Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated
Dylight conjugated antibody - Western Blot
DyLight™ 800 Fluorescence Spectra.
Properties of DyLight™ Fluorescent Dyes.
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Datasheet

Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated

Rabbit Polyclonal

610-445-002 610-445-002-0.5
100 µg 5 x 100 µg
WB
Mouse
Rabbit
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$95.00 /Per Item
$162.00 /Per Item
100 µg $95.00
5 x 100 µg $162.00
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Description

Background

Anti-Mouse IgG DyLight 800 Antibody generated in rabbit detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Note

Anti-Mouse IgG DyLight 800 Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Conjugated Anti-Mouse IgG is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. DyLight conjugated antibodies are effective alternatives to Alexa 800 and IR Dye 800 conjugates. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Purity/Specificity

This Dye Conjugated Second Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. This antibody will react with heavy chains of Mouse IgG and with light chains of most Mouse immunoglobulins.

Anti-MOUSE IgG (H&L) (RABBIT) Antibody DyLight™ 800 Conjugated - 610-445-002
rabbit anti-Mouse IgG Antibody DyLight™ 800 conjugation, rabbit anti-Mouse IgG DyLight™800 conjugated Antibody
WB
Mouse
Mouse
Rabbit
DyLight™ 800
Polyclonal
IgG
Mouse IgG, whole molecule
2.5 moles DL800 per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Balic JJ et al. STAT3 serine phosphorylation is required for TLR4 metabolic reprogramming and IL-1β expression. Nat Commun. (2020)
Applications
WB, IB, PCA
Mitra S, Bodor DL, David AF, et al. Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin. Nat Commun. (2020)
Applications
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Wang H et al. A simple and highly purified method for isolation of glomeruli from the mouse kidney. Am J Physiol Renal Physiol. (2019)
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Zhang Y et al. Non-genomic mechanisms mediate androgen-induced PSD95 expression. Aging (Albany NY). (2019)
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Zhang et al. Amelioratory Effects of Testosterone Propionate on Age-related Renal Fibrosis via Suppression of TGF-β1/Smad Signaling and Activation of Nrf2-ARE Signaling. Scientific Reports (2018)
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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.