APG8 Control Lysate
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Datasheet

APG8 Control Lysate

W00-001-P09
200 µg
E. coli
This product is discontinued

Background

Ready-to-use positive control lysates produced by Rockland Immunochemicals are derived from bacterial strains, cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to the relevant epitope(s) that confirm the presence of the protein(s) of interest. This product is intended as a positive control for use with Rockland Immunochemicals anti-APG8 antibodies.

Product Details

APG8 Control Lysate - W00-001-P09
APG8, AUT7, CVT5, APG8 Positive Control Lysate
Other
E. coli

Target Details

ATG8
Following induction for expression of the recombinant protein, E. coli culture was pelleted by centrifugation and resuspended in lysis buffer containing protease inhibitors. The cells were lysed using enzymatic lysis and sonication. The insoluble fraction of the lysate was subsequently separated from the soluble fraction using centrifugation and a sample of the soluble fraction was used to determine protein concentration by Lowry assay with a commercially available kit. The protein concentration was adjusted to 2 mg/ml and then an equal volume of 2X SDS-PAGE sample buffer was added.

Application Details

Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). If dissociating conditions are desired, add reducing agent prior to heating. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2.0 µL is recommended for SDS-PAGE. The product is supplied in 1X loading buffer and is ready-to-use.

Cell Line Data

Whole Cell Lysate
Not Stimulated
None (Control)

Formulation

Liquid (sterile filtered)
1.0 mg/mL by modified Lowry assay
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
None
10% (v/v) Glycerol

Shipping & Handling

Dry Ice
Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
Expiration date is three (3) months from date of receipt.

Invalid lot number

If you need help finding your CoA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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