Western and Immunofluorescence using Mouse IgG

Datasheet SDS

Mouse IgG

Name: Mouse IgG
Brand: Rockland Immunochemicals
Availability: InStock

13 References

Item No. 010-0102

Size: 25 mg

Applications: ELISA, IF, FC, SDS-PAGE, Multiplex, Other

Origin: Mouse

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25 mg $0.00

1 g $0.00

5 mg $220.00

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Background

Mouse IgG purified protein (Immunoglobulin G) are antibody molecules. Mouse IgG is composed of four peptide chains — two heavy chains and two light chains. Mouse IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. Mouse IgG typically constitutes 75% of serum immunoglobulins. Mouse IgG molecules are synthesized and secreted by plasma B cells. Ideal as a negative control for Flow Cytometry, Western blotting, immunoprecipitation and immunohistochemistry applications.

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Specifications for Mouse IgG

Product Details

Description: Mouse IgG Whole Molecule (BULK ORDER) - 010-0102

Synonyms: Immunoglobulin G from Mouse, IgG from Mouse, purified polyclonal IgG antibody

Species of Origin: Mouse

Format: IgG

Type: Native Protein

Target Details

Purity/Specificity: Mouse IgG was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.

Application Details

Tested Applications: SDS-PAGE

Suggested Applications: ELISA, FC, IF, Other, Multiplex - View References

Application Note: Mouse IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Formulation

Physical State: Lyophilized

Concentration: 10.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Reconstitution Volume: 2.5 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store purified Mouse IgG at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (13)

Neumeier, D et al. (2022). Phenotypic determinism and stochasticity in antibody repertoires of clonally expanded plasma cells. Proceedings of the National Academy of Sciences of the United States of America

Applications

FC, FACS, FLOW

PubMed

Arakaki AKS et al. (2021). α-arrestin ARRDC3 tumor suppressor function is linked to GPCR-induced TAZ activation and breast cancer metastasis. J Cell Sci.

Applications

Undefined

PubMed

Neumeier D et al. (2021). Phenotypic determinism and stochasticity in antibody repertoires of clonally expanded plasma cells. bioRxiv Preprint

Applications

EIA, ELISA

Source

Mason BM et al. (2021). Optimization of therapeutic antibodies by predicting antigen specificity from antibody sequence via deep learning. Nat Biomed Eng.

Applications

Undefined

PubMed

Fair-Makela R et al. (2020). Robo4 contributes to the turnover of Peyer's patch B cells. Mucosal Immunol.

Applications

Other

PubMed

Matsumura T et al. (2019). Sequential Sensing by TLR2 and Mincle Directs Immature Myeloid Cells to Protect against Invasive Group A Streptococcal Infection in Mice. Cell Rep

Applications

Control

PubMed

Zhang Z et al. (2019). Elav-mediated exon skipping and alternative polyadenylation of the Dscam1 gene are required for axon outgrowth. Cell Rep

Applications

qRT-PCR, RT-PCR

PubMed

Kawakami Y et al. (2018). The soluble form of LOTUS inhibits Nogo receptor-mediated signaling by interfering with the interaction between Nogo receptor type 1 and p75 neurotrophin receptor. J Neurosci.

Applications

E, EIA; Other

PubMed

Kruse N et al. (2016). A First Tetraplex Assay for the Simultaneous Quantification of Total α-Synuclein, Tau, β-Amyloid42 and DJ-1 in Human Cerebrospinal Fluid. PloS One

Applications

E, EIA

PubMed

Bronietzki M et al. (2014). Study of phagolysosome biogenesis in live macrophages. J Vis Exp.

Applications

Ab Immobilization

PubMed

Li, D et al. (2013). Targeting the EphB4 receptor for cancer diagnosis and therapy monitoring. Molecular Pharmaceutics

Applications

WB, IB, PCA; IF, Confocal Microscopy; Immunization

PubMed

Penaranda, C et al. (2011). Anti-CD3 therapy promotes tolerance by selectively depleting pathogenic cells while preserving regulatory T cells. Journal of Immunology (Baltimore, Md. : 1950)

Applications

FC, FACS, FLOW; Cellular assay

PubMed

Ono K et al. (2006). Caenorhabditis elegans kettin, a large immunoglobulin-like repeat protein, binds to filamentous actin and provides mechanical stability to the contractile apparatuses in body wall muscle. Mol Biol Cell.

Applications

IF, Confocal Microscopy; Multiplex Assay

PubMed

Related Protocols

ELISA Protocol

Flow Cytometry (FC) Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

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Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.