Background

Anti-Mouse IgG1 Biotin Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG1 chain constitutes 66% of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Product Details

Goat Anti-Mouse IgG1 (Gamma 1 chain) Antibody Biotin Conjugated (Min X Bv, Hu, and Rb Serum Proteins) - 610-106-040
Goat anti-mouse IgG1 antibody biotin conjugation, goat anti-mouse IgG1 (gamma 1) biotin conjugated antibody
Goat
IgG1
Biotin
Polyclonal
IgG

Target Details

Mouse
Native Protein
Mouse IgG1 heavy chain
Anti-Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.

Application Details

ELISA
Mouse IgG1 Biotin Conjugated Antibody has been tested by ELISA and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.