Rabbit IgG F(c) Antibody DyLight™ 680 Conjugated
Anti-Tubulin and Anti-TNFa Antibodies - Western Blot
Western Blot of Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n 611-1103)
DyLight™ 680 Fluorescence Spectra.
Properties of DyLight™ Conjugates.
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Datasheet

Rabbit IgG F(c) Antibody DyLight™ 680 Conjugated

Goat Polyclonal

611-144-003
100 µg
WB
Goat
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Description

Background

Anti-Rabbit IgG F(c) DyLight generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

Application Note

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum.  No reaction was observed against Rabbit IgG F(ab).  This antibody will react with heavy chains of Rabbit IgG.  Minimal reactivity is expected against other Rabbit immunoglobulins.

Anti-RABBIT IgG F(c) (GOAT) Antibody DyLight™ 680 Conjugated - 611-144-003
Goat Anti Rabbit IgG F(c) DyLight 680™ Conjugated Antibody, Goat Anti-Rabbit IgG Fc Fragment Antibody DyLight 680™ conjugation, Goat Anti Rabbit IgG Fc Antibody DyLight 680™ conjugated
WB
Rabbit
Goat
DyLight™ 680
Polyclonal
IgG
Rabbit IgG F(c) fragment
2.8 moles DL680 per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Raimo S et al. Mitochondrial morphology, bioenergetics and proteomic responses in fatty acid oxidation disorders. Redox Biol. (2021)
Applications
WB, IB, PCA
Meese et al. Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin. Frontiers in Synaptic Neuroscience (2017)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.