RFP Antibody
Anti-RFP Antibody - Western Blot
Western Blot of Chicken Anti-RFP Antibody
Immunocytochemistry of Chicken Anti-RFP
Immunocytochemistry of Chicken Anti-RFP
Immunocytochemistry of Chicken Anti-RFP
Immunohistochemistry of Chicken Anti-RFP
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Datasheet

RFP Antibody

Chicken Polyclonal

600-901-379S 600-901-379
25 µL 100 µg
ELISA, SDS-PAGE, WB, FC, IF, IHC
RFP, tdTomato
Chicken
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$99.00 /Per Item
$240.00 /Per Item
25 µL $99.00
100 µg $240.00
Availability: Ships next business day Availability: Ships next business day
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays such as western blotting.

Application Note

Anti-RFP is designed to detect recombinant RFP. Anti-RFP antibody has been tested by ELISA, SDS-PAGE, and western blot to detect RFP. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.

Purity/Specificity

RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above.  RFP Antibody was tested by western blot.

Anti-RFP (CHICKEN) Antibody - 600-901-379
chicken anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
DsRed
ELISA, SDS-PAGE, WB
FC, IF, IHC
RFP, tdTomato
RFP
Chicken
Polyclonal
IgY
Recombinant Protein
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Xie Y et al. Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse development. Cell Rep. (2022)
Applications
IF, Confocal Microscopy
Roth E et al. Behavioural and neurochemical mechanisms underpinning the feeding-suppressive effect of GLP-1/CCK combinatorial therapy. Mol Metab. (2021)
Applications
IF, Confocal Microscopy
Wu H et al. Distinct subtypes of proprioceptive dorsal root ganglion neurons regulate adaptive proprioception in mice. Nature Communications. (2021)
Applications
FC, FACS, FLOW; IF, Confocal Microscopy
Hara A et al. Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity. Genes Cells. (2021)
Applications
IF, Confocal Microscopy
Campbell LA et al. Protein-retention expansion microscopy for visualizing subcellular organelles in fixed brain tissue. J Neurosci Methods. (2021)
Applications
IHC, ICC, Histology
Nestor-Kalinoski A et al. Unique neural circuit connectivity of mouse proximal, middle, and distal colon defines regional colonic motor patterns. Cell Mol Gastroenterol Hepatol. (2021)
Applications
IHC, ICC, Histology
Garcia MT et al. Transcriptional profiling of sequentially generated septal neuron fates. Elife. (2021)
Applications
IF, Confocal Microscopy
Baldwin KT et al. HepaCAM controls astrocyte self-organization and coupling. Neuron. (2021)
Applications
Undefined
Chipman PH et al. Astrocyte GluN2C NMDA receptors control basal synaptic strengths of hippocampal CA1 pyramidal neurons in the stratum radiatum. Elife. (2021)
Applications
IF, Confocal Microscopy; IHC, ICC, Histology
Trebels, B., Dippel, S., Schaaf, M. et al. Adult neurogenesis in the mushroom bodies of red flour beetles (Tribolium castaneum, HERBST) is influenced by the olfactory environment. Sci Rep (2020)
Applications
IF, Confocal Microscopy
Chen PY et al. Smooth muscle cell reprogramming in aortic aneurysms. Cell Stem Cell. (2020)
Applications
FC, FACS, FLOW; IF, Confocal Microscopy
Goodson NB et al. Simultaneous deletion of Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs from deleting both genes. Development. (2020)
Applications
IHC, ICC, Histology
Goodson NB et al. Prdm1 overexpression causes a photoreceptor fate-shift in nascent, but not mature, bipolar cells. Dev Biol. (2020)
Applications
Undefined
DeSisto J et al. Single-cell transcriptomic analyses of the developing meninges reveal meningeal fibroblast diversity and function. Dev Cell. (2020)
Applications
Undefined
Shibata-Germanos S, Goodman JR, Grieg A, et al. Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges. Acta Neuropathol. (2020)
Applications
IHC, ICC, Histology
Di Matteo F, Pipicelli F, Kyrousi C, et al. Cystatin B is essential for proliferation and interneuron migration in individuals with EPM1 epilepsy. EMBO Mol Med. (2020)
Applications
IHC, ICC, Histology
Padmashri R, Ren B, Oldham B, Jung Y, Gough R, Dunaevsky A. Modeling Human-specific Interlaminar Astrocytes in the Mouse Cerebral Cortex. J Comp Neurol. (2020)
Applications
IHC, ICC, Histology
Drake KA, Fessler AR, Carroll TJ. Methods for renal lineage tracing: In vivo and beyond. Methods Cell Biol. (2019)
Applications
Undefined
Pignatelli M, Ryan TJ, Roy DS, et al. Engram Cell Excitability State Determines the Efficacy of Memory Retrieval. Neuron. (2019)
Applications
IHC, ICC, Histology
Korrell KV, Disser J, Parley K, et al. Differential effect on myelination through abolition of activity-dependent synaptic vesicle release or reduction of overall electrical activity of selected cortical projections in the mouse. J Anat. (2019)
Applications
IHC, ICC, Histology
Kim D, An H, Shearer RS, et al. A principled strategy for mapping enhancers to genes. Sci Rep. (2019)
Applications
IF, Confocal Microscopy
Ko KI, Syverson AL, Kralik RM, et al. Diabetes-Induced NF-κB Dysregulation in Skeletal Stem Cells Prevents Resolution of Inflammation. Diabetes. (2019)
Applications
IF, Confocal Microscopy
Delventhal R, O'Connor RM, Pantalia MM, et al. Dissection of central clock function in Drosophila through cell-specific CRISPR-mediated clock gene disruption. Elife. (2019)
Applications
IHC, ICC, Histology
Wang Y et al. A cell fitness selection model for neuronal survival during development. Nat Commun. (2019)
Applications
IHC, ICC, Histology
Aleyasin et al. Cell-Type-Specific Role of ΔFosB in Nucleus Accumbens In Modulating Intermale Aggression. The Journal of Neuroscience (2018)
Applications
IF, Confocal Microscopy
Kelley et al. Variation among intact tissue samples reveals the core transcriptional features of human CNS cell classes. Nature Neuroscience (2018)
Applications
IHC, ICC, Histology
Heude et al. Unique morphogenetic signatures define mammalian neck muscles and associated connective tissues. Elife (2018)
Applications
IF, Confocal Microscopy
Orlandi C, Omori Y, Wang Y, et al. Transsynaptic Binding of Orphan Receptor GPR179 to Dystroglycan-Pikachurin Complex Is Essential for the Synaptic Organization of Photoreceptors. Cell Rep. (2018)
Applications
WB, IB, PCA
Zacharias WJ, Frank DB, Zepp JA, et al. Regeneration of the lung alveolus by an evolutionarily conserved epithelial progenitor. Nature. (2018)
Applications
IHC, ICC, Histology
Che A, Babij R, Iannone AF, et al. Layer I Interneurons Sharpen Sensory Maps during Neonatal Development. Neuron. (2018)
Applications
IF, Confocal Microscopy
van Lessen et al. Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development. Elife (2017)
Applications
IF, Confocal Microscopy
Lechner et al. Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy. Cell Stem Cell (2017)
Applications
IF, Confocal Microscopy
Paul et al. Transcriptional Architecture of Synaptic Communication Delineates GABAergic Neuron Identity. Cell (2017)
Applications
IF, Confocal Microscopy
Ghitani N, Barik A, Szczot M, et al. Specialized Mechanosensory Nociceptors Mediating Rapid Responses to Hair Pull. Neuron. (2017)
Applications
IF, Confocal Microscopy
Ding et al. Schlafen 4-expressing myeloid-derived suppressor cells are induced during murine gastric metaplasia. Journal of Clinical Investigation (2016)
Applications
WB, IB, PCA
Kockel et al. A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research. G3: Genes, Genomes, Genetics (2016)
Applications
IHC, ICC, Histology
Kivelä et al. The transcription factor Prox1 is essential for satellite cell differentiation and muscle fibre-type regulation. Nature Communications (2016)
Applications
IHC, ICC, Histology
Haller et al. Ubiquitination and proteasomal degradation of ATG12 regulates its proapoptotic activity. Autophagy (2015)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.