Vice President of Research and Development
David is currently serving as the Vice President of Research and Development at Rockland Immunochemicals and has more than 15 years of academic and industrial experience. David oversees custom projects and contract research services and works to bridge client needs and laboratory efforts with biotech, large pharmaceutical companies, and academic laboratories. David works with a team of world-class Rockland scientists and technicians in custom polyclonal and monoclonal antibody generation, immunoassay development, and molecular biology services. Additionally, he leads Rockland’s efforts on government-funded SBIR grants and contracts using innovation to create a lasting impact on healthcare problems.
David has a Doctorate in Microbiology and Immunology from the University of Virginia and received postdoctoral training in Biochemistry and Biophysics at the University of Pennsylvania in Philadelphia. David has worked extensively in utilizing protein structural and bioinformatics analyses to design highly specialized research antibodies.
Facts About Me
- I have a Doctorate in Microbiology and Immunology from the University of Virginia
- I received postdoctoral training in Biochemistry and Biophysics at the University of Pennsylvania in Philadelphia
- I have utilized protein structural and bioinformatics analyses to design highly specialized research antibodies
- I enjoy coaching youth girls lacrosse for my daughters
- I grow a variety of peppers and like to experiment creating different hot sauces
Top Skills
- Antibody Generation
- Assay Development
- Microbiology
- Protein Biochemistry
- Protein Expression & Purification
Articles
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Creation of a broadly reactive E. coli HCP detection ELISA suitable for K Strain and B Strain Platforms
Learn more about a new E. coli HCP ELISA for accurate monitoring in biopharmaceutical production, ensuring consistency in recombinant protein and plasmid workflows. -
25 years of NF-κB at Rockland
We look at the antibodies we produced against NF-κB, its phosphorylated forms, subunits, inhibitors and NLS sites using new technologies. -
Novel Multiplex Test for Lyme Disease
This poster presents the preliminary data in Rocklands efforts to develop a POC testing device for Lyme Disease. -
Improved Western Blot Assays Using BlotCycler™ Automated Wash System
Comparing traditional Western blotting and automatic Western blotting by using Rockland's DyLight™ fluorochrome conjugated secondary antibodies. -
Multi-assay Functional Antibodies in HCP Detection
This poster presents methods and data for HCP antibody production using antigens from different hosts using different HCP sample types. -
Enhanced HCP Coverage Analysis utilizing Multiplexed 2D Electrophoresis
Evaluate the coverage of a generic anti-CHO-HCP antibody (Rockland Inc.) by 2D-PAGE separation using two experimental methods. -
Development of an improved standardized immunoassay for HCP detection using 2D electrophoresis and western blotting system
Development of an improved standardized immunoassay for HCP detection using 2D electrophoresis and western blotting system -
HCP Analysis of a Small Drug-Protein in Process Sample
Poster exploring a combination of analysis methods enabling maximum coverage and information details on each HCP. -
H6D Polymorphism Specific Detection of NAG1 in Human Serum
The goal is to create a commercial ELISA with low background and high signal-to-noise ratio that's capable of detecting serum NAG1 at concentrations of ≥25 pg/mL. -
Enhanced Multiplex Western Blotting
Enhanced multiplex fluorescent western blotting protocol for the production of immunoblot on PVDF membrane and determining limit of fluorescent detection. -
Detection of Akt Migration Using STED Nanoscopy
Scientific Poster Discussing the analysis of of the sub-cellular localization of Akt using both standard confocal microscopy and high-res STED Nanoscopy.
Publications
- Yukhananov, R., Chimento, D. P., & Marlow, L. A. (2022). Western Blot Processing Optimization: The Perfect Blot. Methods in molecular biology (Clifton, N.J.)
- Chimento, D. P., Kadner, R. J., & Wiener, M. C. (2005). Comparative structural analysis of TonB-dependent outer membrane transporters: implications for the transport cycle. Proteins
- Chimento, D. P., Kadner, R. J., & Wiener, M. C. (2003). The Escherichia coli outer membrane cobalamin transporter BtuB: structural analysis of calcium and substrate binding, and identification of orthologous transporters by sequence/structure conservation. Journal of molecular biology
- Chimento, D. P., Mohanty, A. K., Kadner, R. J., & Wiener, M. C. (2003). Crystallization and initial X-ray diffraction of BtuB, the integral membrane cobalamin transporter of Escherichia coli. Acta crystallographica. Section D, Biological crystallography
- Chimento, D. P., Mohanty, A. K., Kadner, R. J., & Wiener, M. C. (2003). Substrate-induced transmembrane signaling in the cobalamin transporter BtuB. Nature structural biology