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Background

Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. To enable detection, the secondary antibody must have specificity for the antibody species and isotype of the primary antibody being used and generally is conjugated. Rockland produces highly active antibodies and conjugates against mouse immunoglobulins. Anti-Mouse Secondary Antibodies are affinity-purified polyclonal antibodies with well-characterized specificity for mouse immunoglobulin classes, subclasses, and fragments. They are available as unlabeled antibodies as well as conjugates of alkaline phosphatase (AP), horseradish peroxidase (HRP), fluorescent conjugates, and biotin. This Anti-MOUSE IgG generated in rabbit is designed to detect heavy and light chains (H&L) and has been Peroxidase (HRP) conjugated. Rigorous quality control testing ensures that the finished product meets or exceeds out high standards for optimum performance in your assays. These secondary antibodies are used throughout various types of assays, including ELISA or Western Blot, Immunohistochemistry, Flow Cytometry. Optimal secondary antibody requires knowledge of the detection assay.

Product Details

Rabbit Anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated - 610-4302
Rabbit Anti-Mouse IgG Secondary Antibody Peroxidase Conjugated, Rabbit Anti-Mouse IgG Secondary Antibody HRP Conjugated, RAM-HRP, Anti-mouse secondary antibody, anti-mouse HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-mouse horseradish peroxidase conjugated secondary antibody
Rabbit
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG

Target Details

Mouse
Mouse IgG whole molecule
HRP Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.

Application Details

ELISA, WB
IF, Other  - View References
Anti-Mouse secondary antibody conjugated to horseradish peroxidase (HRP) generated in rabbit detects specifically Mouse IgG whole molecule. Anti-Mouse IgG peroxidase antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation

2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store mouse secondary antibody conjugate at 4° C prior to restoration.   For extended storage aliquot antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Mitochondrial H2O2 release does not directly cause damage to chromosomal DNA. Nat Commun.
Applications
WB, IB, PCA
(). Effectiveness of VSV vectored SARS-CoV-2 spike when administered through intranasal, intramuscular or a combination of both. Sci Rep.
Applications
WB, IB, PCA
(). Ferritin microheterogeneity, subunit composition, functional, and physiological implications. Sci Rep.
Applications
WB, IB, PCA
(). Immunity to Seasonal Coronavirus Spike Proteins Does Not Protect from SARS-CoV-2 Challenge in a Mouse Model but Has No Detrimental Effect on Protection Mediated by COVID-19 mRNA Vaccination. J Virol.
Applications
EIA, ELISA
(). Extracellular vesicles from gastric epithelial GES-1 cells infected with Helicobacter pylori promote changes in recipient cells associated with malignancy. Front Oncol.
Applications
WB, IB, PCA
(). Palmitoyl‑RGD promotes the expression of dermal‑epidermal junction components in HaCaT cells. Mol Med Rep.
Applications
WB, IB, PCA
(). The plasmablast response to SARS-CoV-2 mRNA vaccination is dominated by non-neutralizing antibodies that target both the NTD and the RBD. medRxiv.
Applications
E, EIA
(). Introduction of Two Prolines and Removal of the Polybasic Cleavage Site Lead to Higher Efficacy of a Recombinant Spike-Based SARS-CoV-2 Vaccine in the Mouse Model. mBio.
Applications
E, EIA
(). SARS-CoV-2 mRNA vaccination induces functionally diverse antibodies to NTD, RBD, and S2. Cell.
Applications
EIA, ELISA
(). Vaccination with SARS-CoV-2 variants of concern protects mice from challenge with wild-type virus. PLoS One.
Applications
EIA, ELISA
(). Endolysosomal Targeting of Mitochondria Is Integral to BAX-Mediated Mitochondrial Permeabilization during Apoptosis Signaling. Dev Cell.
Applications
WB, IB, PCA
(). An in vitro microneutralization assay for SARS‐CoV‐2 serology and drug screening. Curr Protoc Microbiol.
Applications
E, EIA; Other
(). Introduction of two prolines and removal of the polybasic cleavage site leads to optimal efficacy of a recombinant spike based SARS-CoV-2 vaccine in the mouse model. ASM
Applications
E, EIA
(). Regulation of Mitochondrial Respiratory Chain Complex Levels, Organization, and Function by Arginyltransferase 1. Front Cell Dev Biol
Applications
WB, IB, PCA
(). Transcutaneous codelivery of tumor antigen and resiquimod in solid-in-oil nanodispersions promotes antitumor immunity. ACS Biomater Sci Eng.
Applications
Undefined
(). Utility of Hybrid Transferrin Binding Protein Antigens for Protection Against Pathogenic Neisseria Species. Frontiers in Immunology
Applications
EIA, ELISA
(). The influenza virus hemagglutinin head evolves faster than the stalk domain. Scientific Reports
Applications
IF, Confocal Microscopy
(). Obesity, diabetes, and leptin resistance promote tau pathology in a mouse model of disease. Neuroscience
Applications
WB, IB, PCA
(). Postmortem Pittsburgh Compound B (PiB) binding increases with Alzheimer's disease progression. Journal of Alzheimer's Disease : Jad
Applications
WB, IB, PCA
(). Characterization of the human folate receptor alpha via novel antibody-based probes. Oncotarget
Applications
EIA, ELISA
(). Localization and induction of the A2 virulence factor in Leishmania: evidence that A2 is a stress response protein. Mol Microbiol.
Applications
WB, IB, PCA
(). Effects of nonsteroidal anti-inflammatory drugs on amyloid-beta pathology in mouse skeletal muscle. Neurobiology of Disease
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.