Sheep IgG (H&L) Antibody
ELISA results of purified Rabbit Anti-Sheep IgG Antibody
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Datasheet

Sheep IgG (H&L) Antibody

Rabbit Polyclonal

613-4102
2 mg
ELISA, Other
Rabbit
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Description

Background

Anti-Sheep IgG (H&L) generated in rabbit detects sheep Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Note

Anti-Sheep IgG antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG and Sheep Serum.

Storage Condition

Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Anti-SHEEP IgG (H&L) (RABBIT) Antibody - 613-4102
Rabbit Anti-Sheep IgG Antibody, Rabbit Anti Sheep IgG Antibody
ELISA
Other
Sheep
Rabbit
Polyclonal
IgG
Liquid (sterile filtered)
Wet Ice
Expiration date is one (1) year from date of opening.
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Bian L et al. Rapid monitoring of vancomycin concentration in serum using europium (Ⅲ) chelate nanoparticle-based lateral flow immunoassay. (2021)
Applications
Lateral Flow Immunoassay (LFIA)
van der Staay Effects of chronic stress: a comparison between tethered and loose sows. (2010)
Applications
E, EIA
Erkens JH et al. A time-resolved fluoroimmunoassay for cortisol in unextracted bovine plasma or serum with optimized procedures to eliminate steroid binding protein interference and to minimize non-specific streptavidin-europium binding. (1998)
Applications
Other

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.