THRA Antibody
Anti-THRA Antibody - Western Blot
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Datasheet

THRA Antibody

Rabbit Polyclonal

600-401-A38S 600-401-A38
25 µL 100 µg
ELISA, WB, ChIP, IHC
Human
Rabbit
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$99.00 /Per Item
$399.00 /Per Item
25 µL $99.00
100 µg $399.00
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Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Thyroid hormone receptor alpha is a nuclear hormone receptor with high affinity for the hormone triiodo-thyronine.  THRA is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having a certain extent of redundancy, may mediate different functions of thyroid hormone.  THRA interacts with NCOA3 and NCOA6 co-activators, leading to a strong increase in transcription of target genes. THRA is localized within the nucleus and has been found to exist as 4 isoforms originating from alternative splicing variants.  This antibody recognizes THRA isoform 1.  Isoform 1 has a distinct C-terminus compared to isoform 2.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting.

Purity/Specificity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human THRA protein.  A BLAST analysis was used to suggest cross-reactivity with THRA from mouse, human and rat based on a 100% homology with the immunizing sequence.  Cross-reactivity with THRA from other sources has not been determined.

Anti-Thyroid Hormone Receptor alpha (THRA) (RABBIT) Antibody - 600-401-A38
rabbit anti-THRA antibody, rabbit anti-Thyroid hormone receptor alpha antibody, Nuclear receptor subfamily 1 group A member 1, c-erbA-alpha, c-erbA-1, V-erbA-related protein 7, EAR7, ERBA1, NR1A1, THRA1, THRA2
THRA
ELISA, WB
ChIP, IHC
Human
Human
Rabbit
Polyclonal
IgG
Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human THRA isoform 1 protein.
Liquid (sterile filtered)
1.0mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Gao X et al. Thyroid hormone receptor beta and NCOA4 regulate terminal erythrocyte differentiation. Proc Natl Acad Sci USA. (2017)
Applications
WB, IB, PCA
Contreras-Jurado C et al. Impaired hair growth and wound healing in mice lacking thyroid hormone receptors. PloS One (2014)
Applications
IHC, ICC, Histology
Mishra A et al. Adipogenesis is differentially impaired by thyroid hormone receptor mutant isoforms. J Mol Endocrinol. (2010)
Applications
ChIP

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.