HER2 Signaling Antibodies


Human epidermal growth factor receptor 2 (HER2) is a member of the epidermal growth factor receptor (HER/EGFR/ERBB) family. It is also called ERBB2, CD340, or proto-oncogene Neu. The protein is a receptor tyrosine kinase located on the plasma membrane of cells. Activation of the tyrosine kinase promotes cell proliferation and suppresses apoptosis. HER2 can dimerize with any other member of the ErbB family, which results in auto-phosphorylation of tyrosine residues and activation of signaling pathways. The signal is then transferred via PI3K/Akt, RAS/MEK/MAPK, and STAT kinase that can be detected using our phospho-specific antibodies to accurately determine activation of the pathway.

Her2 Signaling Pathway

Figure: Her2 signaling pathway (Created with BioRender.com)

HER2 is over-expressed in a significant proportion of cancer patients resulting in an increased disease recurrence and poor prognosis. Therefore, an early diagnosis of the HER2 status in the tumors is necessary to decide the appropriate treatment. Furthermore, in breast cancer, HER2 is over-expressed in a variety of different tumors which makes it a target for immunotherapy of certain patients. The antibody Trastuzumab (Herceptin) which recognizes HER2 was approved for the treatment of HER2-positive breast cancer patients in 1998, as the original approval was for the treatment of HER2-positive metastatic breast tumors. Later studies have shown that Herceptin is also beneficial for early-stage HER2-positive tumor patients and is approved as an adjuvant treatment. Additionally, the antibody is now approved for other HER2-positive tumors, such as metastatic gastric adenocarcinoma.

Rockland's antibodies exhibit high specificity and selectivity for the detection of HER2, HER2 ligands, and key proteins of the affected pathways and are routinely used for reproducible detection by Western blotting, immunohistochemistry, immunofluorescence, and ELISA. Accurate detection and quantification of HER2 in complex matrices, including cell culture supernatant, serum or plasma, and tissue homogenates, can be achieved using our Human ErbB-2 AccuSignal ELISA Kit.


Featured Product

Product Clonality Reactivity Application
Recombinant Anti-HER2 Fab Antibody Monoclonal Human WB, FC, ELISA


Her2 Signaling Pathway Antibodies

Product Clonality Reactivity Application
AKT phospho S473 Antibody Polyclonal Human, Mouse, Rat WB, IHC, IF, ELISA
AKT phospho S473 Antibody Monoclonal Human, Mouse, Rat, Monkey WB, IHC, IF, ELISA
Recombinant Anti-HER2 Fab Antibody Monoclonal Human WB, FC, ELISA
HER3 Antibody Polyclonal Human WB, IP, ELISA
ERK2 C-Term Antibody Polyclonal Human, Mouse, Rat WB, ELISA
MEK1 C-Term Antibody Polyclonal Human, Mouse, Rat WB, IHC, ELISA
MEK1 C-Term Antibody Monoclonal Human, Mouse, Rat WB, IP, ELISA
p70 S6 Kinase Antibody Polyclonal Mouse WB, IP, ELISA
STAT2 phospho Y690 Antibody Polyclonal Human, Mouse WB, IHC, ELISA


Her2 Signaling Pathway Proteins

Product Host Origin Application
AKT1 Human Recombinant Protein Baculovirus Human WB, SDS-PAGE
MEK1 human recombinant protein Sf9 Cells Human WB, SDS-PAGE
PI3K (p55 gamma) protein-HIS Epitope Insect Cells Human WB, SDS-PAGE
RAS protein-GST fusion E.coli Human WB, Kinase Assay