate for a low expression of a protein of interest?

The immunoprecipitation procedure can be repeated several times to yield a more concentrated protein solution.

What is the recommended lysis buffer for TrueBlot® products?

The optimal lysis buffer will depend on your sample type. See protocol for buffer recommendations. Generally, 1X RIPA is used to lyse samples.

My target protein has the same MW as a heavy/light chain. How can I be sure that the band is the target protein and not a heavy/light chain?

Be sure to include a positive control for the primary antibody in your experiment and check that the sample is fully reduced.

What is the average size of TrueBlot® magnetic/agarose beads?

The beads are roughly 0.5 μm in diameter.

Why can’t I use Protein A or Protein G beads for the IP step in IP–Western blot when using rabbit species?

Using Protein A or Protein G beads with rabbit species results in contaminated bands. For best results when using rabbit species, use Rockland’s TrueBlot® Rabbit Ig IP beads, which are available in either a magnetic or agarose format. Please see individual TrueBlot® Rabbit product pages for additional details.

Can I use Protein A or Protein G beads for the IP step in IP–Western blot when using mouse, goat, or sheep species?

Yes. If you are using Protein A or Protein G beads with mouse, goat, or sheep species, we recommend the following: Determine the compatibility of Protein A or Protein G with your species and subtype, do not use excessive amounts of slurry, and choose the appropriate elution conditions. In some instances, harsher elution conditions can cause stripping of the subunits of Protein A or Protein G from the bead support and result in non-specific bands. For best results, we recommend using TrueBlot® Ig IP beads, which are available in either agarose or magnetic format.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.