RNF34 Antibody
Anti-RNF34 Antibody - Western Blot
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Datasheet

RNF34 Antibody

Rabbit Polyclonal

200-401-933
500 µg
ELISA, WB
Human
Rabbit
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$456.00 /Per Item
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Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). RNF34, also known as RING finger protein 34, RING finger protein RIFF, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, hRFI, Caspases-8 and -10-associated RING finger protein 1, CARP-1 and Caspase regulator CARP1, is a novel modulator of NF-kB activation.  RNF34 possesses E3 ubiquitin protein ligase activity and has been shown to directly and indirectly regulate the levels of p53/TP53 through ubiquitination, targeting to proteasomal degradation and degradation of SFN. Anti-RNF34 Antibody is useful for researcher interested in Immunology, p53 regulation, Transcription, and Nuclear Signaling research.

Application Note

This protein A purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 42 kDa in size corresponding to RNF34 protein by western blotting in the appropriate tissue or cell lysate or extract.  Isoforms 1 and 2, which are equivalent in size, should both cross-react with this antibody.

Purity/Specificity

This protein A purified antibody is directed against human RNF34 protein.  The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.  A BLAST analysis was used to suggest cross-reactivity with RNF34 protein from orangutan (99%), bovine (91%), dog (89%), mouse (88%) and rat (85%) based on a high degree of protein:protein homology with the immunizing sequence.  Expect reactivity with isoform 1 and 2 of RNF34.  Reactivity against homologues from other sources is not known.

Anti-RNF34 (RABBIT) Antibody - 200-401-933
rabbit anti-RNF34 Antibody, rabbit anti-RNF 34 Antibody, Caspase regulator CARP1 antibody, CARP 1 antibody, hRFI antibody, RFI antibody, RIFF antibody, Ring finger protein 34 antibody
RNF34
ELISA, WB
Human
Human
Rabbit
Polyclonal
IgG
Recombinant Protein
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-373 of human RNF34 protein.
Lyophilized
5.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
500 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.