10X TTBS pH 7.5
10X TTBS
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Datasheet

10X TTBS pH 7.5

MB-013
1 L
WB
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$89.00 /Per Item
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Description

Background

Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Visit our newly expanded web site at www.rockland.com for methods using this and other buffers. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 1.0 M Tris HCl, 1.5 M Sodium Chloride and 0.1% (v/v) Tween-20 at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.

Application Note

TBST is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. May be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.

Purity/Specificity

10X TTBS solution was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Storage Condition

Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.

10X TTBS pH 7.5 (1.0 M Tris HCl 1.5 M Sodium Chloride 0.1% (v/v) Tween-20) - MB-013
TTBS, TBST, Tween Tris Buffered Saline
WB
Liquid (sterile filtered)
Ambient
Expiration date is six (6) months from date of opening.
10X
See application note.
None
None
Chatterjee A, Villarreal G Jr, Oh DJ, Kang MH, Rhee DJ. AMP-activated protein kinase regulates intraocular pressure, extracellular matrix, and cytoskeleton in trabecular meshwork. (2014)
Applications
WB, IB, PCA
Villarreal G Jr, Oh DJ, Kang MH, Rhee DJ. Coordinated regulation of extracellular matrix synthesis by the microRNA-29 family in the trabecular meshwork. (2011)
Applications
WB, IB, PCA

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If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.