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Datasheet Protocol SDS

Collagen Type I Antibody

Rabbit Polyclonal

226 References
600-401-103S 600-401-103-0.1 600-401-103-0.5
25 µL 100 µg 500 µg
Liquid (sterile filtered) Liquid (sterile filtered) Liquid (sterile filtered)
WB, ELISA, IHC, IF, FC, Dot Blot, IP, Multiplex
Human, Mouse, Rat, Bovine, Pig
Rabbit
$125.00 /Per Item
$485.00 /Per Item
$949.00 /Per Item
25 µL $125.00
100 µg $485.00
500 µg $949.00
Availability: Ships next business day Availability: Ships next business day Availability: Ships next business day
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

Product Details

Anti-Collagen Type I (RABBIT) Antibody - 600-401-103-0.1
rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1
Rabbit
Polyclonal
IgG

Target Details

COL1A1/A2  - View All COL1A1/A2 Products
Human, Mouse, Rat, Bovine, Pig
Native Protein
Collagen Type I from human and bovine placenta
COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens.  Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.  This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
P08123 - UniProtKB
NP_000079.2 - NCBI Protein
1277 - Gene ID

Application Details

Dot Blot, IHC, WB
ELISA, FC, IF, IP, Multiplex  - View References
Anti-Collagen Type I has been tested by Western blot, dot blot, and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, IP, native PAGE, immunofluorescence, and FC for highly sensitive qualitative analysis.

Formulation

1.00 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Expiration date is one (1) year from date of receipt.
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(). Inhibition of lung microbiota-derived proapoptotic peptides ameliorates acute exacerbation of pulmonary fibrosis. Nature Communications
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(). Inhibition of Collagenase Q1 of Bacillus cereus as a Novel Antivirulence Strategy for the Treatment of Skin-Wound Infections. Advanced Therapeutics
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(). Regeneration of collagen fibrils at the papillary dermis by reconstructing basement membrane at the dermal–epidermal junction. Sci Rep.
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(). Red Light Mitigates the Deteriorating Placental Extracellular Matrix in Late Onset of Preeclampsia and Improves the Trophoblast Behavior. J Pregnancy.
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(). Addition of ROCK Inhibitors Alleviates Prostaglandin-Induced Inhibition of Adipogenesis in 3T3L-1 Spheroids. Bioengineering (Basel).
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(). Cancer cell survival depends on collagen uptake into tumor-associated stroma. NAt Commun.
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IF, Confocal Microscopy
(). Bioactive Cell-Derived ECM Scaffold Forms a Unique Cellular Microenvironment for Lung Tissue Engineering. Biomedicines.
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IF, Confocal Microscopy
(). Real-Time Measurement of Cell Mechanics as a Clinically Relevant Readout of an In Vitro Lung Fibrosis Model Established on a Bioinspired Basement Membrane. Adv MAter.
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(). All Trans-Retinoic Acids Facilitate the Remodeling of 2D and 3D Cultured Human Conjunctival Fibroblasts. Bioengineering (Basel).
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(). Bioprinting a Multifunctional Bioink to Engineer Clickable 3D Cellular Niches with Tunable Matrix Microenvironmental Cues. Adv Healthc Mater.
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Undefined
(). Emodin induces collagen type I synthesis in Hs27 human dermal fibroblasts. Experimental and Therapeutic Medicine
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WB, IB, PCA
(). Endothelial Klf2-Foxp1-TGFβ signal mediates the inhibitory effects of simvastatin on maladaptive cardiac remodeling. Theranostics.
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WB, IB, PCA
(). Connexin43 gap junction drives fascia mobilization and repair of deep skin wounds. Matrix Biol.
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IHC, ICC, Histology
(). Aortic carboxypeptidase-like protein regulates vascular adventitial progenitor and fibroblast differentiation through myocardin related transcription factor A. Sci Rep.
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WB, IB, PCA
(). Deletion of Myeloid Interferon Regulatory Factor 4 (Irf4) in Mouse Model Protects against Kidney Fibrosis after Ischemic Injury by Decreased Macrophage Recruitment and Activation. J Am Soc Nephrol.
Applications
Undefined
(). Trabecular meshwork's collagen network formation is inhibited by non-pigmented ciliary epithelium-derived extracellular vesicles. Journal of Cellular and Molecular Medicine
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WB, IB, PCA
(). Fibrocytes: A Critical Review and Practical Guide. Frontiers in Immunology
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WB, IB, PCA; FC, FACS, FLOW; IHC, ICC, Histology; IF, Confocal Microscopy
(). Imaging of type I procollagen biosynthesis in cells reveals biogenesis in highly organized bodies; Collagenosomes. Matrix Biol. Plus.
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IF, Confocal Microscopy; WB, IB, PCA
(). Development of a new decellularization protocol for the whole porcine heart. J Clin Transl Res.
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IF, Confocal Microscopy
(). Rewired glycosylation activity promotes scarless regeneration and functional recovery in spiny mice after complete spinal cord transection. Dev Cell.
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Undefined
(). Phenotypic drug screening in a human fibrosis model identified a novel class of antifibrotic therapeutics. Sci Adv.
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IF, Confocal Microscopy; WB, IB, PCA
(). Intermediary role of lung alveolar type 1 cells in epithelial repair upon Sendai virus infection. bioRxiv Preprint
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IF, Confocal Microscopy
(). Myocardin-related transcription factor A regulates integrin beta 2 transcription to promote macrophage infiltration and cardiac hypertrophy in mice. Cardiovasc Res.
Applications
Undefined
(). IL-1β-pre-conditioned mesenchymal stem/stromal cells' secretome modulates the inflammatory response and aggrecan deposition in intervertebral disc. Eur Cell Mater.
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IHC, ICC, Histology
(). Modelling human liver fibrosis in the context of non-alcoholic steatohepatitis using a microphysiological system. Commun Biol.
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IF, Confocal Microscopy
(). Suppression of the fibrotic encapsulation of silicone implants by inhibiting the mechanical activation of pro-fibrotic TGF-β. Nat Biomed Eng.
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Undefined
(). Disruption of CXCR6 Ameliorates Kidney Inflammation and Fibrosis in Deoxycorticosterone Acetate/Salt Hypertension. Sci Rep
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IF, Confocal Microscopy
(). Prostaglandin F2α agonist-induced suppression of 3T3-L1 cell adipogenesis affects spatial formation of extra-cellular matrix. Sci Rep
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IF, Confocal Microscopy
(). Silica-Based Nonwoven Fiber Fabricated by Electrospinning to Promote Fibroblast Functions. BCSJ
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E, EIA
(). Circulating fibrocytes can identify people with a poor prognosis in pulmonary fibrosis. medRxiv
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FC, FACS, FLOW
(). Role of the autotaxin-lysophosphatidic acid axis in glaucoma, aqueous humor drainage and fibrogenic activity. Biochim Biophys Acta Mol Basis Dis.
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WB, IB, PCA
(). Radhakrishnan RS. Burn-Induced Impairment of Ileal Muscle Contractility Is Associated with Increased Extracellular Matrix Components. J Gastrointest Surg.
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WB, IB, PCA
(). Microvascular engineering: Dynamic changes in microgel-entrapped vascular cells correlates with higher vasculogenic/angiogenic potential. Biomaterials
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IHC, ICC, Histology
(). Parenchymal pericytes are not the major contributor of extracellular matrix in the fibrotic scar after stroke in male mice. J Neurosci Res.
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IHC, ICC, Histology
(). Myocardin and myocardin-related transcription factor-A synergistically mediate actin cytoskeletal-dependent inhibition of liver fibrogenesis. Am J Physiol Gastrointest Liver Physiol.
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WB, IB, PCA
(). Exogenous supply of Hsp47 triggers fibrillar collagen deposition in skin cell cultures in vitro. BMC Mol Cell Biol.
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IF, Confocal Microscopy
(). L-Selectin/CD62L is a Key Driver of Non-Alcoholic Steatohepatitis in Mice and Men. Cells.
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WB, IB, PCA
(). In vitro analysis of the effect of Flightless I on murine tenocyte cellular functions. J Orthop Surg Res.
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IHC, ICC, Histology
(). Exercise-Induced Increases in Insulin Sensitivity After Bariatric Surgery Are Mediated By Muscle Extracellular Matrix Remodeling. Diabetes.
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IHC, ICC, Histology
(). Recombinant spider silk protein eADF4(C16)-RGD coatings are suitable for cardiac tissue engineering. Sci Rep.
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IF, Confocal Microscopy
(). Activation of TWIST Transcription by Chromatin Remodeling Protein BRG1 Contributes to Liver Fibrosis in Mice. Front Cell Dev Biol.
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WB, IB, PCA
(). Effects of pirfenidone targeting the tumor microenvironment and tumor-stroma interaction as a novel treatment for non-small cell lung cancer. Sci Rep.
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WB, IB, PCA; IF, Confocal Microscopy
(). AXL Is a Potential Target for the Treatment of Intestinal Fibrosis. Inflamm Bowel Dis.
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(). Dual roles of chromatin remodeling protein BRG1 in angiotensin II-induced endothelial-mesenchymal transition. Cell Death Dis.
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(). Attenuation of Flightless I Increases Human Pericyte Proliferation, Migration and Angiogenic Functions and Improves Healing in Murine Diabetic Wounds. Int J Mol Sci.
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IHC, ICC, Histology
(). CX3CR1-fractalkine axis drives kinetic changes of monocytes in fibrotic interstitial lung diseases. Eur Respir J.
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IF, Confocal Microscopy
(). Loss of HIF1A from pancreatic cancer cells increases expression of ppp1r1b and degradation of p53 to promote invasion and metastasis. Gastroenterology
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IHC, ICC, Histology
(). A Scaffold-Free 3-D Co-Culture Mimics the Major Features of the Reverse Warburg Effect In Vitro. Cells.
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IF, Confocal Microscopy
(). YAP Activation in Renal Proximal Tubule Cells Drives Diabetic Renal Interstitial Fibrogenesis. Diabetes.
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IF, Confocal Microscopy; IHC, ICC, Histology
(). Increasing the level of cytoskeletal protein Flightless I reduces adhesion formation in a murine digital flexor tendon model. J Orthop Surg Res.
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IHC, ICC, Histology
(). CILP1 as a biomarker for right ventricular maladaptation in pulmonary hypertension. Eur Respir J.
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IF, Confocal Microscopy
(). Systemic Delivery of Anti-integrin αL Antibodies Reduces Early Macrophage Recruitment, Inflammation, and Scar Formation in Murine Burn Wounds. Adv Wound Care (New Rochelle).
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IHC, ICC, Histology
(). Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin. Nat Commun.
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Undefined
(). Podocyte EGFR Inhibits Autophagy Through Upregulation of Rubicon in Type 2 Diabetic Nephropathy. Diabetes
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IHC, ICC, Histology
(). The intracellular chloride channel 4 (CLIC4) activates systemic sclerosis fibroblasts. Rheumatology (Oxford).
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WB, IB, PCA
(). Phospholipid Polymer Hydrogel Matrices with Dually Immobilized Cytokines for Accelerating Secretion of the Extracellular Matrix by Encapsulated Cells. Macromol Biosci.
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Undefined
(). Blockade of Platelet-Derived Growth Factor Signaling Inhibits Choroidal Neovascularization and Subretinal Fibrosis in Mice. Journal of Clinical Medicine
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(). Effects of ASC Application on Endplate Regeneration Upon Glycerol-Induced Muscle Damage. Frontiers in Molecular Neuroscience
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IF, Confocal Microscopy
(). Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids. Communications Biology
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IF, Confocal Microscopy
(). Clustering, Spatial Distribution, and Phosphorylation of Discoidin Domain Receptors 1 and 2 in Response to Soluble Collagen I. Journal of Molecular Biology
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E, EIA
(). HIF2A-LOX Pathway Promotes Fibrotic Tissue Remodeling in Thyroid-Associated Orbitopathy. Endocrinology.
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IF, Confocal Microscopy
(). Discovery and evaluation of inhibitor of LARP6 as specific antifibrotic compound. Sci Rep.
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WB, IB, PCA
(). Lkb1 deletion in periosteal mesenchymal progenitors induces osteogenic tumors through mTORC1 activation. J Clin Invest.
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IF, Confocal Microscopy
(). A non-autonomous role of MKL1 in the activation of hepatic stellate cells. Biochim Biophys Acta Gene Regul Mech.
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WB, IB, PCA
(). Functional Screening Identifies MicroRNAs as Multi-Cellular Regulators of Heart Failure. Sci Rep.
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WB, IB, PCA
(). Photoactivatable Hsp47: A Tool to Regulate Collagen Secretion and Assembly. Adv Sci (Weinh). 2019;6(9):1801982. Published 2019 Feb 28. doi:10.1002/advs.201801982 Adv Sci (Weinh).
Applications
WB, IB, PCA; IF, Confocal Microscopy
(). Collagen XIII-derived ectodomain regulates bone angiogenesis and intracortical remodeling. Matrix Biol.
Applications
Immuno-EM
(). Salvia miltiorrhiza and Carthamus tinctorius Extract Prevents Cardiac Fibrosis and Dysfunction after Myocardial Infarction by Epigenetically Inhibiting Smad3 Expression. Evid Based Complement Alternat Med.
Applications
WB, IB, PCA; IHC, ICC, Histology
(). The Role of the EGF Receptor in Sex Differences in Kidney Injury. J Am Soc Nephrol.
Applications
IHC, ICC, Histology
(). Intra-articularly injected mesenchymal stem cells promote cartilage regeneration, but do not permanently engraft in distant organs. Sci Rep.
Applications
IF, Confocal Microscopy
(). Smooth Muscle α-Actin Deficiency Leads to Decreased Liver Fibrosis via Impaired Cytoskeletal Signaling in Hepatic Stellate Cells Am J Pathol.
Applications
WB, IB, PCA
(). Mediation of the Acute Stress Response by the Skeleton. Cell Metab.
Applications
WB, IB, PCA
(). Transcriptome analysis reveals autophagy as regulator of TGFβ/Smad-induced fibrogenesis in trabecular meshwork cells. Sci Rep.
Applications
WB, IB, PCA
(). The Chromatin Remodeler Brg1 Integrates ROS Production and Endothelial-Mesenchymal Transition to Promote Liver Fibrosis in Mice. Front Cell Dev Biol. 2019;7:245. Published 2019 Oct 23. doi:10.3389/fcell.2019.00245
Applications
WB, IB, PCA
(). Lowering the culture temperature corrects collagen abnormalities caused by HSP47 gene knockout. Sci Rep.
Applications
IF, Confocal Microscopy; Multiplex Assay
(). MKL1 promotes endothelial-to-mesenchymal transition and liver fibrosis by activating TWIST1 transcription. Cell Death Dis.
Applications
WB, IB, PCA
(). Prion protein modulates endothelial to mesenchyme-like transition in trabecular meshwork cells: Implications for primary open angle glaucoma. Scientific Reports
Applications
WB, IB, PCA
(). Mouse placental scaffolds: a three-dimensional environment model for recellularization. Journal of Tissue Engineering
Applications
IHC, ICC, Histology
(). Effects of high glucose on proliferation and function of circulating fibrocytes: Involvement of CXCR4/SDF‑1 axis. International Journal of Molecular Medicine
Applications
IF, Confocal Microscopy
(). Suppression of Choroidal Neovascularization and Fibrosis by a Novel RNAi Therapeutic Agent against (Pro)renin Receptor. Molecular Therapy. Nucleic Acids
Applications
IF, Confocal Microscopy
(). Tubular NOX4 expression decreases in chronic kidney disease but does not modify fibrosis evolution. Redox Biology
Applications
WB, IB, PCA
(). Aortic carboxypeptidase-like protein enhances adipose tissue stromal progenitor differentiation into myofibroblasts and is upregulated in fibrotic white adipose tissue. PLOS One
Applications
WB, IB, PCA
(). Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells. Investigative Ophthalmology & Visual Science
Applications
WB, IB, PCA
(). Fatty acid receptor modulator PBI-4050 inhibits kidney fibrosis and improves glycemic control. JCI Insight
Applications
IHC, ICC, Histology
(). TGF-β promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration. JCI Insight
Applications
IHC, ICC, Histology
(). Myeloproliferative leukemia protein activation directly induces fibrocyte differentiation to cause myelofibrosis Leukemia.
Applications
IF, Confocal Microscopy
(). Guiding morphogenesis in cell-instructive microgels for therapeutic angiogenesis. Biomaterials.
Applications
IF, Confocal Microscopy
(). Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell.
Applications
IF, Confocal Microscopy
(). Fibrosis in Preeclamptic Placentas Is Associated with Stromal Fibroblasts Activated by the Transforming Growth Factor-β1 Signaling Pathway. Am J Pathol.
Applications
WB, IB, PCA
(). Distinct niches within the extracellular matrix dictate fibroblast function in (cell free) 3D lung tissue cultures. Am J Physiol Lung Cell Mol Physiol.
Applications
IHC, ICC, Histology
(). Cub domain-containing protein 1 negatively regulates TGF-β signaling and myofibroblast differentiation. Am J Physiol Lung Cell Mol Physiol.
Applications
WB, IB, PCA
(). Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes. Sci Rep.
Applications
IF, Confocal Microscopy
(). Improved Cardiovascular Function in Old Mice After N-Acetyl Cysteine and Glycine Supplemented Diet: Inflammation and Mitochondrial Factors. J Gerontol A Biol Sci Med Sci.
Applications
IF, Confocal Microscopy
(). Transcription factor 21 (TCF21) promotes proinflammatory interleukin 6 expression and extracellular matrix remodeling in visceral adipose stem cells. J Biol Chem.
Applications
IF, Confocal Microscopy
(). Sex steroids drive the remodeling of oviductal extracellular matrix in cattle. Biol Reprod.
Applications
IHC, ICC, Histology
(). Myocardin-Related Transcription Factor A Promotes Recruitment of ITGA5+ Profibrotic Progenitors during Obesity-Induced Adipose Tissue Fibrosis. Cell Rep.
Applications
WB, IB, PCA
(). Luteolin-Mediated Inhibition of Hepatic Stellate Cell Activation via Suppression of the STAT3 Pathway. Int J Mol Sci.
Applications
WB, IB, PCA
(). A Novel Microplate 3D Bioprinting Platform for the Engineering of Muscle and Tendon Tissues. SLAS Technol.
Applications
IHC, ICC, Histology
(). Cell-surface phenotyping identifies CD36 and CD97 as novel markers of fibroblast quiescence in lung fibrosis. Am J Physiol Lung Cell Mol Physiol.
Applications
IF, Confocal Microscopy; FC, FACS, FLOW
(). Inhibition of Epidermal Growth Factor Receptor Activation Is Associated With Improved Diabetic Nephropathy and Insulin Resistance in Type 2 Diabetes. Diabetes.
Applications
IHC, ICC, Histology
(). Differential effects of Nintedanib and Pirfenidone on lung alveolar epithelial cell function in ex vivo murine and human lung tissue cultures of pulmonary fibrosis. Respir Res.
Applications
WB, IB, PCA; IF, Confocal Microscopy
(). Equine lung decellularization: a potential approach for in vitro modeling the role of the extracellular matrix in asthma. J Tissue Eng.
Applications
IHC, ICC, Histology
(). Antifibrosis Effect of Novel Oridonin Analog CYD0618 Via Suppression of the NF-κB Pathway. J Surg Res.
Applications
WB, IB, PCA
(). Retrospective investigation of the prognostic value of the β1 integrin expression in patients with head and neck squamous cell carcinoma receiving primary radio(chemo)therapy. PLoS One.
Applications
IHC, ICC, Histology
(). Pirfenidone suppresses polarization to M2 phenotype macrophages and the fibrogenic activity of rat lung fibroblasts. J Clin Biochem Nutr.
Applications
WB, IB, PCA
(). Stromal hedgehog signaling maintains smooth muscle and hampers micro-invasive prostate cancer. Disease Models & Mechanisms
Applications
IHC, ICC, Histology
(). 3D pulmospheres serve as a personalized and predictive multicellular model for assessment of antifibrotic drugs. JCI Insight
Applications
IF, Confocal Microscopy
(). p21 maintains senescent cell viability under persistent DNA damage response by restraining JNK and caspase signaling. The EMBO Journal
Applications
WB, IB, PCA
(). Sox9 positive periosteal cells in fracture repair of the adult mammalian long bone. Bone
Applications
IHC, ICC, Histology
(). Altered paracrine signaling from the injured knee joint impairs postnatal long bone growth. Elife
Applications
IHC, ICC, Histology
(). Antifibrotic role of vascular endothelial growth factor in pulmonary fibrosis. JCI Insight
Applications
FC, FACS, FLOW
(). IL-4/IL-13-mediated polarization of renal macrophages/dendritic cells to an M2a phenotype is essential for recovery from acute kidney injury. Kidney Int.
Applications
IHC, ICC, Histology
(). Macrophage Cyclooxygenase-2 Protects Against Development of Diabetic Nephropathy. Diabetes. 2017;66(2):494-504. doi:10.2337/db16-0773 Diabetes.
Applications
IHC, ICC, Histology
(). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis Cell Stem Cell.
Applications
IHC, ICC, Histology; IF, Confocal Microscopy
(). Classical transient receptor potential 6 (TRPC6) channels support myofibroblast differentiation and development of experimental pulmonary fibrosis. Biochim Biophys Acta Mol Basis Dis.
Applications
IHC, ICC, Histology
(). HANAC Col4a1 Mutation in Mice Leads to Skeletal Muscle Alterations due to a Primary Vascular Defect. Am J Pathol.
Applications
IF, Confocal Microscopy
(). mTORC1 phosphorylates LARP6 to stimulate type I collagen expression. Sci Rep.
Applications
WB, IB, PCA
(). Transcriptome-based repurposing of apigenin as a potential anti-fibrotic agent targeting hepatic stellate cells. Sci Rep.
Applications
WB, IB, PCA
(). An ex vivo model to induce early fibrosis-like changes in human precision-cut lung slices Am J Physiol Lung Cell Mol Physiol.
Applications
WB, IB, PCA
(). Regeneration of subcutaneous tissue-engineered mandibular condyle in nude mice. J Craniomaxillofac Surg.
Applications
IHC, ICC, Histology
(). Dissecting the role of myeloid and mesenchymal fibroblasts in age-dependent cardiac fibrosis. Basic Res Cardiol.
Applications
IF, Confocal Microscopy; FC, FACS, FLOW
(). CMG2/ANTXR2 regulates extracellular collagen VI which accumulates in hyaline fibromatosis syndrome. Nat Commun.
Applications
E, EIA; WB, IB, PCA
(). Lysophosphatidic Acid Receptor Antagonism Protects against Diabetic Nephropathy in a Type 2 Diabetic Model. J Am Soc Nephrol.
Applications
IHC, ICC, Histology
(). A study to identify and characterize the stem/progenitor cell in rabbit meniscus. Cytotechnology
Applications
IHC, ICC, Histology
(). Akt mediated phosphorylation of LARP6; critical step in biosynthesis of type I collagen. Scientific Reports
Applications
WB, IB, PCA
(). Vitronectin-Based, Biomimetic Encapsulating Hydrogel Scaffolds Support Adipogenesis of Adipose Stem Cells. Tissue Engineering - Part A
Applications
IF, Confocal Microscopy; IHC, ICC, Histology
(). Zoledronic acid suppresses transforming growth factor-β-induced fibrogenesis by human gingival fibroblasts. International Journal of Molecular Medicine
Applications
IF, Confocal Microscopy
(). Mesenchymal Stem/Stromal Cells seeded on cartilaginous endplates promote Intervertebral Disc Regeneration through Extracellular Matrix Remodeling. Scientific Reports
Applications
IHC, ICC, Histology
(). COMP-assisted collagen secretion--a novel intracellular function required for fibrosis. J Cell Sci.
Applications
IF, Confocal Microscopy
(). Continuous Grading of Early Fibrosis in NAFLD Using Label-Free Imaging: A Proof-of-Concept Study. PLoS One.
Applications
IF, Confocal Microscopy
(). Surface proteome analysis identifies platelet derived growth factor receptor-alpha as a critical mediator of transforming growth factor-beta-induced collagen secretion. Int J Biochem Cell Biol.
Applications
WB, IB, PCA
(). Cardiac-restricted Overexpression of TRAF3 Interacting Protein 2 (TRAF3IP2) Results in Spontaneous Development of Myocardial Hypertrophy, Fibrosis, and Dysfunction. J Biol Chem.
Applications
IF, Confocal Microscopy; Multiplex Assay
(). Dynamic remodeling of endometrial extracellular matrix regulates embryo receptivity in cattle Reproduction.
Applications
IHC, ICC, Histology
(). A Bioinspired Ultraporous Nanofiber-Hydrogel Mimic of the Cartilage Extracellular Matrix. Adv Healthc Mater.
Applications
IHC, ICC, Histology
(). Exploring the in vivo wound healing effects of a recombinant hemolin from the caterpillar Lonomia obliqua. J Venom Anim Toxins Incl Trop Dis.
Applications
IHC, ICC, Histology
(). STAT3 Inhibition Suppresses Hepatic Stellate Cell Fibrogenesis: HJC0123, a Potential Therapeutic Agent for Liver Fibrosis. RSC Adv.
Applications
WB, IB, PCA; IF, Confocal Microscopy
(). A novel profibrotic mechanism mediated by TGFβ-stimulated collagen prolyl hydroxylase expression in fibrotic lung mesenchymal cells. The Journal of Pathology
Applications
WB, IB, PCA
(). Second-generation compound for the modulation of utrophin in the therapy of DMD. Human Molecular Genetics
Applications
IF, Confocal Microscopy
(). β1 Integrins Mediate Attachment of Mesenchymal Stem Cells to Cartilage Lesions. BioResearch Open Access
Applications
IHC, ICC, Histology
(). CD8+ T Cell Response to Gammaherpesvirus Infection Mediates Inflammation and Fibrosis in Interferon Gamma Receptor-Deficient Mice. PLOS One
Applications
IF, Confocal Microscopy
(). Endothelial-to-mesenchymal transition drives atherosclerosis progression. Journal of Clinical Investigation
Applications
IHC, ICC, Histology
(). Interaction of Biomechanics with Extracellular Matrix Components in Abdominal Aortic Aneurysm Wall. Eur J Vasc Endovasc Surg.
Applications
IHC, ICC, Histology
(). Myocardial healing requires Reg3β-dependent accumulation of macrophages in the ischemic heart. Nat Med.
Applications
IF, Confocal Microscopy
(). Multidimensional immunolabeling and 4D time-lapse imaging of vital ex vivo lung tissue. Am J Physiol Lung Cell Mol Physiol.
Applications
IF, Confocal Microscopy
(). Assessing the accuracy and reproducibility of modality independent elastography in a murine model of breast cancer. J Med Imaging (Bellingham).
Applications
IHC, ICC, Histology
(). Rapamycin increases CCN2 expression of lung fibroblasts via phosphoinositide 3-kinase. Lab Invest.
Applications
WB, IB, PCA
(). MKL1 is an epigenetic modulator of TGF-β induced fibrogenesis. Biochim Biophys Acta.
Applications
WB, IB, PCA
(). Enhanced anti-fibrogenic effects of novel oridonin derivative CYD0692 in hepatic stellate cells. Mol Cell Biochem.
Applications
WB, IB, PCA
(). Enhanced effects of novel oridonin analog CYD0682 for hepatic fibrosis. J Surg Res.
Applications
WB, IB, PCA
(). MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice. Proc Natl Acad Sci U S A.
Applications
IHC, ICC, Histology
(). Long-term type 1 diabetes alters the deposition of collagens and proteoglycans in the early pregnant myometrium of mice. Favaro RR, Raspantini PR, Salgado RM, Fortes ZB, Zorn TM.
Applications
IF, Confocal Microscopy
(). Inhibition of choroidal fibrovascular membrane formation by new class of RNA interference therapeutic agent targeting periostin. Gene Ther.
Applications
IHC, ICC, Histology
(). Patient-derived skeletal dysplasia induced pluripotent stem cells display abnormal chondrogenic marker expression and regulation by BMP2 and TGFβ1. Stem Cells Dev.
Applications
IF, Confocal Microscopy; WB, IB, PCA
(). Identification of a collagen type I adhesin of Bacteroides fragilis. PLoS One.
Applications
WB, IB, PCA
(). FOXD1 promotes nephron progenitor differentiation by repressing decorin in the embryonic kidney. Development.
Applications
IHC, ICC, Histology
(). A novel murine model of hypertrophic scarring using subcutaneous infusion of bleomycin. Plast Reconstr Surg.
Applications
IHC, ICC, Histology
(). 3-D adipocyte differentiation and peri-adipocyte collagen turnover. Methods Enzymol.
Applications
IHC, ICC, Histology; IF, Confocal Microscopy
(). Role of adenosine A2B receptor signaling in contribution of cardiac mesenchymal stem-like cells to myocardial scar formation. Purinergic Signal.
Applications
WB, IB, PCA; FC, FACS, FLOW
(). AMP-activated protein kinase regulates intraocular pressure, extracellular matrix, and cytoskeleton in trabecular meshwork. Invest Ophthalmol Vis Sci.
Applications
WB, IB, PCA
(). Oridonin inhibits hepatic stellate cell proliferation and fibrogenesis. J Surg Res.
Applications
WB, IB, PCA
(). TGF-β2-mediated ocular hypertension is attenuated in SPARC-null mice. Invest Ophthalmol Vis Sci.
Applications
IHC, ICC, Histology
(). miR-92a regulates TGF-β1-induced WISP1 expression in pulmonary fibrosis. Int J Biochem Cell Biol.
Applications
WB, IB, PCA
(). Stk11 (Lkb1) deletion in the osteoblast lineage leads to high bone turnover, increased trabecular bone density and cortical porosity. Bone.
Applications
IF, Confocal Microscopy
(). Megakaryocytic leukemia 1 (MKL1) regulates hypoxia induced pulmonary hypertension in rats. PLoS One.
Applications
WB, IB, PCA
(). Resveratrol inhibits collagen I synthesis by suppressing IGF-1R activation in intestinal fibroblasts. World J Gastroentrol.
Applications
WB, IB, PCA
(). The effects of fibrin and fibrin-agarose on the extracellular matrix profile of bioengineered oral mucosa. J Tissue Eng Regen Med.
Applications
IHC, ICC, Histology
(). IQGAP1 suppresses TβRII-mediated myofibroblastic activation and metastatic growth in liver. J Clin Invest.
Applications
WB, IB, PCA
(). Implicating exudate macrophages and Ly-6C(high) monocytes in CCR2-dependent lung fibrosis following gene-targeted alveolar injury. J Immunol.
Applications
FC, FACS, FLOW; IHC, ICC, Histology
(). AICAR-dependent AMPK activation improves scar formation in the aged heart in a murine model of reperfused myocardial infarction. J Mol Cell Cardiol.
Applications
FC, FACS, FLOW
(). Ascorbate enhances elastin synthesis in 3D tissue-engineered pulmonary fibroblasts constructs. Tissue & Cell
Applications
IHC, ICC, Histology
(). CXC-type chemokines promote myofibroblast phenoconversion and prostatic fibrosis. PLoS One.
Applications
FC, FACS, FLOW; Multiplex Assay
(). Lanthanum carbonate, like sevelamer-HCl, retards the progression of vascular calcification and atherosclerosis in uremic apolipoprotein E-deficient mice. Nephrol Dial Transplant.
Applications
IHC, ICC, Histology
(). Histone methyltransferase ASH1 orchestrates fibrogenic gene transcription during myofibroblast transdifferentiation. Hepatology.
Applications
IHC, ICC, Histology
(). Proteoglycan and collagen expression during human air conducting system development. Eur J Histochem.
Applications
IHC, ICC, Histology
(). Hepatic Steatosis, Fibrosis, and Cancer in Elderly Cadavers. Cancer Biology
Applications
IHC, ICC, Histology
(). High and low molecular weight hyaluronic acid differentially regulate human fibrocyte differentiation. PloS One
Applications
IHC, ICC, Histology
(). Syndecan-4 is essential for development of concentric myocardial hypertrophy via stretch-induced activation of the calcineurin-NFAT pathway. PLoS One.
Applications
IHC, ICC, Histology
(). Coordinated regulation of extracellular matrix synthesis by the microRNA-29 family in the trabecular meshwork. Invest Ophthalmol Vis Sci.
Applications
WB, IB, PCA
(). Dynamic mechanical stimulations induce anisotropy and improve the tensile properties of engineered tissues produced without exogenous scaffolding. Acta Biomater.
Applications
WB, IB, PCA
(). Globular domain of adiponectin: promising target molecule for detection of atherosclerotic lesions. Biologics : Targets & Therapy
Applications
IF, Confocal Microscopy
(). Improved serum-free culture conditions for spleen-derived murine fibrocytes. J Immunol Methods
Applications
IHC, ICC, Histology; FC, FACS, FLOW
(). Characterization of differential properties of rabbit tendon stem cells and tenocytes. BMC Musculoskelet Disord.
Applications
IHC, ICC, Histology
(). Distinct types of fibrocyte can differentiate from mononuclear cells in the presence and absence of serum. PLoS One.
Applications
IF, Confocal Microscopy
(). Neuropilin-1 promotes cirrhosis of the rodent and human liver by enhancing PDGF/TGF-beta signaling in hepatic stellate cells. J Clin Invest.
Applications
WB, IB, PCA
(). Calcium concrements in the pineal gland of the Arctic fox (Vulpes lagopus) and their relationship to pinealocytes, glial cells and type I and III collagen fibers. Pol J Vet Sci.
Applications
IHC, ICC, Histology
(). Platelet-rich plasma releasate promotes differentiation of tendon stem cells into active tenocytes. Am J Sports Med.
Applications
WB, IB, PCA; IF, Confocal Microscopy
(). The effects of aging on the molecular and cellular composition of the prostate microenvironment. PLoS One.
Applications
IF, Confocal Microscopy
(). Genetic deletion of the angiotensin-(1-7) receptor Mas leads to glomerular hyperfiltration and microalbuminuria. Kidney Int.
Applications
IHC, ICC, Histology
(). VEGF ameliorates pulmonary hypertension through inhibition of endothelial apoptosis in experimental lung fibrosis in rats. J Clin Invest.
Applications
IF, Confocal Microscopy
(). Improved serum-free culture conditions for the differentiation of human and murine fibrocytes. J Immunol Methods.
Applications
IHC, ICC, Histology
(). Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation. J Leukoc Biol.
Applications
IHC, ICC, Histology
(). Adenoviral overexpression and small interfering RNA suppression demonstrate that plasminogen activator inhibitor-1 produces elevated collagen accumulation in normal and keloid fibroblasts. Am J Pathol.
Applications
WB, IB, PCA
(). Major histocompatibility class II transactivator expression in smooth muscle cells from A2b adenosine receptor knock-out mice. J Biol Chem.
Applications
WB, IB, PCA
(). Collagen-based fibrous scaffold for spatial organization of encapsulated and seeded human mesenchymal stem cells. Biomaterials
Applications
Quantum Dot Labeling (Q-Dot)
(). Dual effects of the membrane-anchored MMP regulator RECK on chondrogenic differentiation of ATDC5 cells. J Cell Sci.
Applications
IF, Confocal Microscopy; Multiplex Assay
(). High glucose activates rat pancreatic stellate cells through protein kinase C and p38 mitogen-activated protein kinase pathway. Pancreas
Applications
WB, IB, PCA
(). The anchorless adhesin Eap (extracellular adherence protein) from Staphylococcus aureus selectively recognizes extracellular matrix aggregates but binds promiscuously to monomeric matrix macromolecules. Matrix Biol.
Applications
WB, IB, PCA
(). Site-specific in vivo calcification and osteogenesis stimulated by bone sialoprotein. Calcif Tissue Int.
Applications
WB, IB, PCA
(). Adipogenic transcriptional regulation of hepatic stellate cells. J. Biol. Chem.
Applications
WB, IB, PCA
(). Inhibitory effect of dicationic diphenylfurans on production of type I collagen by human fibroblasts and activated hepatic stellate cells. Life Sci.
Applications
WB, IB, PCA
(). Peroxisome Proliferator-activated Receptor gamma Induces a Phenotypic Switch from Activated to Quiescent Hepatic Stellate Cells. J. Biol. Chem.
Applications
WB, IB, PCA
(). TRAM2 protein interacts with endoplasmic reticulum Ca2+ pump Serca2b and is necessary for collagen type I synthesis. Mol Cell Biol.
Applications
IP, Co-IP; WB, IB, PCA
(). 5' stem-loop of collagen alpha 1(I) mRNA inhibits translation in vitro but is required for triple helical collagen synthesis in vivo. J Biol Chem.
Applications
WB, IB, PCA
(). Inhibition of collagen alpha 1(I) expression by the 5' stem-loop as a molecular decoy. J.Biol. Chem.
Applications
WB, IB, PCA
(). The PERK eukaryotic initiation factor 2α kinase is required for the development of the skeletal system, postnatal growth, and the function and viability of the pancreas. Mol Cell Biol.
Applications
WB, IB, PCA
(). The membrane-anchored MMP inhibitor RECK is a key regulator of extracellular matrix integrity and angiogenesis. Cell
Applications
IHC, ICC, Histology
(). Association of the developing acrosome with multiple small Golgi units, the Golgi satellites, in spermatids of the musk shrew, Suncus murinus. J Reprod Fertil.
Applications
IHC, ICC, Histology

What were the immunogens for the collagen antibodies?

The immunogens for the collagen antibodies are the respective purified solubilized native collagen proteins (tropocollagens).

In what format are the collagen antibodies provided?

The collagen antibodies are provided in liquid format at a concentration of around 1 mg/mL (Absorbance 280 nm).

In what buffer are the collagen antibodies provided?

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide.

Are the antibodies specific to the reduced or non-reduced (native) proteins?

The collagen antibodies are specific to the native 3D epitopes found on the collagen native collagen protein. The collagen antibodies may have no reactivity to reduced collagen.

How was the reactivity of the antibodies tested? Was cross-reactivity to other collagens tested?

Each antibody was tested by immunoblot against each type of collagen (Type I – VI). Due to the close association of collagen proteins, cross-reactivity to other collagens may be observed. The optimal experimental conditions should be determined to minimize cross-reactivity in your application.

What are the recommended storage conditions for collagen antibodies?

The recommended storage temperature for collagen antibodies is -20°C. Please be sure to add glycerol 1:1 before freezing. We recommend making aliquots for best stability. The antibodies are stable for several weeks at 4°C.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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