Rat IgG (H&L) Antibody DyLight™ 549 Conjugated
Dylight conjugated antibody - Western Blot
DyLight™ 549 Fluorescence Spectra.
Properties of DyLight™ Fluorescent Dyes.
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Datasheet

Rat IgG (H&L) Antibody DyLight™ 549 Conjugated

Goat Polyclonal

612-142-002 612-142-002-0.5
100 µg 5 x 100 µg
Dot Blot, IF
Rat
Goat
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$95.00 /Per Item
$162.00 /Per Item
100 µg $95.00
5 x 100 µg $162.00
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Application Note

Anti-Rat IgG DyLight™ 549 has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. This antibody will react with heavy chains of Rat IgG and with light chains of most Rat immunoglobulins.

Anti-RAT IgG (H&L) (GOAT) Antibody DyLight™ 549 Conjugated - 612-142-002
Goat Anti-Rat IgG DyLight 549™ Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 549™ Conjugation
Dot Blot
IF
Rat
Rat
Goat
DyLight™ 549
Polyclonal
IgG
Rat IgG whole molecule
4.8 moles DL549 per mole of IgG
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Igase M. et al. Establishment of rat anti-canine DEP domain containing 1B (DEPDC1B) monoclonal antibodies. J Vet Med Sci. (2020)
Applications
IF, Confocal Microscopy

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.