Goat TrueBlot®: Anti-Goat IgG HRP
Goat TrueBlot® IP / Western Blot
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Datasheet

Goat TrueBlot®: Anti-Goat IgG HRP

Mouse Monoclonal eB270

18-8814-31 18-8814-33
50 µL 200 µL
IP, WB
Goat
Mouse
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$119.00 /Per Item
$245.00 /Per Item
50 µL $119.00
200 µL $245.00
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Description

Background

Goat IgG TrueBlot® is a unique horseradish peroxidase conjugated Anti-Goat IgG monoclonal secondary antibody. Goat IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Goat IgG TrueBlot®, simply substitute the conventional HRP Anti-Goat IgG blotting reagent with Goat IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting. Goat IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of Goat IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Goat IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblot applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.

Application Note

Goat IgG HRP TrueBlot has been tested in western blot and immunoprecipitation and may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. Goat IgG TrueBlot® is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mLs). If used conservatively at 2.5mls/blot will yield enough reagent for 20 blots. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the Mouse, Goat and Sheep TrueBlot secondaries, but not with the Rabbit TrueBlot secondary. Use of protein A or G beads with the Rabbit TrueBlot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot.

Purity/Specificity

Goat TrueBlot® Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against Anti-Goat Serum. Reactivity is observed against native Goat IgG by both Western blot and ELISA.

Goat TrueBlot®: Anti-Goat IgG HRP - 18-8814-31
Anti-Goat IgG HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting
IP, WB
Goat
Mouse
Peroxidase (Horseradish) ULTRA
Monoclonal
eB270
IgG
Immunoprecipitation Kit
3.1 moles HRP ULTRA per mole of IgG
Liquid (sterile filtered)
1 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Proclin is added as an antimicrobial agent.
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Wet Ice
Store at -20 °C. This product is guaranteed for 1 year upon receipt, when handled and stored as instructed.
Expiration date is six (6) months from date of receipt.
Dominant-Negative Form of SIGIRR: SIGIRRΔE8 Promotes Tumor Growth Through Regulation of Metabolic Pathways. J Interferon Cytokine Res. ()
Applications
IP, Co-IP; WB, IB, PCA
Defective NET clearance contributes to sustained FXII activation in COVID-19-associated pulmonary thrombo-inflammation. eBioMedicine ()
Applications
WB, IB, PCA
Angiocrine IGFBP3 spatially coordinates IGF signaling during neonatal cardiac regeneration. bioRxiv Preprint ()
Applications
WB, IB, PCA
Protocol for in vitro BCR-mediated plasma cell differentiation and purification of chromatin-associated proteins. STAR Protoc. ()
Applications
WB, IB, PCA
Assembly of vascular smooth muscle cells in 3D aggregates provokes cellular quiescence. Exp Cell Res. ()
Applications
WB, IB, PCA
Broad-spectrum receptor tyrosine kinase inhibitors overcome de novo and acquired modes of resistance to EGFR-targeted therapies in colorectal cancer. Oncotarget ()
Applications
WB, IB, PCA
Adipose tissue-derived stromal cells are sources of cancer-associated fibroblasts and enhance tumor progression by dense collagen matrix. Int J Cancer. ()
Applications
WB, IB, PCA
Pink1 regulates FKBP5 interaction with AKT/PHLPP and protects neurons from neurotoxin stress induced by MPP. J Neurochem. ()
Applications
WB, IB, PCA
Environmental allergens induce allergic inflammation through proteolytic maturation of IL-33 Nat Immunol ()
Applications
WB, IB, PCA
Selected missense mutations impair frataxin processing in Friedreich ataxia. Annals of Clinical and Translational Neurology ()
Applications
IP, Co-IP; WB, IB, PCA
Improvement of Pharmacokinetic Profile of TRAIL via Trimer-Tag Enhances its Antitumor Activity in vivo. Scientific Reports ()
Applications
WB, IB, PCA
Borrelia burgdorferi BBK32 Inhibits the Classical Pathway by Blocking Activation of the C1 Complement Complex. PLOS Pathogens ()
Applications
IP, Co-IP
The nuclear form of glutathione peroxidase 4 colocalizes and directly interacts with protamines in the nuclear matrix during mouse sperm chromatin assembly. Spermatogenesis. ()
Applications
WB, IB, PCA
High-content analysis of proapoptotic EphA4 dependence receptor functions using small-molecule libraries. J Biolmol Screen ()
Applications
WB, IB, PCA
Host genetics and Chlamydia disease: prediction and validation of disease severity mechanisms. PLoS One. ()
Applications
WB, IB, PCA
Conversion of vascular endothelial cells into multipotent stem-like cells. Nat Med. ()
Applications
IP, Co-IP
PLTP regulates STAT3 and NFκB in differentiated THP1 cells and human monocyte-derived macrophages. Biochim Biophys Acta. ()
Applications
WB, IB, PCA
The p47 GTPases Iigp2 and Irgb10 regulate innate immunity and inflammation to murine Chlamydia psittaci infection. J Immunol. ()
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.