Datasheet is currently unavailable. Try again or CONTACT US
Mouse IgG1 Antibody Peroxidase Conjugated Pre-adsorbed
Goat Polyclonal
13 References
610-103-040
1 mg
Lyophilized
WB, ELISA, IHC
Mouse
Goat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Goat Anti-Mouse IgG1 (Gamma 1 chain) Antibody Peroxidase Conjugated (Min X Bv, Hu, and Rb Serum Proteins) - 610-103-040
Goat anti-mouse IgG1 antibody peroxidase conjugation, goat anti-mouse IgG1 (gamma 1) HRP conjugated antibody
Goat
IgG1
Peroxidase (HRP)
Polyclonal
IgG
Target Details
Mouse
Native Protein
Mouse IgG1 heavy chain
Anti-Mouse IgG1 HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Application Details
ELISA
IHC, WB
- View References
Anti-Mouse IgG1 Peroxidase Antibody has been tested by ELISA and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Mouse IgG1 Peroxidase Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG1 chain constitutes 66% of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Takada H et al. (2024). A role for the S4-domain containing protein YlmH in ribosome-associated quality control in Bacillus subtilis. Nucleic Acids Res.
Applications
WB, IB, PCA
Dimke H et al. (2023). Claudin-19 localizes to the thick ascending limb where its expression is required for junctional claudin-16 localization. Ann N Y Acad Sci.
Applications
IHC, ICC, Histology
Montero DA et al. (2023). A chimeric protein-based vaccine elicits a strong IgG antibody response and confers partial protection against Shiga toxin-producing Escherichia coli in mice. Front Immunol.
Applications
EIA, ELISA
Takada, H et al. (2022). Expression of Bacillus subtilis ABCF antibiotic resistance factor VmlR is regulated by RNA polymerase pausing, transcription attenuation, translation attenuation and (p)ppGpp. Nucleic Acids Research
Applications
WB, IB, PCA
Van Megen, WH et al. (2022). Differential parathyroid and kidney Ca2+-sensing receptor activation in autosomal dominant hypocalcemia 1. EbioMedicine
Applications
WB, IB, PCA
van Megen WH et al. (2022). Gentamicin Inhibits Ca2+ Channel TPRV5 and Induces Calciuresis Independent of the Calcium-Sensing Receptor-Claudin-14 Pathway. J Am Soc Nephrol.
Applications
WB, IB, PCA
Powers JM et al. (2021). Non-replicating adenovirus based Mayaro virus vaccine elicits protective immune responses and cross protects against other alphaviruses. PLoS Negl Trop Dis.
Applications
E, EIA
Frische S et al. (2021). Localization and regulation of claudin-14 in experimental models of hypercalcemia. Am J Physiol Renal Physiol.
Applications
WB, IB, PCA
Crowe-McAuliffe C et al. (2021). Structural basis for bacterial ribosome-associated quality control by RqcH and RqcP. Mol Cell.
Applications
WB, IB, PCA
Crowe-McAuliffe, C et al. (2021). Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens. Nature Communications
Applications
WB, IB, PCA
Kuata T et al. (2021). RelA-SpoT Homolog toxins pyrophosphorylate the CCA end of tRNA to inhibit protein synthesis. Mol Cell.
Applications
WB, IB, PCA
Prot-Bertoye C et al. (2021). Differential localization patterns of claudin 10, 16, and 19 in human, mouse, and rat renal tubular epithelia. Am J Physiol Renal Physiol.
Applications
IHC, ICC, Histology
Murina V et al. (2019). ABCF ATPases involved in protein synthesis, ribosome assembly and antibiotic resistance: structural and functional diversification across the tree of life. J Mol Biol.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
