Mouse IgG
Mouse IgG Whole Molecule SDS-Page
SDS-PAGE of Mouse IgG Whole Molecule
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Datasheet

Mouse IgG

010-0102 010-0102-1000 010-0102-0005
25 mg 1 g 5 mg
SDS-PAGE, ELISA, IF, Other
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$197.00 /Per Item
25 mg $0.00
1 g $0.00
5 mg $197.00
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Description

Background

Mouse IgG purified protein (Immunoglobulin G) are antibody molecules. Mouse IgG is composed of four peptide chains — two heavy chains and two light chains. Mouse IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. Mouse IgG typically constitutes 75% of serum immunoglobulins. Mouse IgG molecules are synthesized and secreted by plasma B cells. Ideal as a negative control for Flow Cytometry, Western blotting, immunoprecipitation and immunohistochemistry applications.

Application Note

Mouse IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Purity/Specificity

Mouse IgG was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum.

MOUSE IgG whole molecule (BULK ORDER) - 010-0102
Immunoglobulin G from Mouse, IgG from Mouse, purified polyclonal IgG antibody
SDS-PAGE
ELISA, IF, Other
Mouse
IgG
Protein
Native Protein
Lyophilized
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
2.5 mL
Restore with deionized water (or equivalent)
Ambient
Store purified Mouse IgG at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Arakaki AKS et al. α-arrestin ARRDC3 tumor suppressor function is linked to GPCR-induced TAZ activation and breast cancer metastasis. J Cell Sci. (2021)
Applications
Undefined
Neumeier D et al. Phenotypic determinism and stochasticity in antibody repertoires of clonally expanded plasma cells. bioRxiv Preprint (2021)
Applications
EIA, ELISA
Mason BM et al. Optimization of therapeutic antibodies by predicting antigen specificity from antibody sequence via deep learning. Nat Biomed Eng. (2021)
Applications
Undefined
Fair-Makela R et al. Robo4 contributes to the turnover of Peyer's patch B cells. Mucosal Immunol. (2020)
Applications
Other
Matsumura T et al. Sequential Sensing by TLR2 and Mincle Directs Immature Myeloid Cells to Protect against Invasive Group A Streptococcal Infection in Mice. Cell Rep (2019)
Applications
Undefined
Zhang Z et al. Elav-mediated exon skipping and alternative polyadenylation of the Dscam1 gene are required for axon outgrowth. Cell Rep (2019)
Applications
qRT-PCR, RT-PCR
Kawakami Y et al. The soluble form of LOTUS inhibits Nogo receptor-mediated signaling by interfering with the interaction between Nogo receptor type 1 and p75 neurotrophin receptor. J Neurosci. (2018)
Applications
E, EIA; Other
Kruse N et al. A First Tetraplex Assay for the Simultaneous Quantification of Total α-Synuclein, Tau, β-Amyloid42 and DJ-1 in Human Cerebrospinal Fluid. PloS One (2016)
Applications
E, EIA
Bronietzki M et al. Study of phagolysosome biogenesis in live macrophages. J Vis Exp. (2014)
Applications
Ab Immobilization
Ono K et al. Caenorhabditis elegans kettin, a large immunoglobulin-like repeat protein, binds to filamentous actin and provides mechanical stability to the contractile apparatuses in body wall muscle. Mol Biol Cell. (2006)
Applications
IF, Confocal Microscopy

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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