Mouse IgG F(c) Antibody DyLight™800 Conjugated

Datasheet

Mouse IgG F(c) Antibody DyLight™800 Conjugated

Name: Mouse IgG F(c) Antibody DyLight™800 Conjugated
Brand: Rockland Immunochemicals
Price: 111.0 USD
Availability: InStock

Rabbit Polyclonal

Item No. 610-445-003

Size: 100 µg

Applications: Dot Blot

Reactivity: Mouse

Host Species: Rabbit

$111.00 /Per Item

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Background

Anti-Mouse IgG F(c) DyLight antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

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Specifications for Mouse IgG F(c) Antibody DyLight™800 Conjugated

Product Details

Description: Rabbit Anti-Mouse IgG Fc Antibody DyLight™800 Conjugated - 610-445-003

Synonyms: Rabbit Anti-Mouse IgG F(c) Antibody DyLight™ 800 Conjugation, Rabbit Anti-Mouse IgG Fc Antibody DyLight™ 800 Conjugated, Rabbit Anti-Mouse IgG Fc Fragment DyLight™800 Conjugated Antibody

Host Species: Rabbit

Specificity: IgG Fc

Conjugate: DyLight™ 800

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: Mouse

Immunogen Type: Native Protein

Immunogen: Anti-Mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in rabbit.

Purity/Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).

Application Details

Tested Applications: Dot Blot

Application Note: Mouse IgG F(c) DyLight™800 Antibody has been tested by dot blot and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Formulation

Physical State: Lyophilized

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 100 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol

General Immunoblotting Protocol

Suspension Cultured Cell Lysis Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.