Rat IgG (H&L) Antibody DyLight™ 549 Conjugated

Dylight conjugated antibody - Western Blot

Properties of DyLight™ Fluorescent Dyes.

Datasheet

Rat IgG (H&L) Antibody DyLight™ 549 Conjugated

Name: Rat IgG (H&L) Antibody DyLight™ 549 Conjugated
Brand: Rockland Immunochemicals
Price: 395.0 USD
Availability: InStock

Goat Polyclonal

1 References

Item No. 612-142-002

Size: 100 µg

Applications: IF, Dot Blot

Reactivity: Rat

Host Species: Goat

$111.00 /Per Item

100 µg $111.00

5 x 100 µg $395.00

Add To Cart

Add to List

Bulk Orders

Availability: Ships next business day

Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Expand Background

Specifications for Rat IgG (H&L) Antibody DyLight™ 549 Conjugated

Product Details

Description: Goat Anti-Rat IgG (H&L) Antibody DyLight™ 549 Conjugated - 612-142-002

Synonyms: Goat Anti-Rat IgG DyLight 549™ Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 549™ Conjugation

Host Species: Goat

Specificity: IgG (H&L)

Conjugate: DyLight™ 549

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: Rat

Immunogen: Rat IgG whole molecule

Purity/Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. This antibody will react with heavy chains of Rat IgG and with light chains of most Rat immunoglobulins.

Application Details

Tested Applications: Dot Blot

Suggested Applications: IF - View References

Application Note: Anti-Rat IgG DyLight™ 549 has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Formulation

Physical State: Lyophilized

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 100 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (1)

Igase M. et al. (2020). Establishment of rat anti-canine DEP domain containing 1B (DEPDC1B) monoclonal antibodies. J Vet Med Sci.

Applications

IF, Confocal Microscopy

PubMed

Related Protocols

Adherent Cell Lysis Protocol

General Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Suspension Cultured Cell Lysis Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.