Human IgM (mu chain) Antibody Peroxidase Conjugated
ELISA Results of Goat Anti-Human IgM Peroxidase Conjugated Antibody
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Datasheet

Human IgM (mu chain) Antibody Peroxidase Conjugated

Goat Polyclonal

609-1307
1 mg
ELISA
Goat
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Description

Background

Anti-Human IgM (mu heavy chain) Peroxidase generated in goat detects specifically Human IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

Application Note

Anti-Human IgM (mu heavy chain) Peroxidase has been tested by ELISA and is suitable for western blot, immunohistochemistry, and immunoassays where specificity to the immunoglobulin mu chain region is desired. Optimal concentrations in immunoassays should be determined by the researcher.

Purity/Specificity

Human IgM (mu chain) Antibody, Peroxidase Conjugated, was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgM and Human Serum.  No reaction was observed against other human heavy or light chain proteins.

Anti-HUMAN IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated - 609-1307
Goat Anti-Human IgM (mu chain) Antibody Peroxidase Conjugation, Goat Anti-human IgM mu chain Antibody HRP Conjugated
ELISA
Human
Goat
Peroxidase (Horseradish)
Polyclonal
IgG
Human IgM whole molecule
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of opening.
Fox A. et al. Evidence of a significant secretory-IgA-dominant SARS-CoV-2 immune response in human milk following recovery from COVID-19. medRxiv (2020)
Applications
E, EIA
Jian L, Xiujian S, Yuangang Y, et al. Evaluation of antibody detection against the NDO-BSA, LID-1 and NDO-LID antigens as confirmatory tests to support the diagnosis of leprosy in Yunnan province, southwest China. Trans R Soc Trop Med Hyg. (2020)
Applications
E, EIA
Crawford et al. Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice. Scientific Reports (2017)
Applications
E, EIA
Serrano-Coll H et al. Anti-natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) antibodies as indicators of leprosy reactions and neuritis. Trans R Soc Trop Med Hyg. (2017)
Applications
E, EIA
McCallen J et al. Cross-reactivity of select PEG-binding antibodies to other polymers containing a CCO backbone. ACS Biomater Sci Eng. (2017)
Applications
E, EIA
Yang Q et al. Analysis of pre-existing IgG and IgM antibodies against polyethylene glycol (PEG) in the general population. Anal Chem. (2016)
Applications
E, EIA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.