Ready-To-Use RFP Antibody Pre-adsorbed

Background

Fluorescent proteins like green fluorescent protein (GFP) and Discosoma Red Fluorescent Protein (DsRFP) are widely used in research. DsRFP is derived from the mushroom anemone Discosoma sp. Despite sharing only 19% similarity, anti-GFP antibodies typically don't recognize DsRFP and vice versa. Structurally, DsRFP is akin to GFP in its overall β-can fold and chromophore-formation chemistry. However, GFP undergoes an extra step in chromophore maturation and maintains a tetrameric structure. By using site-directed mutagenesis, various DsRFP variants have been engineered, enabling the red fluorescent protein to mature as a monomer. These monomeric DsRFP variants—mRFP1, mBanana, mCherry, mHoneydew, mPlum, mOrange, mStrawberry, and mTangerine—provide a diverse range of fluorescent colors. Rockland's RFP polyclonal antibodies, raised against the entire wild-type RFP protein, are expected to recognize all these variant forms. These antibodies have been pre-absorbed to prevent potential cross-reactivity with human, mouse, and rat serum proteins, and have also been confirmed to not react with GFP protein. All Rockland Immunochemical's RFP antibodies undergo stringent affinity purification to ensure high specificity and affinity. Thorough quality control tests guarantee that the final product meets or exceeds the highest standards, ensuring optimal performance in your assays.