Collagen Type I Antibody Biotin Conjugated
FLOW Cytometry of Anti Collagen Type I Biotin Conjugated
Flow Cytometry - Anti-Collagen 1 Biotin
Immunohistochemistry - Anti-Collagen Type I Antibody
Immunohistochemistry - Anti-Collagen Type I Antibody
Anti-Collagen I Anitbody - Western Blot
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Datasheet

Collagen Type I Antibody Biotin Conjugated

Rabbit Polyclonal

600-406-103
100 µg
Dot Blot, FC, IF, IHC, WB
Human, Mouse, Rat
Rabbit
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$469.00 /Per Item
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Description

Background

COLLAGEN Type I Antibody Biotin Conjugated is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.

Application Note

Anti-COLLAGEN Type I Antibody Biotin Conjugated has been tested by dot blot and Flow Cytometry and is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

Purity/Specificity

This product has been prepared by immunoaffinity chromatography using immobilized antigens.  Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.  This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

Anti-Collagen Type I (RABBIT) Antibody Biotin Conjugated - 600-406-103
rabbit anti-collagen type I antibody biotin conjugation, biotin conjugated rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1
COL1A1
Dot Blot, FC
IF, IHC, WB
Human, Mouse, Rat
Rabbit
Biotin
Polyclonal
IgG
Native Protein
Collagen Type I from human and bovine placenta.
10-20 moles BAC per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.   Expiration date is one (1) year from date of restoration.
Expiration date is one (1) year from date of receipt.
Inhibition of lung microbiota-derived proapoptotic peptides ameliorates acute exacerbation of pulmonary fibrosis. Nature Communications ()
Applications
EIA, ELISA
Blockade of PD-1/PD-L1 Pathway Enhances the Antigen-Presenting Capacity of Fibrocytes. J Immunol. ()
Applications
FC, FACS, FLOW
Fibrocytes: A Critical Review and Practical Guide. Frontiers in Immunology ()
Applications
IHC, ICC, Histology; FC, FACS, FLOW
A high-content, in vitro cardiac fibrosis assay for high-throughput, phenotypic identification of compounds with anti-fibrotic activity. J Mol Cell Cardiol. ()
Applications
Undefined
Effect of Orento, a Traditional Japanese Medicine, on IL-6, IL-8 Secretion, Type 1 Collagen Production and Alkaline Phosphatase Secretion in the Human Osteosarcoma Cell Line Saos-2. Medicines (Basel, Switzerland) ()
Applications
EIA, ELISA
Monocyte-derived fibrocytes elimination had little contribution on liver fibrosis. Hepatobiliary Pancreat Dis Int. ()
Applications
Undefined
S100A4 protein is essential for the development of mature microfold cells in Peyer's patches. Cell Rep. ()
Applications
IHC, ICC, Histology
Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis. Respiratory Research ()
Applications
FC, FACS, FLOW
Vaccinia vaccine-based immunotherapy arrests and reverses established pulmonary fibrosis. JCI Insight ()
Applications
IHC, ICC, Histology
Cortactin promotes exosome secretion by controlling branched actin dynamics. Journal of Cell Biology ()
Applications
WB, IB, PCA
MicroRNA-29c regulates apoptosis sensitivity via modulation of the cell-surface death receptor, Fas, in lung fibroblasts. Am J Physiol Lung Cell Mol Physiol. ()
Applications
WB, IB, PCA
ZBTB17 (MIZ1) Is Important for the Cardiac Stress Response and a Novel Candidate Gene for Cardiomyopathy and Heart Failure. Circulation: Cardiovascular Genetics ()
Applications
IF, Confocal Microscopy
Fibrocytes represent a novel MDSC subset circulating in patients with metastatic cancer. Blood ()
Applications
FC, FACS, FLOW
CXC-type chemokines promote myofibroblast phenoconversion and prostatic fibrosis. PLoS One. ()
Applications
IF, Confocal Microscopy
Bone marrow–derived progenitor cells in pulmonary fibrosis. Journal of Clinical Investigation ()
Applications
IHC, ICC, Histology; FC, FACS, FLOW

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.