Human CXCL11 - I-TAC ELISA Kit
Human CXCL11/I-TAC Accusignal ELISA Kit
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Datasheet

Human CXCL11 - I-TAC ELISA Kit

KOA0431
1 Kit
ELISA
Human
62.5 pg/ml - 4000 pg/ml
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Description

Background

Chemokine(C-X-C motif) ligand 11(CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant(I-TAC) and Interferon-gamma-inducible protein 9(IP-9). By PCR analysis of somatic cell hybrids, the CXCL11 gene was mapped to chromosome 4, and using FISH, it was located to 4q21.2. The CXCL11 was strongly induced by IFNG and beta-interferon, but only weakly induced by alpha-interferon. Induction of CXCL11 required expression of STAT1. What's more, it found that monocyte chemotaxis occurs in response to recombinant CXCL11. RT-PCR analysis detected CXCL11 expression in brain tissue from a patient with AIDS dementia and a patient with multiple sclerosis but not in 2 controls.

Application Note

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human CXCL11 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CXCL11 standard solution and each sample be measured in duplicate.

Purity/Specificity

Natural and recombinant human CXCL11. There is no detectable cross-reactivity with other relevant proteins.

Human CXCL11/I-TAC AccuSignal ELISA Kit - KOA0431
b R1|Beta R1, C X C motif chemokine 11, C-X-C motif chemokine 11, Chemokine C-X-C motif ligand 11, CXL11_HUMAN, H174, I TAC, Interferon gamma inducible protein 9, Interferon inducible T cell a chemoattractant I TAC, Interferon inducible T cell alpha chemoattractant, IP9, ITAC, SCYB11, SCYB9B, Small inducible cytokine B11, Small inducible cytokine subfamily B member 11, Small inducible cytokine subfamily B member 9B, Small inducible cytokine subfamily B member 11, Small inducible cytokine subfamily B member 9B
CXCL11
ELISA
Human
Expression system for standard: E.coli; Immunogen sequence: F22-F94
ELISA Kit
62.5 pg/ml - 4000 pg/ml
<10 pg/ml
Heparin Sodium
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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