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Mouse IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed
Donkey Polyclonal
7 References
610-741-124
100 µg
Lyophilized
WB, IHC, IF, Dot Blot, Multiplex
Mouse
Donkey
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Donkey Anti-Mouse IgG (H&L) Antibody DyLight™ 488 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) - 610-741-124
Donkey anti-Mouse IgG DyLight 488™ Conjugated Antibody, Donkey anti Mouse IgG Antibody DyLight 488™ Conjugation
Donkey
IgG (H&L)
DyLight™ 488
Polyclonal
IgG
Target Details
Mouse
Mouse IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of mouse IgG and with light chains of most mouse immunoglobulins.
Application Details
Dot Blot, WB
IF, IHC, Multiplex
- View References
Anti-Mouse IgG DyLight488 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Mouse IgG DyLight488 Antibody generated in donkey detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Rockhold JD et al. (2024). Everolimus alleviates CD4+ T cell inflammation by regulating autophagy and cellular redox homeostasis. Geroscience.
Applications
IF, Confocal Microscopy
Zukowski E et al. (2023). STAT3 modulates CD4+ T mitochondrial dynamics and function in aging. Aging Cell.
Applications
IF, Confocal Microscopy
Conway, R et al. (2022). Obesity and Fatty Acids Promote Mitochondrial Translocation of STAT3 Through ROS-Dependent Mechanisms. Frontiers in Aging
Applications
IF, Confocal Microscopy
Golenberg N, Squirrell JM, Bennin DA, et al. (2020). Citrullination regulates wound responses and tissue regeneration in zebrafish. J Cell Biol.
Applications
IF, Confocal Microscopy
Bharath LP, Agrawal M, McCambridge G, et al. (2020). Metformin Enhances Autophagy and Normalizes Mitochondrial Function to Alleviate Aging-Associated Inflammation. Cell Metab.
Applications
IF, Confocal Microscopy; IHC, ICC, Histology; Multiplex Assay
Watanabe T et al. (2018). Glucagon-like Peptide-1 Receptor Expression in the Pancreatic D Cells of Three Avian Species; White Leghorn Chickens, Northern Bobwhites, and Common Ostriches. J Poult Sci.
Applications
IHC, ICC, Histology
De Luigi A et al. (2015). Doxycycline hinders phenylalanine fibril assemblies revealing a potential novel therapeutic approach in phenylketonuria. Scientific Reports
Applications
IHC, ICC, Histology
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
