HER2 Signaling Antibodies

 

HER2 (human epidermal growth factor receptor 2) is a member of the epidermal growth factor receptor (HER/EGFR/ERBB) family. It is also called ERBB2, CD340, or proto-oncogene Neu. The protein is a receptor tyrosine kinase located on the plasma membrane of cells. Activation of the tyrosine kinase promotes cell proliferation and suppresses apoptosis. HER2 can dimerize with any other member of the ErbB family, which results in auto-phosphorylation of tyrosine residues and activation of signaling pathways. The signal is then transferred via PI3K/Akt, RAS/MEK/MAPK, and STAT kinase that can be detected using our phospho-specific antibodies to accurately determine activation of the pathway. Based on the Human Protein Reference Database (HPRD), HER2 interacts with more than 50 proteins directly.

HER2 is over-expressed in a significant proportion of cancer patients resulting in an increased disease recurrence and poor prognosis. Therefore an early diagnosis of the HER2 status in the tumors is necessary to decide the appropriate treatment. Furthermore, in breast cancer, HER2 is over-expressed in a variety of different tumors which makes it a target for immunotherapy of certain patients. The antibody Trastuzumab (Herceptin) which recognizes HER2 was approved for the treatment of HER2-positive breast cancer patients in 1998. The original approval was for the treatment of HER2-positive metastatic breast tumors. Later studies have shown that Herceptin is also beneficial for early-stage HER2-positive tumor patients and is approved as an adjuvant treatment. Additionally, the antibody is now approved also for other HER2-positive tumors (metastatic gastric adenocarcinoma).

Rockland's antibodies exhibiting high specificity and selectivity for the detection of HER2, HER2 ligands, and key proteins of the affected pathways are routinely used for reproducible detection by Western blotting, Immunohistochemistry, Immunofluorescence, and ELISA. Accurate detection and quantification of HER2 in complex matrices–including cell culture supernatant, serum or plasma, and tissue homogenates—can be achieved using our Human ErbB-2 AccuSignal ELISA Kit.

Her2 Signaling Pathway Antibodies
  • Akt phospho S473 Antibody
  • MEK1 C-Term Antibody
  • ERK2 C-Term Antibody
  • p70 S6 Kinase Antibody
  • STAT2 phospho Y690 Antibody
Her2 Signaling Pathway Proteins
  • AKT1 Human Recombinant Protein
  • PI3K (p55 gamma) protein-HIS Epitope
  • MEK1 Human Recombinant Protein